Isolation and identification of bacteria and parasites in glass eel (Anguilla spp.)

The disease is the main agent that causes mortality of fish, especially during seed stages. The research aimed to find out bacteria and parasitic speciesin glass eel, Anguilla spp. Bacterial identification was carried out by a biochemical method. The prevalence of bacterial species was calculated using the El-Gohary et al. (2020) formula, while the results of bacterial identification from glass eel were Aeromonas spp., Vibrio spp., Enterococcus spp., Staphylococcus spp., Planococcus spp., Lactobacillus spp., Listeria spp., Citrbacterfreundii, Neisseria spp., Pseudomonas aeruginosa, Kurthia spp., Streptococcus spp., and Corynebacterium spp. It was found that the five highest prevalence rate was for Listeria spp. (39.64%), followed by Aeromonas spp. (26.13%), Staphylococcus spp. (16.22%), Corynebacterium spp. (5.41%), Lactobacillus spp. (2.70%), and the lowest prevalence rate was Streptococcus spp. (0.90%). The type of parasitic pathogen obtained was Trichodina spp. (2,70%), Dactylogyrus spp. (2,70%) and Gyrodactylus spp. (2,70%). Bacterial and parasites identified in glass eels need further verification on the epizootiology characteristic of each pathogenic agent.

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Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods

The Open Microbiology Journal ◽

10.2174/1874285801711010384 ◽

2017 ◽

Vol 11 (1) ◽

pp. 384-386 ◽

Cited By ~ 2

Author(s):

Mubarak Alfaresi ◽

Bassam Mahboub

Keyword(s):

Cystic Fibrosis ◽

Bacterial Species ◽

Transmembrane Conductance Regulator ◽

Bacterial Identification ◽

Molecular Method ◽

Isolation And Identification ◽

Pcr Product ◽

Clinical Culture ◽

Identification Of Bacteria ◽

Cf Transmembrane Conductance Regulator

Background: Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF. Objective: To compare phenotypic bacterial identification with a molecular method in a CF patient sputum. Methods: Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing. Results: All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning. Conclusion: This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.

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BACTERIA IN ANIMAL FEED AND THEIR SUSCEPTIBILITY TO ANTIMICROBIAL DRUGS

Archives of Veterinary Medicine ◽

10.46784/e-avm.v5i2.168 ◽

2012 ◽

Vol 5 (2) ◽

pp. 27-33

Author(s):

Igor M. Stojanov ◽

Milica M. Živkov Baloš ◽

Miloš C. Kapetanov ◽

Jasna Z. Prodanov Radulović ◽

Jelena M. Petrović

Keyword(s):

Animal Feed ◽

Bacterial Species ◽

Antibiotic Sensitivity ◽

Diffusion Method ◽

Isolation And Identification ◽

Antimicrobial Drugs ◽

Microbiological Safety ◽

Feed Control ◽

Identification Of Bacteria ◽

Feed Safety

Control of microbiological safety of animal feed is regulated by a law. The regulation is based on methodological standards for estimation of feed quality. Those methods are permanently amended and strive for continuous improvement of microbiological safety of feed. The question remains whether the presence or absence of some bacterial species is sufficient for proper assessment of feed safety. The answer to this question can be found not only in the examination of the number of bacteria in animal feed, but also in qualitative analysis of the isolated microorganisms. The sensitivity of bacteria from animal feed to antimicrobials is one of the qualitative characteristics of microorganisms that can affect food safety even if the feed meets the standards and is within the maximum permitted level. The subject of our work was to determine the presence of bacteria in animal feed with a purpose to examine if they possess lower sensibility to antibiotics. For testing antibiotic sensitivity the isolates from routine microbiological feed control were used. The animal feed was analysed by the standard methods for the isolation and identification of bacteria and determination of susceptibility was done using disc diffusion method according to CLSI. The following bacteria were detected: Escherichia coli, Enterobacter sp., Staphylococcus sp., Micrococcus sp., Bacillus cereus, Bacillus sp. The results showed that some isolates have reduced susceptibility to certain antimicrobial drugs (The resistance is different for different antibiotics and microorganism - from 8,33% to 100%). This finding gives a reason for further examination of certain characteristics of the bacteria found in animal feed and to detect their possible influence on ecology.

