Plausible challenges of methicillin and clindamycin resistance detection in Staphylococcus aureus

Gene Reports ◽  
2021 ◽  
Vol 24 ◽  
pp. 101179
Author(s):  
Nasim Asadi Faezi ◽  
Alka Hasani ◽  
Elghar Soltani ◽  
Vahideh Valizadeh ◽  
Akbar Hasani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Detection

scholarly journals Screening and detection of heterogenous vancomycin intermediate Staphylococcus aureus in Hospital Kuala Lumpur Malaysia, using the glycopeptide resistance detection etest and population analysis profiling

2012 ◽  
Vol 4 (1) ◽  
pp. 20 ◽  
Author(s):  
Siti Roszilawati Ramli ◽  
Hui-min Neoh ◽  
Muhammad Nazri Aziz ◽  
Salasawati Hussin
Keyword(s):  
Staphylococcus Aureus ◽  
Prevalence Rate ◽  
Population Analysis ◽  
Kuala Lumpur ◽  
Glycopeptide Resistance ◽  
Methicillin Resistant ◽  
First Report ◽  
Resistance Detection

In a 3-month study done in Hospital Kuala Lumpur (HKL), 7 out of 320 methicillin resistant <em>Staphylococcus aureus </em>isolates were confirmed as heterogeneous vancomycin intermediate S. aureus (hVISA) using the glycopeptide resistance detection e-test and population analysis, giving a prevalence rate of 2.19%. This is the first report of hVISA in Malaysia.

scholarly journals Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: A Narrative Review

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 208
Author(s):  
Andrea Sanchini
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Food Poisoning ◽  
Opportunistic Pathogen ◽  
Narrative Review ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
Wide Range ◽  
Resistance Detection

Staphylococcus aureus is an opportunistic pathogen responsible for a wide range of infections in humans, such as skin and soft tissue infections, pneumonia, food poisoning or sepsis. Historically, S. aureus was able to rapidly adapt to anti-staphylococcal antibiotics and become resistant to several classes of antibiotics. Today, methicillin-resistant S. aureus (MRSA) is a multidrug-resistant pathogen and is one of the most common bacteria responsible for hospital-acquired infections and outbreaks, in community settings as well. The rapid and accurate diagnosis of antimicrobial resistance in S. aureus is crucial to the early initiation of directed antibiotic therapy and to improve clinical outcomes for patients. In this narrative review, I provide an overview of recent phenotypic and molecular diagnostic methods for antimicrobial resistance detection in S. aureus, with a particular focus on MRSA detection. I consider methods for resistance detection in both clinical samples and isolated S. aureus cultures, along with a brief discussion of the advantages and the challenges of implementing such methods in routine diagnostics.

scholarly journals Screening and Detection of Heterogenous Vancomycin Intermediate Staphylococcus aureus in Hospital Kuala Lumpur Malaysia, Using the Glycopeptide Resistance Detection Etest and Population Analysis Profiling

2012 ◽  
Vol 4 (1) ◽  
pp. 71-72
Author(s):  
Siti Roszilawati Ramli ◽  
Hui-min Neoh ◽  
Muhammad Nazri Aziz ◽  
Salasawati Hussin
Keyword(s):  
Staphylococcus Aureus ◽  
Prevalence Rate ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Population Analysis ◽  
Kuala Lumpur ◽  
Glycopeptide Resistance ◽  
Methicillin Resistant ◽  
First Report ◽  
Resistance Detection

In a 3-month study done in Hospital Kuala Lumpur (HKL), 7 out of 320 methicillin resistant Staphylococcus aureus isolates were confirmed as heterogeneous vancomycin intermediate S. aureus (hVISA) using the glycopeptide resistance detection e-test and population analysis, giving a prevalence rate of 2.19%. This is the first report of hVISA in Malaysia.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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