Verbascum sinaiticum: A Rich Source of Antioxidant Phenylethanoid Glycosides

2020 ◽  
Vol 10 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Humaira Yasmeen Gondal ◽  
Roshan Zamir ◽  
Muhammad Nisar ◽  
Muhammad Iqbal Choudhary
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Water Soluble ◽  
Antioxidant Potential ◽  
Active Fraction ◽  
Phenylethanoid Glycosides ◽  
Active Constituents ◽  
Soluble Part

Background: The genus Verbascum is well documented for its antioxidant potential but Verbascum sinaiticum is comparatively less studied plant. The current study was carried out to search for antioxidant nutraceuticals from this species. Objective: To explore the antioxidant potential of Verbascum sinaiticum and to identify its active constituents. Methods: The methanolic extract of air-dried aerial part of the Verbascum sinaiticum was partitioned with hexane, chloroform and ethyl acetate. The water-soluble part of ethyl acetate afforded six phenylethanoid glycosides by repeated chromatography over Sephadex LH-20, silica gel and ODS columns. Antioxidant activity of solvent extracts and isolated constituents were evaluated by DPPH, ABTS and FRAP assays. Results: Six phenylethanoid glycosides was isolated and characterized as Verbascoside, Eukovoside, Martynoside, Jionoside D, Campneoside I and Campneoside II, from the most active fraction. Conclusion: Verbascum sinaiticum demonstrated prospective antioxidant activity. The watersoluble part of EtOAc (WSEAE) was found the most active extract whereas Verbascoside was identified as the most potent constituent. All isolated compounds exhibited significant antioxidant activity whereas their synergistic effect was found prominent in the parent fraction.

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF HABENARIA PECTINATA

2020 ◽  
pp. 68-71
Author(s):  
CHARANJIT KAUR ◽  
RAJESH KUMAR ◽  
GURVINDER SINGH ◽  
CHANDER MOHAN
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Flavonoid Content ◽  
Anti Oxidant

Objective: This study was designed to evaluate the antioxidative potential of tubers of Habenaria pectinata. Methods: The tubers of H. pectinata were extracted using hexane, ethyl acetate, methanol, and water as solvents. The anti-oxidant potential of extracts was evaluated using free radical scavenging and reducing power assays. The most active methanolic extract was then fractionated into four fractions using the above-mentioned solvents. Results: The phenol and flavonoid content was found to be maximum in the methanol extracts. All the extracts and fractions showed significant levels of antioxidant activity except hexane extract. Conclusion: The tubers of H. pectinata were found to possess a significant antioxidant potential and can be explored further for isolation and preclinical investigation for the ailment of various diseased states and disorders.

scholarly journals Phytochemical composition and in vitro antioxidant activity of methanolic and aqueous extracts of aerial part of Pentatropis nivalis(Asclepiadaceae)

2018 ◽  
Vol 10 (1) ◽  
pp. 68
Author(s):  
Nilesh P. Babre ◽  
T. Shivraj Gouda ◽  
Narayanswamy Lachmanan Gowrishankar
Keyword(s):  
Antioxidant Activity ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Antioxidant Potential ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Phytochemical Composition ◽  
Solvent Systems ◽  
In Vitro Antioxidant Activity

<p>The objective of present study was to evaluate preliminary phytoconstitutents and <em>invitro</em> antioxidant potential of <em>Pentatropis nivalis </em>(Asclepiadaceae). During the preliminary phytochemical analysis, methanolic and aqueous extracts of aerial part of <em>Pentatropis nivalis</em> was screened for the presence of phenolic, saponins, flavonoids, alkaloids, tannins and phytosterols. TLC of extracts were performed by using various solvent systems. Phytochemicals screening and TLC spots of MEPN and AEPN showed the presence of glycoside, steroids, terpenoids, phenolic, saponins, Methanolic extract showed better qualitative tests for presence of secondary metabolites than aqueous extract.The in-vitro antioxidant potential of extracts were evaluated by DPPH and FRAP, and both methods showed that the plant possesses good antioxidant activity.</p>

scholarly journals POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

2021 ◽  
Vol 45 ◽  
Author(s):  
Andréa Michel Sobottka ◽  
Elisandra Tessaro ◽  
Suelen Maier da Silva ◽  
Marina Pedron ◽  
Lara Tortini Seffrin
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Skin Diseases ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Butanol Fraction ◽  
Degraded Ecosystems

