Comparative analysis of the expression patterns of eight suppressors of cytokine signaling in tongue sole, Cynoglossus semilaevis

Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.

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Fish Suppressors of Cytokine Signaling (SOCS): Gene Discovery, Modulation of Expression and Function

Journal of Signal Transduction ◽

10.1155/2011/905813 ◽

2011 ◽

Vol 2011 ◽

pp. 1-20 ◽

Cited By ~ 32

Author(s):

Tiehui Wang ◽

Bartolomeo Gorgoglione ◽

Tanja Maehr ◽

Jason W. Holland ◽

Jose L. González Vecino ◽

...

Keyword(s):

Genome Duplication ◽

Expression Patterns ◽

Duplication Event ◽

Poly I:C ◽

Cytokine Signaling ◽

Parasitic Infections ◽

Genome Duplication Event ◽

Specific Expression ◽

Functional Studies ◽

Suppressors Of Cytokine Signaling

The intracellular suppressors of cytokine signaling (SOCS) family members, including CISH and SOCS1 to 7 in mammals, are important regulators of cytokine signaling pathways. So far, the orthologues of all the eight mammalian SOCS members have been identified in fish, with several of them having multiple copies. Whilst fish CISH, SOCS3, and SOCS5 paralogues are possibly the result of the fish-specific whole genome duplication event, gene duplication or lineage-specific genome duplication may also contribute to some paralogues, as with the three trout SOCS2s and three zebrafish SOCS5s. Fish SOCS genes are broadly expressed and also show species-specific expression patterns. They can be upregulated by cytokines, such as IFN-γ, TNF-α, IL-1β, IL-6, and IL-21, by immune stimulants such as LPS, poly I:C, and PMA, as well as by viral, bacterial, and parasitic infections in member- and species-dependent manners. Initial functional studies demonstrate conserved mechanisms of fish SOCS action via JAK/STAT pathways.

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Transcriptome Profiling Reveals the Sexual Dimorphism of Gene Expression Patterns during Gonad Differentiation in the Half-Smooth Tongue Sole (Cynoglossus semilaevis)

Marine Biotechnology ◽

10.1007/s10126-020-09996-x ◽

2020 ◽

Author(s):

Genmei Lin ◽

Dong Gao ◽

Jianguo Lu ◽

Xiaowen Sun

Keyword(s):

Gene Expression ◽

Sexual Dimorphism ◽

Expression Patterns ◽

Transcriptome Profiling ◽

Cynoglossus Semilaevis ◽

Gene Expression Patterns ◽

Tongue Sole ◽

Gonad Differentiation ◽

Half Smooth Tongue Sole

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Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis

Biology ◽

10.3390/biology10090830 ◽

2021 ◽

Vol 10 (9) ◽

pp. 830

Author(s):

Jing Li ◽

Bao Shi ◽

Chongnv Wang ◽

Changwei Shao ◽

Xuezhou Liu ◽

...

Keyword(s):

Ovarian Development ◽

Developmental Stages ◽

Expression Patterns ◽

Ring Structure ◽

Cynoglossus Semilaevis ◽

Biological Processes ◽

Rnase R ◽

Specific Expression ◽

Qrt Pcr ◽

Tongue Sole

CircRNAs are novel endogenous non-coding small RNAs involved in the regulation of multiple biological processes. However, little is known regarding circRNAs in ovarian development and maturation of fish. Our study, for the first time, provides the genome-wide overview of the types and relative abundances of circRNAs in tongue sole tissues during three ovarian developmental stages. We detected 6790 circRNAs in the brain, 5712 in the pituitary gland, 4937 in the ovary and 4160 in the liver. Some circRNAs exhibit tissue-specific expression, and qRT-PCR largely confirmed 6 differentially expressed (DE) circRNAs. Gene Ontology and KEGG pathway analyses of DE mRNAs were performed. Some DE circRNA parental genes were closely associated with biological processes in key signalling pathways and may play essential roles in ovarian development and maturation. We found that the selected circRNAs were involved in 10 pathways. RNase R digestion experiment and Sanger sequencing verified that the circRNA had a ring structure and was RNase R resistant. qRT-PCR results largely confirmed differential circRNA expression patterns from the RNA-seq data. These findings indicate that circRNAs are widespread in terms of present in production-related tissues of tongue sole with potentially important regulatory roles in ovarian development and maturation.

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