Isolation and identification of bacteria from organic foods: Sensitivity to biocides and antibiotics

Food Control ◽  
2012 ◽  
Vol 26 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Miguel Angel Fernández-Fuentes ◽  
Elena Ortega Morente ◽  
Hikmate Abriouel ◽  
Rubén Pérez Pulido ◽  
Antonio Gálvez
Keyword(s):  
Isolation And Identification ◽  
Organic Foods ◽  
Identification Of Bacteria

scholarly journals ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH DECOMPOSING PIG (Sus scrofa) CARCASS

2021 ◽  
Vol 4 (2) ◽  
pp. e126
Author(s):  
Iyabo Victoria Olatubi ◽  
Olukemi Aromolaran ◽  
Samuel Tolani Joseph ◽  
Oluwafeyikemi Ajoke Adeleke
Keyword(s):  
Sus Scrofa ◽  
Skin Surface ◽  
Isolation And Identification ◽  
Forensic Biology ◽  
Gram Positive Bacteria ◽  
Average Temperature ◽  
Baseline Information ◽  
Identification Of Bacteria ◽  
Local Farm ◽  
Staphylococcus Spp

The emerging field of forensic biology has attempted to solve certain problems encountered when estimating post-mortem interval (PMI) by using predictable changes in the microbial and arthropod community structure. Pig (Sus scrofa) carcasses are widely used as animal models in clinical human studies. The objective of this study was to identify bacteria from the skin surface of pig carcass for possible use in forensic investigation. Three pigs (a suitable human substitute) were collected from a local farm and killed by suffocation and further place in a bush land for decomposition. 24hours later skin samples were collected and transported to the laboratory for the isolation of bacteria using standard pour plate techniques and identified using Bergey’s manual of systemic bacteriology. The experiment was conducted in February 2019 during the dry season of the year with an average temperature of 23.50c and relative humidity of 60.8% A total of fourteen (14) isolates were gotten from the pig carcass samples out of which four (4) were Gram-positive bacteria and the remaining ten (10) were Gram-negative. Staphylococcus spp. (28.6%) was the most abundant while Salmonella sp., Serratia sp., Klebsiella sp., Citrobacter sp. and Proteus sp. occurred at 14.3% each. This study focus on the type of bacteria communities during a decomposition process which will help provide baseline information in the application of forensic biology to determination of nature of death, abuse or neglect.

scholarly journals Isolation and identification of bacteria and parasites in glass eel (Anguilla spp.)

2021 ◽  
Vol 322 ◽  
pp. 02012
Author(s):  
Septyan Andriyanto ◽  
Hessy Novita ◽  
Tuti Sumiati ◽  
Taukhid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Prevalence Rate ◽  
Bacterial Species ◽  
Bacterial Identification ◽  
Biochemical Method ◽  
Isolation And Identification ◽  
Glass Eel ◽  
Pathogenic Agent ◽  
Identification Of Bacteria ◽  
Glass Eels

The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogyrus spp. (2,70%) and Gyrodactylus spp. (2,70%). Bacterial and parasites identified in glass eels need further verification on the epizootiology characteristic of each pathogenic agent.

Isolation and Identification of Bacteria from an Ice Wedge of the Mamontova Gora Glacial Complex (Central Yakutia)

2019 ◽  
Vol 46 (3) ◽  
pp. 234-241 ◽  
Author(s):  
S. N. Filippova ◽  
N. A. Surgucheva ◽  
T. V. Kolganova ◽  
M. Yu. Cherbunina ◽  
A. V. Brushkov ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Central Yakutia ◽  
Ice Wedge ◽  
Identification Of Bacteria ◽  
Glacial Complex

scholarly journals Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods

2017 ◽  
Vol 11 (1) ◽  
pp. 384-386 ◽  
Author(s):  
Mubarak Alfaresi ◽  
Bassam Mahboub
Keyword(s):  
Cystic Fibrosis ◽  
Bacterial Species ◽  
Transmembrane Conductance Regulator ◽  
Bacterial Identification ◽  
Molecular Method ◽  
Isolation And Identification ◽  
Pcr Product ◽  
Clinical Culture ◽  
Identification Of Bacteria ◽  
Cf Transmembrane Conductance Regulator

Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing. Results: All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning. Conclusion: This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.

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