Dietary vitamin D3 improves postprandial hyperglycemia in aged mice

Background: Vitamin D and calcium are important dietary compounds that affect bone mass, even if other minerals (potassium, zinc, etc.) and vitamins (A, C and K) are also involved. Vitamin D and certain minerals, in fact, play an important role in calcium homeostasis and calcium absorption. Hip fracture incidence is higher in Europe and the United States, where calcium is frequently included in the human diet; while the occurrence of these fractures is lower in developing countries, where diets are often poor in calcium. This condition is named the “calcium paradox”, and may be partially explained by phosphate toxicity, which can negatively affect mineral metabolism. It is important to maintain correct dietary calcium-phosphate balance in order to have a healthy life, reducing the risk of osteoporotic fractures in older people. Vitamin D can also act as a hormone; vitamin D2 (ergocalciferol) is derived from the UV-B radiation of ergosterol, the natural vitamin D precursor detected in plants, fungi, and invertebrates. Vitamin D3 (cholecalciferol) is synthesized by sunlight exposure from 7-dehydrocholesterol, a precursor of cholesterol that can also act as provitamin D3. Dietary intake of vitamin D3 is essential when the skin is exposed for short periods to ultraviolet B light (UV-B), a category of invisible light rays such as UV-A and UV-C. This can be considered the usual situation in northern latitudes during the winter season, or the typical lifestyle for older people and/or for people with very white delicate skin. The actual recommended daily intake of dietary vitamin D is strictly correlated with age, ranging from 5 μg for infants, children, teenagers, and adults—including pregnant and lactating women—to 15 μg for people over 65 years.

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Impact of dietary vitamin D3 supplements in nursery diets on subsequent growth and bone responses of pigs during an immune challenge

Journal of Animal Science ◽

10.1093/jas/skz347 ◽

2019 ◽

Vol 97 (12) ◽

pp. 4895-4903 ◽

Cited By ~ 1

Author(s):

Morgan McCue ◽

Jamie L Reichert ◽

Thomas D Crenshaw

Keyword(s):

Vitamin D3 ◽

Growing Pigs ◽

Whole Body ◽

Dietary Vitamin ◽

Vitamin D Metabolites ◽

Immune Challenge ◽

Subsequent Growth ◽

Limited Evidence ◽

Beneficial Effects ◽

Nutrient Supplements

Abstract Limited evidence is available to validate beneficial responses from extra nutrient supplements for mediation of growth suppression that results from immune challenges. Extrarenal roles of vitamin D metabolites in immune function implicate vitamin D3 supplements as a nutrient for potential beneficial effects. The current objective was to assess growth and bone ash responses to dietary vitamin D3 (D) supplements for growing pigs undergoing an immune challenge. At weaning, 216 crossbred pigs (4 pigs/pen, 6 pens/treatment) were randomly allotted within sex and weight blocks to 1 of the 9 treatments. Treatments included D supplements (0, 100, or 800 IU/kg) in a factorial arrangement with 3 vaccine (V) protocols; no injection (0 × V), a single 2 mL injection of a Lawsonia intracellularis vaccine at day 14 (1 × V), or 2 mL injections of the same vaccine at days 0 and 7 (2 × V). An adjustment diet with no supplemental D was fed for 1 wk, then assigned D diets for 2 wk (P2). After P2, all pigs were phase-fed standard diets (D = 280 IU/kg) to assess subsequent growth to 115 kg. No differences due to D supplements or vaccination protocol were detected in ADG (0.233 ± 0.021 kg/d) or GF (0.642 ± 0.028 kg/d) over the 21-d nursery trial; however, ADFI was lower (P < 0.10) in pigs fed D levels of 0 vs. 100 and 800 (0.340 vs. 0.375, 0.372 ± 0.027 kg/d). Bone mineral content (g) from whole-body dual energy X-ray absorptiometry scans at 9 wk (n = 4 pigs/treatment) was lower in pigs fed 0 vs. 100 and 800 IU of D (287 vs. 325, 323 ± 34.1 g/pig). Growth from nursery to 115 kg was lower (P < 0.01) in pigs fed D levels of 0 vs.100 and 800 (0.828 vs. 0.876, 0.889 ± 0.021 kg/d). At market, approximately two-thirds of pigs showed positive L. intracellularis serology titers regardless of treatment. Limited evidence for D-mediation of an immune challenge using the vaccination protocols may be a consequence of limited vaccine effects on growth in the nursery and seroconversion of most pigs to L. intracellularis by market.