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

Phytol And Glycerol Derivatives From The Marine Green Alga Codium Iyengarii Of The Karachi Coast (Arabian Sea)

2001 ◽  
Vol 56 (8) ◽  
pp. 837-841 ◽  
Author(s):  
Muhammad Shaiq Ali ◽  
Muhammad Saleem ◽  
Viqar Uddin Ahmad ◽  
Simin Shameel
Keyword(s):  
Ethyl Acetate ◽  
Acid Hydrolysis ◽  
Green Alga ◽  
Arabian Sea ◽  
Methanolic Extract ◽  
Derivatives Of ◽  
Soluble Part ◽  
Marine Green Alga

The ethyl acetate soluble part of a methanolic extract of the marine green alga Codium iyengarii, collected from the Karachi coast of the Arabian Sea, afforded four new derivatives of glycerol named : codioside A,B,C,D (1-4), a new derivative of frans-phytol named codioester (5) and rrans-phytol (6). The structures of 1 -5 were elucidated with the aid of acid hydrolysis, methylation, GC-MS, and spectroscopy. The structure of 1 was confirmed through the HMBC NMR technique and the structures of compounds 2 -4 were based on the assignments made for 1

Evaluation of Cytotoxic Compounds from Calligonum comosum L. Growing in Egypt

2007 ◽  
Vol 62 (9-10) ◽  
pp. 656-660 ◽  
Author(s):  
Farid A. Badria ◽  
Madiha Ameen ◽  
Mohamed R. Akl
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methylene Chloride ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Desert Plant ◽  
Ehrlich Ascites ◽  
Cytotoxic Compounds ◽  
Egyptian Desert

Calligonum comosum (Polygonaceae), an Egyptian desert plant, was extracted and fractionated using petroleum ether, methylene chloride, and ethyl acetate. The total methanolic extract and other fractions were tested for their anticancer activity using Ehrlich ascites, brine shrimp and antioxidant assays. Ethyl acetate fraction proved to be the most active in all assays. Eight compounds were isolated, purified, and identified from this fraction as (+)- catechin (1), dehydrodicatechin A (2), kaempferol-3-O-rhamnopyranoside (3), quercitrin (quercetin-3-O-rhamnopyranoside) (4), β-sitosterol-3-O-glucoside (5), isoquercitrin (quercetin- 3-O-glucopyranoside) (6), kaempferol-3-O-glucuronide (7), and mequilianin (quercetin-3- O-glucuronide) (8). All isolated compounds were tested for their cytotoxicity and antioxidant activity. Compound 2 showed the best cytotoxic and antioxidant activity.

scholarly journals An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis

Antioxidants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 110
Author(s):  
Stefano Dall’Acqua ◽  
Stefania Sut ◽  
Kouadio Ibrahime Sinan ◽  
Gokhan Zengin ◽  
Irene Ferrarese ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Data Analysis ◽  
Multivariate Data Analysis ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Multivariate Data ◽  
Chemical Data ◽  
Acetylcholinesterase Inhibition ◽  
Biological Potential

Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were analyzed combining integrated NMR, LC-DAD-MSn, and LC-QTOF methods. In vitro antioxidant and enzyme inhibitory activities were evaluated, and the results were combined with chemical data using multivariate approaches. Phenolic acids, flavonoids, ellagitannins, and coumarins were identified and quantified in the extracts of aerial part and roots. Methanolic extract of S. hedysaroides aerial part showed the highest phenolic content and the highest antioxidant activity and cupric ion reducing antioxidant capacity. Dichloromethane extract of S. hedysaroides roots showed the highest inhibition of butyryl cholinesterase, while methanolic extract of S. hedysaroides aerial part was the most active tyrosinase inhibitor. Multivariate data analysis allowed us to observe a good correlation between phenolic compounds, especially caffeoylquinic derivatives and flavonoids and the antioxidant activity of extracts. Acetylcholinesterase inhibition was correlated with the presence of caffeoylquinic acids and coumarins. Overall, the present study appraised the biological potential of understudied S. hedysaroides, and provided a comprehensive approach combining metabolomic characterization of plant material and multivariate data analysis for the correlation of chemical data with results from multi-target biological assays.

scholarly journals Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn. (Skeel) Seed

2017 ◽  
Vol 14 (1) ◽  
pp. 9 ◽  
Author(s):  
Rohadi Rohadi ◽  
Umar Santoso ◽  
Sri Raharjo ◽  
Iip Izul Falah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Polar Fraction ◽  
Acid Oxidation ◽  
Syzygium Cumini ◽  
Compound Identification ◽  
Antioxidant Power

