scholarly journals ISOLATION AND IDENTIFICATION OF BACTERIA ASSOCIATED WITH DECOMPOSING PIG (Sus scrofa) CARCASS

2021 ◽  
Vol 4 (2) ◽  
pp. e126
Author(s):  
Iyabo Victoria Olatubi ◽  
Olukemi Aromolaran ◽  
Samuel Tolani Joseph ◽  
Oluwafeyikemi Ajoke Adeleke
Keyword(s):  
Sus Scrofa ◽  
Skin Surface ◽  
Isolation And Identification ◽  
Forensic Biology ◽  
Gram Positive Bacteria ◽  
Average Temperature ◽  
Baseline Information ◽  
Identification Of Bacteria ◽  
Local Farm ◽  
Staphylococcus Spp

The emerging field of forensic biology has attempted to solve certain problems encountered when estimating post-mortem interval (PMI) by using predictable changes in the microbial and arthropod community structure. Pig (Sus scrofa) carcasses are widely used as animal models in clinical human studies. The objective of this study was to identify bacteria from the skin surface of pig carcass for possible use in forensic investigation. Three pigs (a suitable human substitute) were collected from a local farm and killed by suffocation and further place in a bush land for decomposition. 24hours later skin samples were collected and transported to the laboratory for the isolation of bacteria using standard pour plate techniques and identified using Bergey’s manual of systemic bacteriology. The experiment was conducted in February 2019 during the dry season of the year with an average temperature of 23.50c and relative humidity of 60.8% A total of fourteen (14) isolates were gotten from the pig carcass samples out of which four (4) were Gram-positive bacteria and the remaining ten (10) were Gram-negative. Staphylococcus spp. (28.6%) was the most abundant while Salmonella sp., Serratia sp., Klebsiella sp., Citrobacter sp. and Proteus sp. occurred at 14.3% each. This study focus on the type of bacteria communities during a decomposition process which will help provide baseline information in the application of forensic biology to determination of nature of death, abuse or neglect.

Isolation and Identification of Bacteria from Marine Biofilms

2005 ◽  
Vol 277-279 ◽  
pp. 612-617 ◽  
Author(s):  
Yoo Kyung Lee ◽  
Kae Kyoung Kwon ◽  
Kyeung Hee Cho ◽  
Jae Hyun Park ◽  
Hong Kum Lee
Keyword(s):  
Culturable Bacteria ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Rdna Sequences ◽  
Marine Biofilms ◽  
Antifouling Agent ◽  
Identification Of Bacteria ◽  
Invertebrate Larvae

In the marine environment, biofilms cover most of the subtidal and intertidal solid surfaces. Culturable bacteria forming marine biofilms were isolated on artificial substrate called acrylic coupons. The bacterial isolates were identified through a comparison of 16S rDNA sequences. A total of 115 strains were cultured and identified, 45 of which showed the same sequences with other strains. Therefore, 70 strains were finally identified. The bacterial isolates belonged to a–Proteobacteria (32 isolates), g–Proteobacteria (12 isolates), CFB group bacteria (4 isolates), high GC Gram-positive bacteria (9 isolates), and low GC Gram-positive bacteria (13 isolates). The bacterial isolates may be used as standard bacteria to test new antifouling agent. They may also be utilized as useful bacteria to enhance the settlement of commercial algae and invertebrate larvae for aquaculture.

scholarly journals Isolation and Identification of Bacteria in the Mouth Before and After Ablution

2021 ◽  
Vol 3 (3) ◽  
pp. 132-138
Author(s):  
Fitriah Fitriah ◽  
Mochammad Erwin Rachman ◽  
Sri Wahyuni Gayatri ◽  
Fendy Dwimartyono ◽  
Hasta Handayani Idrus
Keyword(s):  
Oral Health ◽  
The Body ◽  
Personal Hygiene ◽  
Pseudomonas Sp ◽  
Normal Flora ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Before And After ◽  
Identification Of Bacteria ◽  
Number Of Bacteria

