Characterization of NPHS2 gene polymorphisms associated to steroid resistance nephrotic syndrome in Indian children

The present study aimed to evaluate the expression of CD80 and CD18 in subpopulations of peripheral blood leukocytes and oxidative kidney damage in rats with nephrotic syndrome (NS) induced by doxorubicin (Dox) in comparison to control animals at different time points. Male adult Wistar rats were submitted to 24-hour urine and blood collection for biochemical and immunological analysis at 7, 14, 21, and 28 days after Dox injection. After euthanasia, the kidneys were removed for histological analysis and the evaluation of oxidative stress. The phenotypic characterization of leukocytes was performed using flow cytometry. Dox-injected animals exhibited increased CD18 expression in cytotoxic T lymphocytes, NK cells, and monocytes and high CD80 expression in monocytes. Kidney oxidative damage was positively correlated with CD80 expression in monocytes and serum levels of creatinine. These results suggest that phagocytic and cytotoxic cells are preferentially recruited to the tissue injury site, which may contribute to kidney dysfunction in this animal model of NS. The blockade of integrin and costimulatory molecules may provide new therapeutic opportunities for NS.

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Markers of steroid resistance of the primary nephrotic syndrome in children

Practical medicine ◽

10.32000/2072-1757-2021-1-15-21 ◽

2021 ◽

Vol 19 (1) ◽

pp. 15-21

Author(s):

S. L. Morozov ◽

◽

V. V. Dlin ◽

Keyword(s):

Nephrotic Syndrome ◽

Steroid Therapy ◽

Molecular Genetic ◽

Clinical Importance ◽

Recent Decade ◽

Immunosuppressive Drugs ◽

Steroid Resistance ◽

Primary Nephrotic Syndrome ◽

Non Invasive ◽

The Common

The global task of the recent decade is to search for clinical and laboratory markers accurately showing a patient’s reaction to steroid therapy and other immunosuppressive drugs. It is important the applied methods and tests to be non-invasive and simple to use. The article considers various biomarkers used to verify the type of nephrotic syndrome depending on the sensitivity to steroid therapy. Besides the common markers, which are used in clinical practice or have shown a significant result, the work highlights the molecular- genetic markers of resistance to steroid therapy, which are of special clinical importance today. Also, the article presents authors’ own results in diagnosing the steroid resistance of the primary nephrotic syndrome.

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NPHS2 gene mutation, atopy, and gender as risk factors for steroid-resistant nephrotic syndrome in Indonesians

Paediatrica Indonesiana ◽

10.14238/pi51.5.2011.272-6 ◽

2011 ◽

Vol 51 (5) ◽

pp. 272 ◽

Cited By ~ 1

Author(s):

Dedi Rachmadi ◽

Danny Hilmanto ◽

Ponpon Ijradinata ◽

Abdurahman Sukadi

Keyword(s):

Risk Factors ◽

Nephrotic Syndrome ◽

Gene Mutation ◽

Significant Risk ◽

Gene Mutations ◽

Male Gender ◽

Amplification Refractory Mutation System ◽

Steroid Resistant ◽

Steroid Resistant Nephrotic Syndrome ◽

Nphs2 Gene

Background Steroid-resistant nephrotic syndrome (SRNS) often develops into end stage renal disease. Previous studies have reported that NPHS2 gene mutation, gender, and atopic history are risk factors associated with SRNS. Interethnic, sociocultural, and environmental differences have also been suggested to affect these mutations.Objective To analyze possible risk factors for SRNS, including NPHS2 gene mutations (412C→T and 419delG), gender and atopic history, in Indonesian subjects with SRNS.Methods A case-control study with 153 subjects, consisting of 88 SRNS patients and 65 control subjects, was undertaken in 10 Indonesian teaching centre hospitals from September 2006 to December 2007. Analysis of the NPHS2 gene mutation in 412 C→T was performed by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), while that for the NPHS2 gene mutation in 419delG was performed by restriction fragment length polymorphism (RFLP). Data was analyzed by multiple logistic regression.Results In our Indonesian subjects, the significant risk factors for SRNS were male gender (OR=2.21; CI 95%:1.07-4.56, P=0.036), NPHS2 412C→T gene mutation (OR=18.07; CI 95%:6.76-48.31, P<0.001), and NPHS2 419delG gene mutation (OR=4.55; CI 95%:1.66-12.47, P=0.003). However, atopic history was not a significant risk factor for SRNS (OR=1.807; CI 95%:0.642-5.086, P=0.262).Conclusion NPHS2 412C→T and 419delG gene mutations, as well as male gender are risk factors for SRNS in Indonesian subjects. Atopic history was not significantly associated with SRNS in our subjects. [Paediatr Indones. 2011;51:272-6].

