Development of a direct loop-mediated isothermal amplification (LAMP) assay for rapid and simple on-site detection of chicken in processed meat products

Food Control ◽  
2019 ◽  
Vol 98 ◽  
pp. 194-199 ◽  
Author(s):  
SuYeon Sul ◽  
Mi-Ju Kim ◽  
Hae-Yeong Kim
Keyword(s):  
Meat Products ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Processed Meat ◽  
Loop Mediated Isothermal Amplification

Loop-Mediated Isothermal Amplification (LAMP) for the Detection of Horse Meat in Meat and Processed Meat Products

2014 ◽  
Vol 8 (6) ◽  
pp. 1576-1581 ◽  
Author(s):  
Celine Zahradnik ◽  
Roland Martzy ◽  
Robert L. Mach ◽  
Rudolf Krska ◽  
Andreas H. Farnleitner ◽  
...  
Keyword(s):  
Meat Products ◽  
Isothermal Amplification ◽  
Processed Meat ◽  
Horse Meat ◽  
Loop Mediated Isothermal Amplification

scholarly journals Combination of loop-mediated isothermal amplification and AuNP-oligoprobe colourimetric assay for pork authentication in processed-meat products

2020 ◽  
Author(s):  
Pattanapong Thangsunan ◽  
Sasithon Temisak ◽  
Phattaraporn Morris ◽  
Leonardo Rios-Solis ◽  
Nuttee Suree
Keyword(s):  
Limit Of Detection ◽  
Meat Products ◽  
High Sensitivity ◽  
Cross Reactivity ◽  
Isothermal Amplification ◽  
Processed Meat ◽  
High Concentration ◽  
Food Authentication ◽  
Loop Mediated Isothermal Amplification ◽  
Halal Food

AbstractPork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well as those who are allergic to pork meat and its derivatives. Accurate pork authentication is of great importance to assist this demographic group of people in making decision on their product purchase. The aim of this study was to develop a new analytical method for pork authentication in processed-meat products based on a combination of loop-mediated isothermal amplification (LAMP) and AuNP-nanoprobe colourimetric assay. The LAMP conditions were first optimised to obtain the highest yield of amplified DNA products within the shortest time. Oligoprobe-functionalised AuNPs were then hybridised with LAMP-DNA amplicons, and subsequently challenged with MgSO4 at a high concentration to induce AuNP aggregation. In the presence of pork DNA, the colloidal AuNPs-probe remained unchanged in its red colour, which indicates the dispersion of AuNPs. In contrast, in the absence of pork DNA, the colour was changed to colourless as a result from the aggregation of AuNPs. The LAMP-AuNP-nanoprobe assay offers a high sensitivity with a limit of detection as low as 100 pg of pork DNA. The assay is highly specific to pork content without cross-reactivity with the other meat species tested. The assay developed herein can become a simple, inexpensive, precise, and rapid analytical tool for small laboratories or the general public interested in halal food authentication.

scholarly journals Combination of Loop-Mediated Isothermal Amplification and AuNP-Oligoprobe Colourimetric Assay for Pork Authentication in Processed Meat Products

2020 ◽  
Author(s):  
Pattanapong Thangsunan ◽  
Sasithon Temisak ◽  
Phattaraporn Morris ◽  
Leonardo Rios-Solis ◽  
Nuttee Suree
Keyword(s):  
Limit Of Detection ◽  
Meat Products ◽  
High Sensitivity ◽  
Cross Reactivity ◽  
Isothermal Amplification ◽  
Processed Meat ◽  
High Concentration ◽  
Food Authentication ◽  
Loop Mediated Isothermal Amplification ◽  
Halal Food