Background: The oral is the gateway for the entry of various kinds of microorganisms into the body, with the prevalence of people having dental and oral problems in Indonesia increasing every year. The normal flora of the oral acts as a body defense, but it can cause disease due to predisposing factors, namely oral hygiene. Therefore, it is necessary to find an alternative in maintaining oral health. Islam is a religion that emphasizes personal hygiene, such as performing ablution. Content: The types of bacteria found in the oral before ablution was 33.33% Pseudomonas sp., 6.67% Lactobacillus sp., 3.33% Streptococcus sp. and 0.14% Staphylococcus sp. while the types of bacteria found in the oral after ablution were 26,8% Pseudomonas sp., 20% Lactobacillus sp., 5% Streptococcus sp. and 2% Staphylococcus sp. Conclusion: There was a change in the number of bacteria, namely an increase in gram-positive bacteria in the oral after ablution.

scholarly journals Isolation and identification of bacteria from tracheas and lungs of buffaloes in Dinajpur

2013 ◽  
Vol 2 (1) ◽  
pp. 31-33 ◽  
Author(s):  
Md Zulfekar Ali ◽  
Shirin Sultana
Keyword(s):  
Lung Diseases ◽  
Bacterial Isolate ◽  
Pasteurella Haemolytica ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Different Types ◽  
Identification Of Bacteria ◽  
Ferment Lactose ◽  
Staphylococcus Spp ◽  
And Control

The aim of the present study was the isolation and identification of bacteria from trachea and lung of slaughtered buffaloes. A total of 160 swab samples were collected from tracheas (80) and lungs (80) freshly slaughtered buffeloes from the slaughter houses of Dinajpur Sadar, Dinajpur, Bangladesh, during the period from June 2011 to February 2012. Among them, 9 tracheas (out of 80 samples) and 30 lungs (out of 80 samples) showed visible lesions. Bacterial isolates were identified based on their growth, morphological and biochemical characteristics. The most frequent (5%) bacterial isolate in tracheas was Staphylococcus spp. Pasteurella haemolytica and Staphylococcus spp. were isolated from different types of lung lesions in a frequency of 5% and 16.25%, respectively. P. haemolytica showed ?- haemolysis on blood agar and did not ferment lactose and dulcitol whereas dextrose, manitol and maltose were fermented with the production of acid. This investigation would be helpful for veterinarian for the detection, treatment and control of tracheal and lung diseases of buffaloes. DOI: http://dx.doi.org/10.3329/sjm.v2i1.15211 Stamford Journal of Microbiology, Vol.2(1) 2012: 31-33

scholarly journals Isolation and Identification of Pathogenic Gram-Positive Bacteria from Egg Shell of Hen and to See Their Antimicrobial Susceptibility Pattern

2016 ◽  
Vol 6 (1) ◽  
pp. 15-18 ◽  
Author(s):  
Jannatul Fardows ◽  
Abu Bakar Siddique ◽  
Adneen Moureen ◽  
Tasmin Afroz Binte Islam ◽  
Nasrin Farhana ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Major Public Health Problem ◽  
Isolation And Identification ◽  
Gram Positive ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Gram Positive Bacteria ◽  
Source Of Infection ◽  
Egg Shell ◽  
Staphylococcus Spp

Background: Food-borne disease is a major public health problem affecting developed as well as developing countries. Inaccurately treated eggs can be one of its causes. So we designed this study to observe the possibility of transmission of pathogenic Gram-positive bacteria from market eggs to the community.Objectives: To identify different Gram-positive bacteria in eggs and to observe their antimicrobial susceptibility.Materials and Methods: This observational study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka. Shells of 150 eggs collected from different markets of Dhaka city were tested. Bacteria were isolated and identified by culture and relevant biochemical tests.Results: Out of 150 egg shells, 120 (80%) yielded growth of different bacteria. Of them, Staphylococcus spp. were 80 (66.67%), Streptococcus spp. 8 (6.67%), Bacillus subtilis 20 (16.67%) and Bacillus cereus 12 (10%). Out of 80 Staphylococcus spp., 30 (25%) were Staphylococcus aureus and 50 (41.67%) were Staphylococcus saprophyticus. Most of the Gram-positive bacteria were sensitive to ciprofloxacin, ceftriaxone and imipenem. No MRSA and VRSA were found.Conclusion: It can be concluded from this study that Gram-positive bacteria from market eggs may be an important source of infection to the community.J Enam Med Col 2016; 6(1): 15-18