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The effect of dietary vitamin D3, minerals (Ca, P) and plant-protein sources in the development of systemic granulomatosis in meagre (Argyrosomus regius, Asso, 1801)

Aquaculture ◽

10.1016/j.aquaculture.2020.735052 ◽

2020 ◽

Vol 521 ◽

pp. 735052

Author(s):

M.I. Tsertou ◽

S. Chatzifotis ◽

R. Fontanillas ◽

E. Cotou ◽

E. Fountoulaki ◽

...

Keyword(s):

Vitamin D3 ◽

Plant Protein ◽

Dietary Vitamin ◽

Protein Sources ◽

Plant Protein Sources ◽

Argyrosomus Regius

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Tissue Distribution of Cholecalciferol and 25-hydroxycholecalciferol in Normal and Obese Mice Fed Different Levels of Vitamin D (P24-003-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz044.p24-003-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Chan Yoon Park ◽

Yongho Shin ◽

Jeong-Han Kim ◽

Sung Nim Han

Keyword(s):

Vitamin D ◽

Adipose Tissue ◽

Vitamin D3 ◽

Control Level ◽

Dietary Vitamin ◽

Epididymal Adipose Tissue ◽

Mrna Levels ◽

Lower Serum ◽

Vitamin D Intake ◽

Different Levels

Abstract Objectives Vitamin D deficiency is often observed in obese person. One of the mechanisms suggested is the decreased bioavailability of vitamin D due to its deposition in adipose tissue. We investigated the effects of obesity on vitamin D distribution by measuring 25(OH)D levels in circulation and comparing vitamin D content in liver and adipose tissue from obese and control mice fed different levels of vitamin D. Methods Six-wk-old C57BL/6 mice were fed control or high fat (10 or 45% kcal fat, CON or HFD) diets containing different levels of vitamin D3 (1000, or 25,000 IU/kg of diet, CVd or HVd) for 13 wks. Serum 25-hydroxyvitamin D (25(OH)D) level was determined by radioimmunoassay. Vitamin D3 and 25(OH)D3 levels in the liver and epididymal adipose tissue (AT) were quantified by LC-MS/MS. mRNA levels of liver Cyp2r1 and Cyp27a1 were determined by real-time PCR. Results Overall, serum 25(OH)D levels were significantly higher in the HVd groups compared with CVd groups. There was no difference in serum 25(OH)D levels between CON-CVd and HFD-CVd groups. However, in the vitamin D supplemented groups, HFD-HVd group had significantly lower serum 25(OH)D levels (20% lower) than CON-HVd group. Vitamin D3 levels in the liver and AT were 55 and 100 times higher in the HVd groups (liver and AT: 719 and 318 ng/g tissue) compared with the CVd groups. 25(OH)D3 levels in the liver and AT were also 3.3 and 2.4 times higher in the HVd groups (liver and AT: 33.9 and 18.9 ng/g tissue) than those of the CVd groups. Total amount of vitamin D3 in the liver and AT were significantly higher in the HFD-HVd group (121 and 44% higher) compared with CON-HVd group. However, when mice were fed the control levels of vitamin D, dietary fat levels did not affect the vitamin D3 amount in the liver and AT. Liver Cyp2r1 and Cyp27a1 mRNA levels did not differ among groups. Conclusions When vitamin D intake was at a supplementation level, a significant amount of dietary vitamin D seemed to be stored in the liver and AT; thus excess body adiposity could contribute to lower serum 25(OH)D level. However, at a control level of vitamin D intake, obesity did not affect tissue vitamin D amount and serum 25(OH)D levels. Funding Sources Supported by the grant from the National Research Foundation (NRF) of Korea (NRF-2018R1D1A1B070491) and Research Grant from Research Affairs at Seoul National University.

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