Effects of dietary inulin and heat-inactivated Bacillus subtilis on gilthead seabream (Sparus aurata L.) innate immune parameters

2012 ◽  
Vol 3 (1) ◽  
pp. 77-81 ◽  
Author(s):  
R. Cerezuela ◽  
A. Cuesta ◽  
J. Meseguer ◽  
M. Esteban
Keyword(s):  
Bacillus Subtilis ◽  
Sparus Aurata ◽  
Innate Immune ◽  
Gilthead Seabream ◽  
Cytotoxic Activities ◽  
Feeding Trial ◽  
Complement Activity ◽  
Immune Parameters ◽  
Group B ◽  
Dietary Treatments

In the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated Bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (Sparus aurata). Forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), B. subtilis (107 cfu/g, probiotic group), or B. subtilis + inulin (107 cfu/g + 10 g/kg, synbiotic group). After two and four weeks, six fish of each group were sampled, with the main innate immune parameters (natural haemolytic complement activity, serum and leucocyte peroxidase, phagocytosis, respiratory burst, and cytotoxic activities) being determined. Inulin or heat-inactivated B. subtilis failed to significantly stimulate the innate immune parameters assayed, although some activities showed no significant increase through these treatments. A combination of inulin and B. subtilis resulted in an increase of such parameters, with the haemolytic complement activity being the only one significantly stimulated. To conclude, inulin and B. subtilis, when administered as a synbiotic, have a synergistic effect and enhance some innate immune parameters of gilthead seabream.

Gilthead seabream (Sparus aurataL.) innate defence against the parasiteEnteromyxum leei(Myxozoa)

Parasitology ◽  
2005 ◽  
Vol 132 (1) ◽  
pp. 95-104 ◽  
Author(s):  
A. CUESTA ◽  
P. MUÑOZ ◽  
A. RODRÍGUEZ ◽  
I. SALINAS ◽  
A. SITJÀ-BOBADILLA ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Sparus Aurata ◽  
Head Kidney ◽  
Innate Immune ◽  
Gilthead Seabream ◽  
Fish Farms ◽  
Complement Activity ◽  
Immune Parameters ◽  
Fish Group ◽  
Innate Defence

The humoral innate immune response of gilthead seabream (Sparus aurataL.) against the myxozoanEnteromyxum leeihas been studied. At 10, 22, 38, 52 and 108 days of cohabitation fish were sampled to examine gut histology and to determine serum innate immune parameters and the mRNA expression of pro-inflammatory cytokines (IL-1β and TNFα) in head-kidney. The parasite was successfully transmitted to 45% of the recipient fish and prevalence reached a maximum (62·5%) at the last sampling time (108 days). Recipient fish started to die after 74 days of cohabitation. In general, alternative complement activity was higher whereas the peroxidase level was lower in recipient fish than in controls. Moreover, IL-1β mRNA expression increased while the TNFα gene expression decreased in recipient fish. These data demonstrate the involvement of complement activity in the defence mechanisms of the gilthead seabream against the myxosporeanE. leei. Within the recipient fish group, few differences were observed in the studied immune parameters betweenE. leei-parasitized and non-parasitized recipient fish. Parasitological and immunological implications ofE. leeiinfections in Mediterranean fish farms are discussed.

Effects of inulin on gilthead seabream (Sparus aurata L.) innate immune parameters

2008 ◽  
Vol 24 (5) ◽  
pp. 663-668 ◽  
Author(s):  
R CEREZUELA ◽  
A CUESTA ◽  
J MESEGUER ◽  
M ANGELESESTEBAN
Keyword(s):  
Sparus Aurata ◽  
Innate Immune ◽  
Gilthead Seabream ◽  
Immune Parameters

Humoral immune parameters in serum of gilthead seabream (Sparus aurata L.) after induced skin injury

2018 ◽  
Vol 75 ◽  
pp. 291-294 ◽  
Author(s):  
Diana Ceballos-Francisco ◽  
Francisco A. Guardiola ◽  
Héctor Cordero ◽  
Alberto Cuesta ◽  
M. Ángeles Esteban
Keyword(s):  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Skin Injury ◽  
Humoral Immune ◽  
Immune Parameters

Effects of dietary vitamin D3 administration on innate immune parameters of seabream (Sparus aurata L.)

2009 ◽  
Vol 26 (2) ◽  
pp. 243-248 ◽  
Author(s):  
Rebeca Cerezuela ◽  
Alberto Cuesta ◽  
José Meseguer ◽  
M Ángeles Esteban
Keyword(s):  
Vitamin D3 ◽  
Sparus Aurata ◽  
Innate Immune ◽  
Dietary Vitamin ◽  
Immune Parameters

scholarly journals Immune Status and Hepatic Antioxidant Capacity of Gilthead Seabream Sparus aurata Juveniles Fed Yeast and Microalga Derived β-glucans

Marine Drugs ◽  
2021 ◽  
Vol 19 (12) ◽  
pp. 653
Author(s):  
Bruno Reis ◽  
Ana Teresa Gonçalves ◽  
Paulo Santos ◽  
Manuel Sardinha ◽  
Luís E. C. Conceição ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Status ◽  
Sparus Aurata ◽  
Dietary Treatment ◽  
Gilthead Seabream ◽  
Oxidative Stress Biomarkers ◽  
Yeast Saccharomyces Cerevisiae ◽  
Commercial Diet ◽  
Immune Parameters ◽  
Dietary Concentration

This work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects.

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