scholarly journals Isolation and identification of some genera and species of bacteria and fungi from conjunctiva in cattle in Al-Diwaniya city

2013 ◽  
Vol 12 (2) ◽  
pp. 55
Author(s):  
H. H. H. Handool
Keyword(s):  
Bacterial Species ◽  
Gram Negative Bacteria ◽  
Isolation And Identification ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Moraxella Bovis ◽  
Aspergillus Fumigates ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Penicillium Spp

This study was designed to identify some genera and species of bacteria and fungi that infect conjunctiva in cattle, as the studies on this subject are few somewhat, especially those that include isolating fungi that infect the conjunctiva as rare cases and that may be caused by low body immunity after topical corticosteroid and antibacterial therapy to an inflamed eye, This study included examination of (100) eye swabs, from cows from different ages and regions in diwaniya city. This study extended from 1/5/2012 – 27/11/ 2012. The samples were collected from clinically healthy and infected eyes of animals. (7) bacterial species were isolated they included Staphylococcus spp. 26.4% , E. coli 24.8%, Staphylococcus aureus 19% , Moraxella bovis 9.9% , Streptococcus spp. 8.3%, , , Pseudomonas spp. 8.3%, Proteus spp. 3.3%. The isolated bacteria were 121, Gram-positive bacteria were 63 (52.1%) while Gram-negative bacteria 58 (47.9%). Many types of bacteria isolated from healthy swabs 75 (62%) and 46 (38.1%) from infected swabs. The study also included isolation and identification of some species of fungi associated with the mycotic conjunctivitis in cattle, the observed study Aspergillus niger was the most frequent species isolated and was accounted to 37.5% of all isolates, while other fungi included Aspergillus fumigates 25% , Alternaria spp. 25% and Penicillium spp. 12.5%.

Influence of Cell Wall Composition on the Fossilisation of Bacteria and the Implications for the Search for Early Life Forms

1997 ◽  
Vol 161 ◽  
pp. 491-504 ◽  
Author(s):  
Frances Westall
Keyword(s):  
Cell Wall ◽  
Life Forms ◽  
Cation Binding ◽  
Positive Bacterium ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Transmission Electron ◽  
Hydrothermal Sediments ◽  
Identification Of Bacteria ◽  
The Difference

AbstractThe oldest cell-like structures on Earth are preserved in silicified lagoonal, shallow sea or hydrothermal sediments, such as some Archean formations in Western Australia and South Africa. Previous studies concentrated on the search for organic fossils in Archean rocks. Observations of silicified bacteria (as silica minerals) are scarce for both the Precambrian and the Phanerozoic, but reports of mineral bacteria finds, in general, are increasing. The problems associated with the identification of authentic fossil bacteria and, if possible, closer identification of bacteria type can, in part, be overcome by experimental fossilisation studies. These have shown that not all bacteria fossilise in the same way and, indeed, some seem to be very resistent to fossilisation. This paper deals with a transmission electron microscope investigation of the silicification of four species of bacteria commonly found in the environment. The Gram positiveBacillus laterosporusand its spore produced a robust, durable crust upon silicification, whereas the Gram negativePseudomonas fluorescens, Ps. vesicularis, andPs. acidovoranspresented delicately preserved walls. The greater amount of peptidoglycan, containing abundant metal cation binding sites, in the cell wall of the Gram positive bacterium, probably accounts for the difference in the mode of fossilisation. The Gram positive bacteria are, therefore, probably most likely to be preserved in the terrestrial and extraterrestrial rock record.