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Characterization of arginase 1 gene polymorphisms in the Algerian population and association with blood pressure

Clinical Biochemistry ◽

10.1016/j.clinbiochem.2009.03.004 ◽

2009 ◽

Vol 42 (10-11) ◽

pp. 1178-1182 ◽

Cited By ~ 16

Author(s):

Djabaria Meroufel ◽

Julie Dumont ◽

Sounnia Médiène-Benchekor ◽

Soraya Benhammamouch ◽

Pierre Ducimetière ◽

...

Keyword(s):

Blood Pressure ◽

Gene Polymorphisms ◽

Arginase 1 ◽

Algerian Population

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ASSOTIATIONS OF PECULIARITIES OF HLA-PHENOTYPE AND THE SENSIBILITY TO THE CORTICOSTEROID TREATMENT IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS WITH NEPHROTIC SYNDROME

Ukrainian Journal of Nephrology and Dialysis ◽

10.31450/ukrjnd.1(37).2013.07 ◽

2017 ◽

pp. 37-44

Author(s):

M. Kolesnyk ◽

G. Drannik ◽

V. Driyanska ◽

O. Petrina ◽

M. Velychko

Keyword(s):

Nephrotic Syndrome ◽

Hla Antigens ◽

Corticosteroid Treatment ◽

Steroid Resistance ◽

Control Group ◽

Chronic Glomerulonephritis ◽

Healthy Donors ◽

Steroid Sensitive ◽

Steroid Sensitivity ◽

Hla Phenotype

The purpose of study was determination of HLA -antigens I and II classes as predictors of ineffectiveness of initial steroid therapy, and according prognozonegative markers of chronic glomerulonephritis with nephrotic syndrome. Methods. In 59 chronic glomerulonephritis with nephrotic syndrome patients (steroid sensitive n=33 (1 gr.) and steroid resistant’s n= 26 (2 gr.)) and 350 healthy donors( control group) studied HLA antigens I and II classes of the special anti- HLA-antigens panel (20 antigens of locus A, 31 – of locus B and 9- of locus DR). Result. In patients with chronic glomerulonephritis, nephrotic syndrome with hormone sensitivity relative risk is high at the presents of A28 (RR=8,5, r р <0,001), it made attributive risk (=0,37). In comparison with a control group, RR>2 for antigens A11 (RR=2,23), A23 (RR=4,28), A24 (RR=3,3), A29 (RR=10,78) that A30 (RR=11,23); attributive risk more than 0,1 for the antigen A11 (=0,16) ; A24 (=0,13), other did not differ from control. Subzero connection is exposed for the antigens of A2 (р<0,001), А9 (р=0,007). In locus antigen B14 (RR=5,65, р =0,001) are exposed, B44 (RR=48,25, р =0,004), B51(RR=12,32, р =0,006) and attributive risk of development of disease (according =0,24, 0,12 ; 0,14); and antigens B38 and B41 (RR=11,57, р=0,05). The steroid sensitivity was associated with the antigens B5 (p=0,033), B12 (p=0,005) and B35 (p=0,021). In locus DR made etiologic faction antigens DR4 (RR=7,0 and =0,24) DRw52 (RR=7,0 and =0,25). Conclusions. For patients with chronic glomerulonephritis with a nephrotic syndrome antigens of HLA-B14,B38, B51, DRw52 are associated with steroid sensitivity. The attributive risk of steroid resistance is high for split A19+31+32, antigens B8, B55.

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