Effects of dietary vitamin D3 administration on innate immune parameters of seabream (Sparus aurata L.)

The humoral innate immune response of gilthead seabream (Sparus aurataL.) against the myxozoanEnteromyxum leeihas been studied. At 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mRNA expression of pro-inflammatory cytokines (IL-1β and TNFα) in head-kidney. The parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62·5%) at the last sampling time (108 days). Recipient fish started to die after 74 days of cohabitation. In general, alternative complement activity was higher whereas the peroxidase level was lower in recipient fish than in controls. Moreover, IL-1β mRNA expression increased while the TNFα gene expression decreased in recipient fish. These data demonstrate the involvement of complement activity in the defence mechanisms of the gilthead seabream against the myxosporeanE. leei. Within the recipient fish group, few differences were observed in the studied immune parameters betweenE. leei-parasitized and non-parasitized recipient fish. Parasitological and immunological implications ofE. leeiinfections in Mediterranean fish farms are discussed.

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Effects of dietary inulin and heat-inactivated Bacillus subtilis on gilthead seabream (Sparus aurata L.) innate immune parameters

Beneficial Microbes ◽

10.3920/bm2011.0028 ◽

2012 ◽

Vol 3 (1) ◽

pp. 77-81 ◽

Cited By ~ 13

Author(s):

R. Cerezuela ◽

A. Cuesta ◽

J. Meseguer ◽

M. Esteban

Keyword(s):

Bacillus Subtilis ◽

Sparus Aurata ◽

Innate Immune ◽

Gilthead Seabream ◽

Cytotoxic Activities ◽

Feeding Trial ◽

Complement Activity ◽

Immune Parameters ◽

Group B ◽

Dietary Treatments

In the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated Bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (Sparus aurata). Forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), B. subtilis (107 cfu/g, probiotic group), or B. subtilis + inulin (107 cfu/g + 10 g/kg, synbiotic group). After two and four weeks, six fish of each group were sampled, with the main innate immune parameters (natural haemolytic complement activity, serum and leucocyte peroxidase, phagocytosis, respiratory burst, and cytotoxic activities) being determined. Inulin or heat-inactivated B. subtilis failed to significantly stimulate the innate immune parameters assayed, although some activities showed no significant increase through these treatments. A combination of inulin and B. subtilis resulted in an increase of such parameters, with the haemolytic complement activity being the only one significantly stimulated. To conclude, inulin and B. subtilis, when administered as a synbiotic, have a synergistic effect and enhance some innate immune parameters of gilthead seabream.

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