Methanolic extract of Java Plum (Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed (Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identification using Thin Layer Chromatography (TLC) showed that all fractions (polar, semi polar and hydrolyzed semi polar fraction) contained Gallic acid, Tannic acid and flavonol’s Rutin. HPLC-DAD analysis showed that its polar fraction contained 25 ppm flavonol’s Quercetine and 55181 ppm flavonol’s (+)- Catechin, ethyl acetate fraction contained 54 ppm flavonol’s Rutin and 528 ppm (+)- Catechine, while hydrolyzed ethyl acetate fraction contained 404 ppm Rutin and 28692 ppm (+)- Catechine.

scholarly journals Study on the Chemical Composition, Enzyme Inhibition and Antioxidant Activity of Ziziphora taurica subsp. cleonioides

2019 ◽  
Vol 9 (24) ◽  
pp. 5515 ◽  
Author(s):  
Cengiz Sarikurkcu ◽  
Eleni Kakouri ◽  
Rifat Tayyib Sarikurkcu ◽  
Petros A. Tarantilis
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Enzyme Inhibition ◽  
Methanolic Extract ◽  
Skin Pigmentation ◽  
Extraction Process ◽  
Total Phenolic ◽  
Chemical Profile ◽  
Flavonoid Content ◽  
And Oxidative Stress

Ziziphora is a plant used in Turkish and Iran traditional medicine for its antibacterial activity, sedative and stomach soothing properties. Although the chemical profile of the essential oil of different Ziziphora species is well documented, data regarding plant extracts are incomplete. In this study extracts from Ziziphora taurica subsp. cleonioides were obtained using ethyl acetate, methanol and water and the chemical profile of the aerial part of the plant was elucidated. Among the compounds identified, rosmarinic acid was the most abundant (3375.67 ± 38.02 μg/mL), at the extract of methanol, followed by chlorogenic acid (3225.10 ± 16.44). Enzyme inhibition activity against α-amylase and tyrosinase was also estimated. The ethyl acetate extract showed the highest α-amylase activity (1.95 ± 0.04 mg/mL), while the best anti-tyrosinase activity was calculated for the methanolic extract (1.25 ± 0.01 mg/mL). In addition, total phenolic, flavonoid content and antioxidant activity were evaluated. According to our results, bioactivity of the plant is of great interest, nonetheless, at the same time, it is strongly depended on the solvent used during the extraction process. Our data suggest that the plant under study may be an important source to consider against metabolic, skin pigmentation and oxidative stress related disorders.

scholarly journals IN VITRO ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ERANTHEMUM CAPENSE

2019 ◽  
pp. 33-35
Author(s):  
ANOOPA JOHN L ◽  
KANNAPPAN N ◽  
MANOJKUMAR P
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Ethyl Acetate Extract ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Gram Negative ◽  
Anti Inflammatory

Objective: The present study was aimed to rationalize the scientific basis in traditional use of Eranthemum capense as an antibacterial, antifungal, and anti-inflammatory agent. Methods: Agar well diffusion method is widely used to evaluate the antimicrobial activity of the E. capense aerial part of methanolic and ethyl acetate plant extracts. The same amount (15–20 mL) of Mueller-Hinton agar was poured on glass Petri plates of same size and allowed to solidify. E. capense aerial part of methanolic and ethyl acetate extracts was evaluated in vitro for their anti-inflammatory activities using the bovine serum albumin protein denaturation assay. Results: The result of the study shows that methanolic exract (T3) of the plant, E. capense shows 16 mm zone of inhibition against Pseudomonas fluorescens, while the ethyl acetate extract of the same plant shows 14 mm zone of inhibition against P. fluorescens and E. coli. Hence the methanolic extract of T3 sample shows the antibacterial activity against gram negative bacteria, where as the ethyl acetate extract of T3 shows antibacterial activity against both gram positive and gram negative bacteria. The experimental report revealed that, the methanolic and ethyl acetate extract of the same plant produces zero percentage zone of inhibition against Aspergillus niger and Mucor, hence it does not show any antifungal activity. Conclusion: It is observed that the EA and methanolic extract of E. capense can be used in the treatment of inflammation due to the significant percentage of inhibition of protein denaturation as well as its prove the good antimicrobial agent.

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