Antitumor activity of Ankaferd Blood Stopper® on MCF-7 breast cancer: A proteomic approach to ascertain the mechanism of the action

2021 ◽  
Vol 28 ◽  
pp. 100449
Author(s):  
Özge Cansın Zeki ◽  
Merve Nenni ◽  
Mustafa Çelebier ◽  
Selin Öncül ◽  
Ayşe Ercan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Proteomic Approach ◽  
Ankaferd Blood Stopper ◽  
Mcf 7

scholarly journals Antitumor activity of the Ailanthus altissima bark phytochemical ailanthone against breast cancer MCF‑7 cells

2018 ◽  
Author(s):  
Ruxing Wang ◽  
Yanjie Lu ◽  
Hong Li ◽  
Lixin Sun ◽  
Ning Yang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Ailanthus Altissima ◽  
Mcf 7

scholarly journals Solid Polymeric Nanoparticles of Albendazole: Synthesis, Physico-Chemical Characterization and Biological Activity

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5130 ◽  
Author(s):  
Roxana Racoviceanu ◽  
Cristina Trandafirescu ◽  
Mirela Voicu ◽  
Roxana Ghiulai ◽  
Florin Borcan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Benzimidazole Derivative ◽  
Chemical Characterization ◽  
Breast Cancer Cell Lines ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Encapsulation Process ◽  
Mcf 7

Albendazole is a benzimidazole derivative with documented antitumor activity and low toxicity to healthy cells. The major disadvantage in terms of clinical use is its low aqueous solubility which limits its bioavailability. Albendazole was incorporated into stable and homogeneous polyurethane structures with the aim of obtaining an improved drug delivery system model. Spectral and thermal analysis was used to investigate the encapsulation process and confirmed the presence of albendazole inside the nanoparticles. The in vitro anticancer properties of albendazole encapsulated in polyurethane structures versus the un-encapsulated compound were tested on two breast cancer cell lines, MCF-7 and MDA-MB-231, in terms of cellular viability and apoptosis induction. The study showed that the encapsulation process enhanced the antitumor activity of albendazole on the MCF-7 and MDA-MB-23 breast cancer lines. The cytotoxic activity manifested in a concentration-dependent manner and was accompanied by changes in cell morphology and nuclear fragmentation.

scholarly journals Effects of stealth liposomal daunorubicin plus tamoxifen on the breast cancer and cancer stem cells

2010 ◽  
Vol 13 (2) ◽  
pp. 136 ◽  
Author(s):  
Jia Guo ◽  
Wan-Liang Lu
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Synergistic Effects ◽  
Inhibitory Effects ◽  
Liposomal Daunorubicin ◽  
Mcf 7

PURPOSE: The cancer stem cells play an important role in the invasion, metastasis and relapse of cancers as they are resistant to regular chemotherapy. In the present study, stealth liposomal daunorubicin plus tamoxifen was developed for eradicating breast cancer cells together with cancer stem cells. METHODS: Inhibitory effects were performed on the bulk human breast cancer cells (MCF-7), the sorted MCF-7 cancer stem-like cells (side population, SP), and the sorted MCF-7 cancer cells (NSP), respectively. Antitumor activity and TUNEL analysis were evaluated on the MCF-7 xenografts in nude mice. RESULTS: The encapsulation efficiencies of daunorubicin and tamoxifen were 95% and 90%, respectively. The mean particle size of the stealth liposomes was about 100 nm. Breast cancer stem cells were identified by the specific markers CD44+/CD24-, and isolated from bulk MCF-7 cells. When applying stealth liposomal daunorubicin plus tamoxifen, the inhibitory effects on both the breast cancer cells and the cancer stem cells were significantly increased in vitro, respectively. In the MCF-7 xenografts in mice, stealth liposomal daunorubicin plus tamoxifen showed the most favorable antitumor activity due to the passive targeting the tumor tissue and the synergistic effects in eliminating breast cancer cells and cancer stem cells. CONCLUSION: Stealth liposomal daunorubicin plus tamoxifen could have the potentials in eliminating both breast cancer cells and cancer stem cells.

Synthesis and Antitumor Activity of Some N2-(Thien-3-yl)amidrazones

2014 ◽  
Vol 69 (7) ◽  
pp. 811-816 ◽  
Author(s):  
Mohammed M. Abadleh ◽  
Mustafa M. El-Abadelah ◽  
Salim S. Sabri ◽  
Hanan H. Mohammed ◽  
Malek A. Zihlif ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Cell Viability ◽  
Cell Lines ◽  
K562 Cell ◽  
Cyclic Amine ◽  
Cell Viability Assay ◽  
Viability Assay ◽  
A Cell ◽  
Mcf 7

6aA set of new N2-(thien-3-yl)amidrazones (-h) incorporating N-piperazines and related congeners has been synthesized by reacting the hydrazonoyl chloride 4(derived from 3-aminothiophene- 2-carboxylate) with the appropriate sec-cyclic amine. The antitumor activity of these compounds was evaluated on breast cancer (MCF-7) and leukemic (K562) cell lines by a cell viability assay utilizing the tetrazolium dye (MTT). The amidrazone 6d encompassing the N-piperazine moiety, was the most active against MCF-7 and K562 with IC50 of 7.28 and 9:91 μM, respectively.

scholarly journals Artemisinic acid exhibits antitumor activity in MCF-7 breast cancer cells through the inhibition of angiogenesis, VEGF, m-TOR and AKT signalling pathways

2016 ◽  
Vol 11 (3) ◽  
pp. 691
Author(s):  
Yan Cui ◽  
Hui Ren ◽  
Hao-Chang Li ◽  
Quan-Shi Wang
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Tube Formation ◽  
Signalling Pathways ◽  
Artemisinic Acid ◽  
Mcf 7

<p>The aim of the present study was to evaluate the antitumor and anti-angiogenic effects of artemisinic acid in MCF-7 human breast cancer cells. Various cell signalling pathways (VEGF, m-TOR and AKT signalling pathways) and MTT assay were used. The in vivo antitumor activity of artemisinic acid was evaluated by means of tumor xenograft mouse model. Transwell cell migration assay was used to examine the chemotactic motility of the human umbilical vascular endothelial cells (HUVECs), while as endothelial cell capillary-like tube formation assay was used to evaluate the effect of artemisinic acid on the tube formation in HUVECs. We found that artemisinic acid considerably reduced both the volume and weight of concrete tumors and reduced angiogenesis in a xenograft mouse tumor model in vivo. Further, artemisinic acid suppressed the VEGF-induced cell migration and capillary-like tube formation of HUVECs in a dose-dependent manner. Artemisinic acid was found to suppress the VEGF-induced phosphorylation of VEGFR2 and also the activity of AKT and m-TOR.</p><p><strong>Video Clip:</strong></p><p><a href="https://youtube.com/v/w7jZqcDvMTc">Western blot assay:</a> 4 min 33 sec </p>

Identification of Proteins Secreted from Leptin Stimulated MCF-7 Breast Cancer Cells: A Dual Proteomic Approach

2008 ◽  
Vol 233 (6) ◽  
pp. 708-720 ◽  
Author(s):  
Candida N. Perera ◽  
Heather S. Spalding ◽  
Sulma I. Mohammed ◽  
Ignacio G. Camarillo
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Proteomic Approach ◽  
Mcf 7
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