scholarly journals Proteomic analysis reveals the differential proteins of endosperm between soft and hard wheat varieties

2019 ◽  
Vol 2 (2) ◽  
pp. 27-32 ◽  
Author(s):  
Feng Jia ◽  
Haicheng Yin ◽  
Qi Wang ◽  
Yu Chen ◽  
Jinshui Wang
Keyword(s):  
Proteomic Analysis ◽  
Wheat Varieties ◽  
Hard Wheat ◽  
Differential Proteins

scholarly journals Comparative Proteomic Analysis of Dipsacus asperoides Roots from Different Habitats in China

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3605
Author(s):  
Haijun Jin ◽  
Hua Yu ◽  
Haixia Wang ◽  
Jia Zhang
Keyword(s):  
Proteomic Analysis ◽  
Absolute Quantification ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Allene Oxide Cyclase ◽  
Protein Levels ◽  
Depth Analysis ◽  
Differential Proteins ◽  
Isobaric Tags

Dipsacus asperoides is a kind of Chinese herbal medicine with beneficial health properties. To date, the quality of D. asperoides from different habitats has shown significant differences. However, the molecular differences in D. asperoides from different habitats are still unknown. The aim of this study was to investigate the differences in protein levels of D. asperoides from different habitats. Isobaric tags for relative and absolute quantification (iTRAQ) and 2DLC/MS/MS were used to detect statistically significant changes in D. asperoides from different habitats. Through proteomic analysis, a total of 2149 proteins were identified, of which 42 important differentially expressed proteins were screened. Through in-depth analysis of differential proteins, the protein metabolism energy and carbohydrate metabolism of D. asperoides from Hubei Province were strong, but their antioxidant capacity was weak. We found that three proteins, UTP-glucose-1-phosphate uridylyltransferase, allene oxide cyclase, and isopentyl diphosphate isomerase 2, may be the key proteins involved in dipsacus saponin VI synthesis. Eight proteins were found in D. asperoides in response to environmental stress from different habitats. Quantitative real-time PCR analysis confirmed the accuracy and authenticity of the proteomic analysis. The results of this study may provide the basic information for exploring the cause of differences in secondary metabolites in different habitats of D. asperoides and the protein mechanism governing differences in quality.

scholarly journals Proteins in cord blood as a new target for prevention and treatment of bronchopulmonary dysplasia: a proteomic analysis

2021 ◽  
Author(s):  
Juan Wang ◽  
Jianping Mao ◽  
Gang Chen ◽  
Yuanmei Huang ◽  
Jinjin Zhou ◽  
...  
Keyword(s):  
Cord Blood ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Bronchopulmonary Dysplasia ◽  
Proteomic Analysis ◽  
Mapk Signaling ◽  
Term Infants ◽  
Reproductive Process ◽  
Prevention And Treatment ◽  
Differential Proteins

Abstract This study aimed to explore the proteins in cord blood that could regulate the development of neonatal bronchi and lungs, and to find a new target for the prevention and treatment of bronchopulmonary dysplasia (BPD). In this study, proteomic analysis was used to analyze the proteins in cord blood of preterm and term infants. A total of 100 differentially expressed (57 up-regulated and 43 down-regulated) proteins were identified from preterm with BPD and term infants cord blood (fold change ≥ 1.5, P value < 0.05). GO analysis revealed that the major enrichment functions of these differential proteins were multi-organism process, stimulus, immune system process growth, reproductive process, development process and antioxidant activity. The signaling pathways involved included insulin resistance, insulin signaling pathway, IL−17 signaling pathway, PI3K−Akt signaling pathway, NF−kappa B signaling pathway, glucagon signaling pathway, apoptosis, MAPK signaling pathway, as well as glycolysis/gluconeogenesis. Further protein and protein interact (PPI) analysis revealed that the interacting proteins involved were PGAM1, CNN2, HSP90AA and DAG1, which were all crucial in the development of BPD. We found that these differential proteins in cord blood might regulate bronchopulmonary development through the abovementioned signaling pathways or their interaction proteins, which may provide a new research direction for the intervention of BPD.

Influence of some digestive processes on the digestion by cattle of cereal grain fed whole

1978 ◽  
Vol 18 (90) ◽  
pp. 29 ◽  
Author(s):  
PC Toland
Keyword(s):  
Particle Size ◽  
Cereal Grains ◽  
Lower Percentage ◽  
Whole Grain ◽  
Wheat Grain ◽  
Wheat Varieties ◽  
Hard Wheat ◽  
Cereal Grain ◽  
Size Index