Bio-P and non-bio-P bacteria identification by a novel microbial approach

1999 ◽  
Vol 39 (6) ◽  
pp. 13-20 ◽  
Author(s):  
Philip L. Bond ◽  
Jürg Keller ◽  
Linda L. Blackall
Keyword(s):  
In Situ Hybridisation ◽  
Microbial Populations ◽  
Biological Phosphorus Removal ◽  
Gram Positive Bacteria ◽  
P Content ◽  
Identification Of Bacteria ◽  
Widespread Belief ◽  
Biological Phosphorus ◽  
P Removal

Culturing bacteria from activated sludge with enhanced biological phosphorus removal (EBPR) has strongly implicated Acinetobacter with the process. However, using fluorescent in-situ hybridisation (FISH) probing to analyse microbial populations, we have shown evidence opposing this widespread belief. We describe the phosphorus (P) removing performance and microbial population analyses of sludges obtained in a laboratory scale EBPR reactor. Two sludges with extremely high P removing capabilities were examined, the P content of these sludges was 8.6% (P sludge) and 12.3% (S sludge) of the MLSS. Identification of bacteria using FISH probing indicated both sludges were dominated by microbes from the beta proteobacteria and high mol% G+C Gram positive bacteria. Acinetobacter could make up only a small proportion of the cells in these sludges. Sludge with extremely poor P removal (P content of 1.5%, referred to as T sludge) was then generated by reducing the P in the influent. Bacteria resembling the G-bacteria became abundant in this sludge and these were identified using FISH probing. The anaerobic transformations of the T and P sludges correlated well with that of the non-EBPR and EBPR biological models respectively, indicating that bacteria in the T sludge have the potential to inhibit P removal in EBPR systems.

scholarly journals Diversity and Antimicrobial Activity of Culturable Fungal Endophytes in Solanum mauritianum

2020 ◽  
Vol 17 (2) ◽  
pp. 439 ◽  
Author(s):  
Sharon Pelo ◽  
Vuyo Mavumengwana ◽  
Ezekiel Green
Keyword(s):  
Deoxyribonucleic Acid ◽  
Fungal Endophytes ◽  
Antimycobacterial Activity ◽  
Great Activity ◽  
Isolation And Identification ◽  
Gram Positive Bacteria ◽  
Crude Extracts ◽  
Solanum Mauritianum ◽  
Fusarium Sp ◽  
Respective Host

Plant endophytes are microbial sources of bioactive secondary metabolites, which mimic the natural compounds chemistry of their respective host plants in a similar manner. This study explored the isolation and identification of fungal endophytes, and investigated the antibacterial and antimycobacterial activity of their crude extracts. Fungal endophytes were isolated from Solanum mauritianum, identified using morphological traits and internal transcribed spacer ribosomal-deoxyribonucleic acid (ITS-rDNA) sequence analysis. Eight fungal endophytes were identified as Aureobasidium pullulans, Paracamarosporium leucadendri, Cladosporium sp., Collectotrichum boninense, Fusarium sp., Hyalodendriella sp., and Talaromyces sp., while Penicillium chrysogenum was isolated from the leaves and unripe fruits. Good activity was observed for the crude extracts of Paracamarosporium leucadendri inhibiting Mycobacterium bovis, Klebsiella pneumoniae, and Pseudomonas aeruginosa at 6 µg/mL. Crude extracts of Fusarium sp., showed activity at 9 μg/mL against M. bovis, M. smegmatis and K. pneumonia. In general, the crude extracts showed great activity against Gram-negative and Gram-positive bacteria and novel results for two mycobacteria species M. bovis and M. smegmatis. The results provide evidence of diverse fungal endophytes isolated from Solanum mauritianum, and evidence that fungal endophytes are a good source of bioactive compounds with pharmaceutical potential, particularly against Mycobacterium tuberculosis.

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