Immuno-protective efficiency of feed-based whole-cell inactivated bivalent vaccine against Streptococcus and Aeromonas infections in red hybrid tilapia (Oreochromis niloticus × Oreochromis mossambicus)

Author(s):  
Md Shirajum Monir ◽  
Md Sabri Mohd Yusoff ◽  
Zarirah Mohamed Zulperi ◽  
Hasliza Abu Hassim ◽  
Mohd Zamri-Saad ◽  
...  
2020 ◽  
Vol 3 (2) ◽  
pp. 92-101
Author(s):  
Indra Topik Maulana ◽  
Rizka Wulan Sari ◽  
Rinda Sri Partina ◽  
Isnaeni Nur Azizah

Penelitian terkait telaah kandungan asam lemak esensial dari empat jenis minyak ikan konsumsi di Jawa Barat telah selesai dilakukan. Penelitian ini bertujuan untuk memperoleh data ilmiah terkait kandungan asam lemak esensial didalam empat jenis ikan konsumsi. Bahan ikan yang digunakan adalah ikan mujair (Oreochromis mossambicus), ikan nila (Oreochromis niloticus), ikan gurami (Osphronemus goramy), dan ikan Bandeng (Chanos chanos). Minyak dari setiap jenis ikan diekstraksi dengan metode ekstraksi sinambung. Selanjutnya minyak ditransesterifikasi menjadi FAME kemudian dianalisis dengan menggunakan Kromatografi Gas-Spektroskopi Massa. Data hasil penelitian menunjukkan ikan mujair memiliki rendemen paling tinggi yaitu 8,57 + 0,06 % dari total bahan kering tanpa kepala dan ekor.  Ikan nila mengandung minyak paling rendah yaitu 0,28 + 0,09 %. Berdasarkan penggolongan asam lemak, Minyak ikan mujaer mengandung SFA 35,36 + 4,86 %, MUFA 31,55 + 1,79 %, PUFA 19,15 + 2,05 %. Minyak ikan bandeng mengandung SFA 38,12 + 4,86 %, MUFA 36,64 + 2,21 % dan PUFA 18,4 + 2,02 %. Minyak Ikan gurami mengandung SFA 41,65 + 2,35   %, MUFA 40,29 + 1,13 %. Minyak Ikan Nila mengandung SFA 34,11 + 1,73%, MUFA  27,47  + 2,11 %, dan PUFA 38,43 + 2,81 %. Asam lemak esensial utama yang ditemukan pada penelitian ini diantaranya adalah ARA(ω-6), EPA(ω-3), dan DHA(ω-6).


Biotecnia ◽  
2020 ◽  
Vol 22 (2) ◽  
pp. 5-12
Author(s):  
Alejandro Mcdonal-Vera ◽  
Wilfrido M. Contreras-Sánchez ◽  
Neil J. Duncan ◽  
Kevin Fitzsimmons ◽  
Maria J. Contreras- García ◽  
...  

In the present investigation, we evaluated if the presence of tilapia affects growth and survival of shrimp during pre-growth and grow-out phases. In both experiments, we used a random-blocks design in a single earth pond (0.75 ha) that was prepared and divided into nine (7 x 30 m) enclosures that were considered to be three blocks of three enclosures each. Each randomly stocked block consisted on Pacific white shrimp Litopenaeus vannamei (30 shrimp/m2) and three different densities (0.0, 0.25 and 0.50 fish/m2) of red tilapia hybrids (Oreochromis mossambicus x Oreochromis niloticus) to give three different treatments with three replicates. The results from the pre-growth and grow-out experiments followed the same trend. In both cases, we found no significant statistical differences (P>0.05) in the final average weight of the shrimp. However, the experimental blocks had a significant (P<0.05) effect on the mean sample weight of shrimp. Shrimp in blocks B and C were significantly bigger than shrimp from block A (P<0.05). For both experiments, we found no effect of the presence of tilapias on survival or growth of shrimp.RESUMENEvaluamos si la presencia de tilapias libres afecta el crecimiento y la supervivencia de camarones en fase de preengorda y engorda. Para ambos experimentos, utilizamos un diseño de bloques al azar en un estanque rústico (0.75 ha) que fue preparado y dividido en nueve encierros (7 x 30 m), se formaron tres bloques con tres encierros cada uno. Cada encierro dentro de cada bloque fue aleatoriamente seleccionado para sembrar camarón blanco del Pacífico, Litopenaeus vannamei (30 camarones/m2) y tres diferentes densidades (0.0, 0.25 y 0.50 peces/m2) de híbrido de tilapia roja (Oreochromis mossambicus x Oreochromis niloticus) para formar tres tratamientos con tres réplicas. Los resultados del experimento de pre-engorda y engorda siguieron la misma tendencia. En ambos experimentos no encontramos diferencias estadísticamente en el crecimiento de los camarones ni en las tilapias. Sin embargo, los bloques experimentales presentaron diferencias significativas (P<0.05) en el peso promedio final. Los camarones de los bloques B y C fueron estadísticamente más grandes (P<0.05) que los del bloque A. Para las dos etapas de crecimiento de los camarones, no se observó un efecto de la presencia de tilapias sobre la sobrevivencia o crecimiento de los camarones.