Pen studies were conducted with cattle in which the digestibility, rate of fermentation, site of digestion and grain loss in faeces were measured in diets comprising 4.8 kg of one or other of four cereal grains together with 2.4 kg of pasture hay. The cereals were Avon oats, Swan oats, Olympic wheat and Emblem wheat with natural weights of 48.3, 60.5, 81.3 and 78.8 kg hl-1 and particle size index 24, 35, 31 and 14, respectively. The apparent in vivo digestibility of Avon and Swan oats of 71.9 and 74.6 did not differ significantly (P > 0.05). However, there was significantly less grain cracked during initial mastication (12.5 vs. 19.2 per cent and a lower percentage of grain voided in faeces (4.3 vs. 9.3) for Avon compared with Swan oats (P < 0.05). The in vivo digestibility of wheat grain was significantly higher for Olympic than for Emblem (77.5 vs. 72.6 per cent) ;these performances were accompanied by lower percentages of grain cracked during initial mastication (17.0 vs. 22.3) and less whole grain DM voided in faeces (14.6 vs. 18.4 per cent) for Olympic compared with Emblem (P < 0.05). The major difference in the digestion of oat and wheat varieties was that rumination accounted for a high proportion of the breakdown of oat grain whereas the wheat varieties were digested by rumen fermentation with only a small proportion of grains broken down by rumination. The breakdown of whole grain in rumination was important for all rations. For the light oats, heavy oats, soft wheat and hard wheat, rumination accounted for 66 per cent, 44 per cent, 27 per cent and 17 per cent of the total breakdown respectively.

Varietal differences in rumen degradation of barley, wheat and hard wheat straws

1991 ◽  
Vol 53 (2) ◽  
pp. 143-150 ◽  
Author(s):  
M. Ramanzin ◽  
L. Bailoni ◽  
G. Beni
Keyword(s):  
Dry Matter ◽  
Minor Importance ◽  
Wheat Varieties ◽  
Varietal Differences ◽  
Hard Wheat ◽  
Rumen Degradation ◽  
Cereal Species ◽  
Dry Matter Degradation

AbstractThe rumen dry-matter degradation of straw from nine varieties of barley, nine varieties of wheat and seven varieties of hard wheat was studied to provide further information on varietal differences and their relationships with agronomic characteristics.In all the cereals there were significant differences in in situ dry-matter disappearance between varieties. The range of differences was lower in hard wheat varieties compared with the other cereals. There were no significant correlations between straw degradability and plant height, days from sowing to heading and grain production and quality. Lignin concentration was significantly correlated to straw degradability (r = −0·937 in barley, −0·675 in wheat and −0·987 in hard wheat).Leaf had lower concentrations of lignin and higher degradability than stem in barley and wheat but the two fractions were similar in hard wheat straws. The differences in degradation between the most and the least degradable straw varieties of each cereal species were mainly due to differences in degradability of both the fractions whereas the role of leaf and stem proportions was of minor importance.

scholarly journals Relationship between Yellow Alkaline Noodle Quality and Flour Properties in Hard Wheat Varieties in the Central Region of Japan

2008 ◽  
Vol 77 (4) ◽  
pp. 449-456 ◽  
Author(s):  
Masaya Fujita ◽  
Masako Seki ◽  
Hitoshi Matsunaka ◽  
Chikako Kiribuchi-Otobe ◽  
Ado Hiwatashi ◽  
...  
Keyword(s):  
Central Region ◽  
Wheat Varieties ◽  
Hard Wheat ◽  
Flour Properties ◽  
Yellow Alkaline Noodle ◽  
Noodle Quality

Comparative analysis of native and defatted flour from hard, extraordinarily soft, and medium‐hard wheat varieties for protein solvation, pasting, mixing, and dough rheological behavior

2019 ◽  
Vol 85 (1) ◽  
pp. 65-76
Author(s):  
Mehak Katyal ◽  
Narpinder Singh ◽  
Amardeep Singh Virdi ◽  
Amritpal Kaur ◽  
Arvind Kumar Ahlawat ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Rheological Behavior ◽  
Wheat Varieties ◽  
Hard Wheat

scholarly journals TMT-Based Quantitative Proteomic Analysis of Intestinal Organoids Infected by Listeria monocytogenes with Different Virulence

2020 ◽  
Author(s):  
Jie Huang ◽  
Cong Zhou ◽  
Guanghong Zhou ◽  
Keping Ye
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
High Performance ◽  
Virulent Strain ◽  
Cellular Protein ◽  
Quantitative Proteomic Analysis ◽  
Intestinal Organoids ◽  
Food Borne ◽  
Differential Proteins ◽  
Fold Change Cutoff

AbstractListeria monocytogenes (Lm) is an opportunistic food-borne pathogen that cause listeriosis. L. monocytogenes belonged to different serovars presents with different virulence in the host and caused different host reactions. To investigate the remodeling of host proteome by differently toxic strains, the cellular protein responses of intestinal organoids were analyzed using TMT labeling and high performance liquid chromatography-mass spectrometry. Quantitative proteomic analysis revealed 6564 differentially expressed proteins, of which 5591 proteins were quantified. The fold-change cutoff was set at 1.3 (Lm vs control), the virulent strain caused 102 up-regulated proteins and 52 down-regulated proteins, while the low virulent strain caused 188 up-regulated proteins and 25 down-regulated proteins. These identified proteins were involved in the regulation of essential processes such as biological metabolism, energy metabolism, and immune system process. Some selected proteins were screened by Real-time PCR and Western blotting. These results revealed that differently toxic L. monocytogenes induced similar biological functions and immune responses while had different regulation on differential proteins in the pathway.

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