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Identification of bacteria by poly-aromatic hydrocarbons biosensors

10.1101/2021.11.27.470193 ◽

2021 ◽

Author(s):

Yaniv Shlosberg ◽

Yair Farber ◽

Salah Hasson ◽

Valery Bulatov ◽

Israel Schechter

Keyword(s):

Pathogenic Bacteria ◽

Polyaromatic Hydrocarbons ◽

Bacterial Species ◽

Environmental Water ◽

Intensity Increase ◽

Bacterial Identification ◽

Industrial Facilities ◽

Degrading Bacteria ◽

Identification Of Bacteria ◽

Poly Aromatic Hydrocarbons

Human health is consistently threatened by different species of pathogenic bacteria. To fight the spread of diseases, it is important to develop rapid methods for bacterial identification. Over the years, different kinds of biosensors were developed for this cause. Another environmental risk are polyaromatic hydrocarbons (PAHs) that may be emitted from industrial facilities and pollute environmental water and soil. One of the methods for their purification is conducted by the addition of bacteria that can degrade the PAHs, while the bacteria itself can be filtrated at the end of the process. Although many studies reported monitoring of the PAHs degradation by fluorescence, not much attention was dedicated to studying the influence of the PAHs on the intrinsic fluorescence of the degrading bacteria. In this work, we apply synchronous fluorescence (SF) measurements to study the ability of the 5 PAHs: 9Antracene carboxylic acid (9ACA), Pyrene, Perylene, Pentacene, and Chrysene to interact with bacteria and change its fluorescence spectra. We show that upon incubation of each PAH with the bacterium E.coli only the 2 PAHs 9ACA and Perylene cause an intensity decrease in the emission at λ = 300 – 375 nm, which derives from the emission of Tyrosine and Tryptophane (TT). Also, we show that upon incubation of 9ACA and Perylene with 5 different pathogenic bacteria, the intensity increase or decrease in the TT emission is unique to each bacterial species. Based on this observation, we suggest that the PAHs 9ACA and Perylene can be utilized as biosensors for bacterial identification.

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Isolation and identification of bacteria from four important poplar pests

Revista Colombiana de Entomología ◽

10.25100/socolen.v43i1.6644 ◽

2017 ◽

Vol 43 (1) ◽

pp. 34 ◽

Cited By ~ 2

Author(s):

Mustafa Yaman ◽

Ömer Ertürk ◽

Sabri Ünal ◽

Fazil Selek

Keyword(s):

Urban Areas ◽

Bacterial Flora ◽

Bacterial Identification ◽

Bacterial Isolates ◽

Isolation And Identification ◽

Disease Symptoms ◽

Ecological Control ◽

Identification Of Bacteria ◽

First Time ◽

Chemical Pesticides

In this study, the bacterial flora of important poplar pests was studied. This included Cryptorhynchus lapathi (Coleoptera: Curculionidae), Sciapteron tabaniformis (Lepidoptera: Sesiidae), Nycteola asiatica (Lepidoptera: Nolidae) and Gypsonoma dealbana (Lepidoptera: Tortricidae). The final goal was to propose alternative ecological control agents for poplar pests and decrease the undesirable effects caused by chemical pesticides in urban areas and urban forests. Forty-three bacteria were isolated from the larvae and adults exhibiting characteristic disease symptoms of these pests in five different localities for the first time. All bacterial isolates were cultured and identified using VITEK bacterial identification systems (VITEK® 2 GN ID card prod. no; 21341 and VITEK® 2 GP ID card prod. no; 21342, bioMerieux, Marcy l’Etoile). The members of the genera from Bacillaceae and Enterobacteriaceae families were most commonly isolated from both pest insects.