2010 ◽  
Vol 55 (3) ◽  
Author(s):  
Adriana García-Vásquez ◽  
Haakon Hansen ◽  
Kevin Christison ◽  
Miquel Rubio-Godoy ◽  
James Bron ◽  
...  

AbstractGyrodactylus infections in intensively-reared populations of Nile tilapia, Oreochromis niloticus niloticus, have been associated world-wide with high mortalities of juvenile fish. In this study, 26 populations of Gyrodactylus parasitising either O. n. niloticus or Mozambique tilapia, Oreochromis mossambicus, were sampled from fourteen countries and compared with type material of Gyrodactylus cichlidarum Paperna, 1968, Gyrodactylus niloticus (syn. of G. cichlidarum) and Gyrodactylus shariffi Cone, Arthur et Bondad-Reantaso, 1995. Representative specimens from each population were bisected, each half being used for morphological and molecular analyses. Principal component analyses (PCA) identified five distinct clusters: (1) a cluster representing G. cichlidarum collected from O. n. niloticus from 13 countries; (2) the G. shariffi paratype; (3) three specimens with pronounced ventral bar processes collected from two populations of Mexican O. n. niloticus (Gyrodactylus sp. 1); (4) four specimens collected from an Ethiopian population nominally identified as O. n. niloticus (Gyrodactylus sp. 2); (5) nine gyrodactylids from South African O. mossambicus (Gyrodactylus sp. 3). Molecular analyses comparing the sequence of the ribosomal transcribed spacer regions (ITS 1 and 2) and the 5.8S gene from the non-hook bearing half of worms representative for each population and for each cluster of parasites, confirmed the presence of G. cichlidarum in most samples analysed. Molecular data also confirmed that the DNA sequence of Gyrodactylus sp. 2 and Gyrodactylus sp. 3 (the morphologically-cryptic group of South African specimens from O. mossambicus) differed from that of G. cichlidarum and therefore represent new species; no sequences were obtained from Gyrodactylus sp. 1. The current study demonstrates that G. cichlidarum is the dominant species infecting O. n. niloticus, being found in 13 of the 15 countries sampled.


Our Nature ◽  
1970 ◽  
Vol 9 (1) ◽  
pp. 34-40 ◽  
Author(s):  
P.J. Pradeep ◽  
T.C. Srijaya ◽  
A. Hussain ◽  
S. Mithun ◽  
F. Shaharom ◽  
...  

An attempt was made to study the trend in ammonia excretion by red hybrid tilapia during their acclimatization in seawater under slow and sudden conditions. Acclimatization of salinity 5 ppt showed an increase of ammonia level in water by 83.35% (7.69 mg/kg/l/day) as compared to the control group (P<0.05) whereas the value was 24.97% (10.25 mg/kg/l/day) at 10 ppt. The level of ammonia was dropped at salinity 15 ppt (5.12 mg/kg/l/day) by 50% and 25% at 20 ppt (3.84 mg/kg/l/day). The ammonia level with sudden transfer to different salinities were; 1.94, 1.38 and 2.77 mg/kg/l at 10, 20 and 30 ppt respectively, after 2 hrs of acclimatization whereas it reached to 11.11 mg/kg/l at 10 and 20 ppt within 24 hrs. All fishes were found dead within 3 hours of their sudden transfer to 30 ppt. The Results suggest that a gradual salinity increase is beneficial for acclimatizing marine tilapia.DOI: http://dx.doi.org/10.3126/on.v9i1.5730


Biotecnia ◽  
2012 ◽  
Vol 14 (2) ◽  
pp. 11 ◽  
Author(s):  
María Idalia Sandoval-Muy ◽  
Manuel Antonio Guereña-Araiza ◽  
Anselmo Miranda-Baeza ◽  
Martha Elisa Rivas-Vega

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
M. S. Ismail ◽  
A. Siti-Zahrah ◽  
M. R. M. Syafiq ◽  
M. N. A. Amal ◽  
M. Firdaus-Nawi ◽  
...  

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