Abstract Pork adulteration is a major concern for Muslims and Jews whose diets are restricted by religious beliefs, as well as those who are allergic to pork meat and its derivatives. Accurate pork authentication is of great importance to assist this demographic group of people in making decision on their product purchase. The aim of this study was to develop a new analytical method for pork authentication in processed meat products based on a combination of loop-mediated isothermal amplification (LAMP) and AuNP-nanoprobe colourimetric assay. The LAMP conditions were first optimised to obtain the highest yield of amplified DNA products within the shortest time. Oligoprobe-functionalised AuNPs were then hybridised with LAMP-DNA amplicons and subsequently challenged with MgSO4 at a high concentration to induce AuNP aggregation. In the presence of pork DNA, the colloidal AuNP-probe remained unchanged in its red colour, which indicates the dispersion of AuNPs. In contrast, in the absence of pork DNA, the colour was changed to colourless as a result from the aggregation of AuNPs. The LAMP-AuNP-nanoprobe assay offers a high sensitivity with a limit of detection as low as 100 pg of pork DNA. The assay is highly specific to pork content without cross-reactivity with the other meat species tested. The assay developed herein can become a simple, inexpensive, precise, and rapid analytical tool for small laboratories or the general public interested in halal food authentication.

scholarly journals Loop-mediated isothermal amplification (LAMP) for rapid detection of L. monocytogenes in meat

10.5219/1165 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 800-805
Author(s):  
Yuliya Yushina ◽  
Anzhelika Makhova ◽  
Elena Zayko ◽  
Dagmara Bataeva
Keyword(s):  
Foodborne Pathogens ◽  
Meat Products ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Meat Industry ◽  
Safety Testing ◽  
Loop Mediated Isothermal Amplification ◽  
Specificity And Sensitivity ◽  
Detection Assay ◽  
Highly Sensitive

There is a continued need to develop improved rapid methods for detection of foodborne pathogens. Rapid and sensitive methods for enumeration of Listeria monocytogenes are important for microbiological food safety testing purpose. The aim of this project was to evaluate a commercial loop-mediated isothermal amplification (LAMP) based system with bioluminescence, named as 3M™ Molecular Detection Assay (MDA), was validated for the detection of L. monocytogenes in food products with a standard GOST 32031-2012 method as reference. The results of this study revealed that a commercial LAMP-based method performed equally effective compared with method, showing from 94% to 100% specificity and sensitivity, respectively. The LAMP-based method was shown to be rapid and reliable detection technique for L. monocytogenes present at low numbers (10 CFU.g-1) on raw meat and meat products and can be applicable in meat industry. Thus, compared with the microbiological method based GOST 32031-2012, the LAMP assay is a relatively rapid and highly sensitive method for detecting L. monocytogenes and will facilitate the surveillance for contamination of L. monocytogenes in food. The 3M MDS result and culture-based detection (GOST 32031-2012) did not differ significantly (p >0.05) regarding the number of positive samples.

scholarly journals Development of a loop-mediated isothermal amplification assay for the detection of Tilletia controversa based on genome comparison

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Somayyeh Sedaghatjoo ◽  
Monika K. Forster ◽  
Ludwig Niessen ◽  
Petr Karlovsky ◽  
Berta Killermann ◽  
...  
Keyword(s):  
Test Performance ◽  
Genomic Dna ◽  
Lamp Assay ◽  
Fungal Species ◽  
Isothermal Amplification ◽  
Genome Comparison ◽  
Performance Study ◽  
Loop Mediated Isothermal Amplification ◽  
Tilletia Controversa ◽  
Genomic Regions

AbstractTilletia controversa causing dwarf bunt of wheat is a quarantine pathogen in several countries. Therefore, its specific detection is of great phytosanitary importance. Genomic regions routinely used for phylogenetic inferences lack suitable polymorphisms for the development of species-specific markers. We therefore compared 21 genomes of six Tilletia species to identify DNA regions that were unique and conserved in all T. controversa isolates and had no or limited homology to other Tilletia species. A loop-mediated isothermal amplification (LAMP) assay for T. controversa was developed based on one of these DNA regions. The specificity of the assay was verified using 223 fungal samples comprising 43 fungal species including 11 Tilletia species, in particular 39 specimens of T. controversa, 92 of T. caries and 40 of T. laevis, respectively. The assay specifically amplified genomic DNA of T. controversa from pure cultures and teliospores. Only Tilletia trabutii generated false positive signals. The detection limit of the LAMP assay was 5 pg of genomic DNA per reaction. A test performance study that included five laboratories in Germany resulted in 100% sensitivity and 97.7% specificity of the assay. Genomic regions, specific to common bunt (Tilletia caries and Tilletia laevis together) are also provided.

Sign in / Sign up

Export Citation Format

Share Document