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Snow angels – the microbiology of freshly fallen snow: implications for immunocompromised patients

Journal of Water and Health ◽

10.2166/wh.2018.156 ◽

2018 ◽

Vol 16 (6) ◽

pp. 1029-1032 ◽

Cited By ~ 1

Author(s):

John E. Moore ◽

John McCaughan ◽

Jonathan Stirling ◽

Jane Bell ◽

B. Cherie Millar

Keyword(s):

Pseudomonas Aeruginosa ◽

Bacterial Species ◽

Public Spaces ◽

Viable Count ◽

Gram Positive ◽

Diverse Range ◽

Gram Negative ◽

Domestic Gardens ◽

Identification Of Bacteria ◽

Human Infections

Abstract The frequency of seasonal snowfall results in the transient covering of gardens/amenity sites/open public spaces, which encourages recreational interaction mainly with children. No data is available demonstrating the microbiological composition of such fallen snow and therefore a study was undertaken to examine the microbiology of snow from 37 sites, estimating (i) total viable count (TVC), (ii) identification of bacteria, and (iii) the presence of Pseudomonas aeruginosa. Mean TVC count of 8.3 colony-forming units (cfu)/ml snow melt water, 51.7 cfu/ml, 865 cfu/ml and 2,197 cfu/ml, was obtained for public amenity sites, domestic gardens, public open spaces and melting snow from public footpaths, respectively. No bacterial organisms (<10 cfu/ml) were detected in 5/14 (35.7%) open public spaces, 2/5 (40%) amenity sites and in 1/10 (10%) domestic gardens. Pseudomonas aeruginosa was not detected from any snow sample examined. Bacterial diversity consisted of 15 bacterial species (11 Gram-positive/four Gram-negative). The six Gram-positive genera identified from snow were Actinomyces, Bacillus, Brevibacillus, Micrococcus, Staphylococcus and Streptococcus. The four Gram-negative genera identified were Enterobacter, Pantoea, Pseudomonas and Xanthomonas. Bacillus licheniformis was the most commonly isolated organism from snow; it was isolated from every snow type. Snow may contain a diverse range of bacteria, many of which are capable of causing human infections.

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Bacteriological investigation of pyometra of Black Bengal goats obtained at slaughter

Bangladesh Veterinarian ◽

10.3329/bvet.v36i1-2.55744 ◽

2019 ◽

Vol 36 (1-2) ◽

pp. 1-7

Author(s):

ABMJ Uddin ◽

M Nooruzzaman ◽

T Akter ◽

MAHNA Khan ◽

MM Hossain

Keyword(s):

Bacterial Species ◽

Capra Hircus ◽

Mixed Infections ◽

Bacillus Sp ◽

Chain Reaction ◽

Isolation And Identification ◽

Bacteriological Investigation ◽

Identification Of Bacteria ◽

Polymerase Chain ◽

Phosphate Buffered Saline

Nine uteri of Black Bengal goats (Capra hircus) affected with pyometra were collected from three slaughterhouses at Kishoreganj district of Bangladesh. Both horns of each uterus were washed with phosphate buffered saline for isolation and identification of bacteria and its load. The bacterial loads in the uterus were high, ranging between 1 × 107 and 2.8 × 107. Six different bacterial species were identified and confirmed by Polymerase Chain Reaction (PCR). There were five Escherichia coli, six Streptococcus sp., five Staphylococcus sp, one Salmonella sp., one Pasteurella sp. and one Bacillus sp. All had mixed infections containing two or three types of bacterial pathogens. Further studies are needed for the virulence determination and antibiogram profiles. The Bangladesh Veterinarian (2019) 36(1 - 2): 1-7

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Detection of Pseudomonas aeruginosa in Hospital Contamination

Baghdad Science Journal ◽

10.21123/bsj.5.3.353-356 ◽

2008 ◽

Vol 5 (3) ◽

pp. 353-356

Author(s):

Baghdad Science Journal

Keyword(s):

