Lipidomic study of olive fruit and oil using TiO2 nanoparticle based matrix solid-phase dispersion and MALDI-TOF/MS

Isobaric arylureido peptoids undergo SS1 and SS2 fragmentations in MALDI-TOF MS/MS, thus offering a sequencing mechanism for arylureides without molecular encoding.

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Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Journal of Analytical Methods in Chemistry ◽

10.1155/2018/7832987 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Hoa Thi Le ◽

Kyu H. Park ◽

Woong Jung ◽

Hyung Soon Park ◽

Tae Woo Kim

Keyword(s):

Ionic Liquid ◽

Human Serum ◽

Solid Phase ◽

Liquid Matrix ◽

Dihydroxybenzoic Acid ◽

Established Method ◽

Maldi Tof Ms ◽

Microwave Assisted ◽

Maldi Tof ◽

Tof Ms

We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS. The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range. Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans. However, with the established method, i.e., DHB/An method, only 15 glycans were assigned. To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.

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Analysis of O-and N-linked glycopeptide libraries by MALDI-TOF MS: Application in solid phase assays of carbohydrate-binding-proteins

Peptides Frontiers of Peptide Science ◽

10.1007/0-306-46862-x_12 ◽

2002 ◽

pp. 45-46

Author(s):

Phaedria M. St. Hilaire ◽

Morten Meldal ◽

Klaus Bock

Keyword(s):

Binding Proteins ◽

Solid Phase ◽

Carbohydrate Binding ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Carbohydrate Binding Proteins ◽

Tof Ms

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Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

Molecules ◽

10.3390/molecules24122311 ◽

2019 ◽

Vol 24 (12) ◽

pp. 2311

Author(s):

Rosa Terracciano ◽

Mariaimmacolata Preianò ◽

Giuseppina Maggisano ◽

Corrado Pelaia ◽

Rocco Savino

Keyword(s):

Synovial Fluid ◽

Mesoporous Silica ◽

Sample Preparation ◽

Solid Phase ◽

Phase Extraction ◽

Dispersive Solid Phase Extraction ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Versatile Tool ◽

Tof Ms

Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

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