Transcriptome Profiling Reveals the Sexual Dimorphism of Gene Expression Patterns during Gonad Differentiation in the Half-Smooth Tongue Sole (Cynoglossus semilaevis)

2020 ◽  
Author(s):  
Genmei Lin ◽  
Dong Gao ◽  
Jianguo Lu ◽  
Xiaowen Sun
Keyword(s):  
Gene Expression ◽  
Sexual Dimorphism ◽  
Expression Patterns ◽  
Transcriptome Profiling ◽  
Cynoglossus Semilaevis ◽  
Gene Expression Patterns ◽  
Tongue Sole ◽  
Gonad Differentiation ◽  
Half Smooth Tongue Sole

scholarly journals Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis

2021 ◽  
Vol 12 ◽  
Author(s):  
Yani Dong ◽  
Likang Lyu ◽  
Haishen Wen ◽  
Bao Shi
Keyword(s):  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Interaction ◽  
Cynoglossus Semilaevis ◽  
Rna Seq ◽  
Tongue Sole ◽  
Gene Interaction Networks ◽  
Half Smooth Tongue Sole ◽  
The Brain

Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.

scholarly journals Transvection regulates the sex-biased expression of a fly X-linked gene

Science ◽  
2021 ◽  
Vol 371 (6527) ◽  
pp. 396-400 ◽  
Author(s):  
Charalampos Chrysovalantis Galouzis ◽  
Benjamin Prud’homme
Keyword(s):  
Gene Expression ◽  
Sexual Dimorphism ◽  
Sex Determination ◽  
Expression Pattern ◽  
X Chromosome ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Regulatory Interaction ◽  
Linked Gene ◽  
Homologous Chromosomes

Sexual dimorphism in animals results from sex-biased gene expression patterns. These patterns are controlled by genetic sex determination hierarchies that establish the sex of an individual. Here we show that the male-biased wing expression pattern of the Drosophila biarmipes gene yellow, located on the X chromosome, is independent of the fly sex determination hierarchy. Instead, we find that a regulatory interaction between yellow alleles on homologous chromosomes (a process known as transvection) silences the activity of a yellow enhancer functioning in the wing. Therefore, this enhancer can be active in males (XY) but not in females (XX). This transvection-dependent enhancer silencing requires the yellow intron and the chromatin architecture protein Mod(mdg4). Our results suggest that transvection can contribute more generally to the sex-biased expression of X-linked genes.

scholarly journals The effect of dietary arachidonic acid (ARA) on growth performance, fatty acid composition and expression of ARA metabolism-related genes in larval half-smooth tongue sole (Cynoglossus semilaevis)

2015 ◽  
Vol 113 (10) ◽  
pp. 1518-1530 ◽  
Author(s):  
Yuhui Yuan ◽  
Songlin Li ◽  
Kangsen Mai ◽  
Wei Xu ◽  
Yanjiao Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arachidonic Acid ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Growth Performance ◽  
Acid Composition ◽  
Cynoglossus Semilaevis ◽  
Tongue Sole ◽  
Cox 2 ◽  
Half Smooth Tongue Sole

The present study was conducted to investigate the effects of dietary arachidonic acid (ARA) on growth performance, fatty acid composition and ARA metabolism-related gene expression in larval half-smooth tongue sole (Cynoglossus semilaevis). Larvae (35 d after hatching, 54 (sem 1) mg) were fed diets with graded concentrations of ARA (0·01, 0·39, 0·70, 1·07, 1·42 and 2·86 % dry weight) five times per d to apparent satiation for 30 d. Results showed that increased dietary ARA concentration caused a significant non-linear rise to a plateau in survival rate, final body weight and thermal growth coefficient, and the maximum values occurred with the 1·42 % ARA treatment. As dietary ARA increased to 1·07 or 1·42 %, activities of trypsin, leucine aminopeptidase and alkaline phosphatase levels increased, but they decreased with higher ARA concentrations. The fatty acid composition of tongue sole larvae was almost well correlated with their dietary fatty acid profiles, and the EPA content of the larvae decreased with increasing dietary ARA. Meanwhile, the partial sequences of COX-1a (cyclo-oxygenase-1a), COX-1b (cyclo-oxygenase-1b), COX-2 (cyclo-oxygenase-2), 5-LOX (5-lipoxygenase) and CYP2J6-like (cytochrome P450 2J6-like) were also obtained. Both COX-2 and 5-LOX mRNA expression levels significantly increased to a plateau in an ‘L’-shaped manner as dietary ARA increased to 1·07 or 1·42 %, but no significant differences were found in the gene expression of COX-1a, COX-1b or CYP2J6-like. These results suggest that 1·07–1·42 % dietary ARA was beneficial to the growth performance of larval tongue sole, and the regulation of dietary ARA on the growth performance of larvae was probably involved in altering the mRNA expression of COX-2 and 5-LOX.

scholarly journals Transcriptome profiling revealed diverse gene expression patterns in poplar (Populus × euramericana) under different planting densities

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0217066
Author(s):  
Kun Ning ◽  
Changjun Ding ◽  
Qinjun Huang ◽  
Weixi Zhang ◽  
Chengchao Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Transcriptome Profiling ◽  
Gene Expression Patterns ◽  
Populus Euramericana
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