Pseudomonas Aeruginosa ◽

Isolation And Identification ◽

Identification Of Bacteria ◽

Hospital Wards

The Present investigation includes the isolation and identification of Pseudomonas aeruginosa for different cases of hospital contamination from 1/ 6/2003 to 30/9/2004, the identification of bacteria depended on morphological , cultural and biochemical characters, 37 of isolates were diagnosed from 70 smears from wounds and burns beside 25 isolates were identified from 200 smears taken from operation theater and hospital wards including the floors , walls , sources of light and operation equipment the sensitivity of all isolates to antibiotic were done , which exhibited complete sensitivity to Ciprofloxacin , Ceftraixon, Tobromycin and Gentamysin ,while they were complete resist to Amoxcillin , Tetracyclin , Nitrofurantion , Clindamycin Cefotoxam and Methoprim.

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Characterization of pathogenic bacteria in eel Anguilla bicolor bicolor

Jurnal Akuakultur Indonesia ◽

10.19027/jai.17.1.94-103 ◽

2018 ◽

Vol 17 (1) ◽

pp. 94

Author(s):

Dinamella Wahjuningrum ◽

Acep Muhamad Hidayat ◽

Tatag Budiardi

Keyword(s):

Streptococcus Agalactiae ◽

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Biochemical Characterization ◽

Average Length ◽

Average Weight ◽

Isolation And Identification ◽

Glass Eel ◽

Identification Of Bacteria

ABSTRACTThis research aimed to characterize bacteria caused disease in eel Anguilla bicolor bicolor. The research was conducted in two steps. The first step included the isolation and identification of bacteria from the disease infected glass eel (average body length: 5.0±0.5 cm, average weight: 0.5±0.1 g). The observation were colony and cell morphology, physiology, and biochemical characterization of bacteria, hemolysis test, and bacteria identification performed by KIT API 20 E, KIT API 20 Strep, and KIT API 20 Listeria. The second step was Koch’s postulate, tested on healthy elver with an average length of 15.00±0.65 cm and weight of 3.00±0.75 g. The results showed three dominant species of bacteria suspected as a causative agent in eel, namely: Aeromonas hydrophila, Streptococcus agalactiae, and Listeria grayi. Koch’s postulates test proved that the Aeromonas hydrophila and Streptococcus agalactiae were virulent to Anguilla bicolor bicolor. Thus, A.hydrophila and S. agalactiae were disease-causing agent bacteria in eel. Keywords: Anguilla bicolor bicolor, bacteria, A. hydrophila, S. agalactiae ABSTRAKPenelitian ini bertujuan untuk mengkarakterisasi bakteri penyebab penyakit pada ikan sidat Anguilla bicolor bicolor. Penelitian ini dilakukan dalam dua tahap. Tahap pertama meliputi isolasi dan identifikasi bakteri dari ikan sidat kondisi sakit pada stadia glass eel. Ukuran panjang ikan sidat rata-rata 5±0,5 cm dan bobot rata-rata 0,5±0,08 g, pengamatan bentuk morfologi koloni dan morfologi sel, karakterisasi fisiologi, dan biokimia bakteri, serta uji hemolisis, dan identifikasi jenis bakteri dengan KIT API 20 E, KIT API 20 Strep, dan KIT API 20 Listeria. Tahap kedua yaitu uji postulat Koch pada ikan sidat kondisi sehat stadia elver yang berukuran panjang rata-rata 15±0,65 cm dan bobot rata-rata 3±0,75 g. Hasil penelitian diperoleh tiga jenis bakteri dominan yaitu Aeromonas hydrophila, Streptococcus agalactiae, dan Listeria grayi. Uji postulat Koch membuktikan bahwa bakteri A. hydrophila dan S. agalactiae bersifat virulen pada ikan sidat Anguilla bicolor bicolor. Dengan demikian maka bakteri A. hydrophila dan S. agalactiae sebagai bakteri penyebab penyakit pada ikan sidat. Kata kunci: Anguilla bicolor bicolor, bakteri, A. hydrophila, S. agalactiae

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