scholarly journals B-PO05-171 ASSOCIATION OF LEFT VENTRICULAR TISSUE HETEROGENEITY ON COMPUTED TOMOGRAPHY WITH VENTRICULAR ARRHYTHMIAS IN ISCHEMIC CARDIOMYOPATHY PATIENTS

Heart Rhythm ◽  
2021 ◽  
Vol 18 (8) ◽  
pp. S441-S442
Author(s):  
Usama Daimee ◽  
Eric Sung ◽  
Marc Engels ◽  
Ronald D. Berger ◽  
Natalia A. Trayanova ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Ventricular Arrhythmias ◽  
Ischemic Cardiomyopathy ◽  
Left Ventricular ◽  
Tissue Heterogeneity ◽  
Left Ventricular Tissue ◽  
Ventricular Tissue

Abstract 19706: CD39/CD73 Pathway in End-stage Human Ischemic Cardiomyopathy

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Tatiana Novitskaya ◽  
Yan Ru Su ◽  
Simon Maltais ◽  
Tarek S Absi ◽  
Richard J Gumina
Keyword(s):  
Protein Level ◽  
Ischemic Cardiomyopathy ◽  
Ischemia Reperfusion ◽  
Immunohistochemical Analysis ◽  
Left Ventricular ◽  
Mrna Levels ◽  
Protein Levels ◽  
Left Ventricular Tissue ◽  
Ventricular Tissue ◽  
End Stage

Introduction: CD39 (ectonucleoside triphosphate diphosphohydrolase) is a nucleotidase expressed on endothelial cells, vascular smooth muscles cells, and leukocytes. CD39 plays a key role in vascular homeostasis, hydrolyzing extracellular ATP or ADP to AMP. CD73 (ecto-5’-nculeotidase) hydrolyzes AMP to adenosine. Both CD39 and CD73 are key modulators of purinergic signaling and have been shown to be important in ischemic preconditioning and ischemia reperfusion in animal models. Hypothesis: We hypothesized that the expression of the purine nucleotide-metabolizing pathway consisting of CD39 and CD73 in human hearts with ischemic cardiomyopathy is unregulated in response to ischemia. Methods: We compared the expression of CD39 and CD73 in left ventricular samples from patients with end-stage ischemic cardiomyopathy and normal hearts. Left ventricular tissue obtained from 7 end-stage ischemic cardiomyopathy hearts and 7 normal hearts not used for transplantation were homogenized and the levels of CD39 and CD73 mRNA were measured by qRT-PCR. Using the same tissue, immunoblot analysis for the protein level of CD39 and CD73 was conducted. Furthermore, immunohistochemical analysis of CD39 and CD73 expression was conducted on left ventricular tissue from the same subjects. Results: The total mRNA levels of CD39 and CD73 were increased in patients with end-stage ischemic cardiomyopathy. However, while there was a reduction in the total CD73 protein levels that did not reach significance, there was a marked reduction in the the total protein level of CD39 in ICM hearts (Figure). Immunohistochemical analysis demonstrated that the expression pattern of CD39 was significantly different in ICM hearts compared to normal hearts. Conclusions: These data provide important evidence that the extracellular nucleotide metabolizing pathway is altered in ICM. CD39 protein levels are significantly reduced in human end-stage ischemic cardiomyopathy.

Immersion before dry simulated dive reduces cardiomyocyte function and increases mortality after decompression

2010 ◽  
Vol 109 (3) ◽  
pp. 752-757 ◽  
Author(s):  
Svein Erik Gaustad ◽  
Alf O. Brubakk ◽  
Morten Høydal ◽  
Daniele Catalucci ◽  
Gianluigi Condorelli ◽  
...  
Keyword(s):  
Cardiac Function ◽  
Survival Time ◽  
Left Ventricular ◽  
Endoplasmic Reticulum Calcium ◽  
Protein Levels ◽  
Left Ventricular Tissue ◽  
Bubble Detection ◽  
Ventricular Tissue ◽  
The Right ◽  
Phospholamban Phosphorylation

Diving and decompression performed under immersed conditions have been shown to reduce cardiac function. The mechanisms for these changes are not known. The effect of immersion before a simulated hyperbaric dive on cardiomyocyte function was studied. Twenty-three rats were assigned to four groups: control, 1 h thermoneutral immersion, dry dive, and 1 h thermoneutral immersion before a dive (preimmersion dive). Rats exposed to a dive were compressed to 700 kPa, maintained for 45 min breathing air, and decompressed linearly to the surface at a rate of 50 kPa/min. Postdive, the animals were anesthetized and the right ventricle insonated for bubble detection using ultrasound. Isolation of cardiomyocytes from the left ventricle was performed and studied using an inverted fluorescence microscope with video-based sarcomere spacing. Compared with a dry dive, preimmersion dive significantly increased bubble production and decreased the survival time (bubble grade 1 vs. 5, and survival time 60 vs. 17 min, respectively). Preimmersion dive lead to 18% decreased cardiomyocyte shortening, 20% slower diastolic relengthening, and 22% higher calcium amplitudes compared with controls. The protein levels of the sarco-endoplasmic reticulum calcium ATPase (SERCA2a), Na+/Ca2+ exchanger (NCX), and phospholamban phosphorylation in the left ventricular tissue were significantly reduced after both dry and preimmersion dive compared with control and immersed animals. The data suggest that immersion before a dive results in impaired cardiomyocyte and Ca2+ handling and may be a cellular explanation to reduced cardiac function observed in humans after a dive.

Effect of Exercise Preconditioning on Doxorubicin Accumulation in Left Ventricular Tissue

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 163
Author(s):  
Brock T. Jensen ◽  
David S. Hydock ◽  
Chia-Ying Lien ◽  
Carole M. Schneider ◽  
Reid Hayward
Keyword(s):  
Left Ventricular ◽  
Left Ventricular Tissue ◽  
Ventricular Tissue ◽  
Exercise Preconditioning

scholarly journals Impact of Arterial Load and Loading Sequence on Left Ventricular Tissue Velocities in Humans

2007 ◽  
Vol 50 (16) ◽  
pp. 1570-1577 ◽  
Author(s):  
Barry A. Borlaug ◽  
Vojtech Melenovsky ◽  
Margaret M. Redfield ◽  
Kristy Kessler ◽  
Hyuk-Jae Chang ◽  
...  
Keyword(s):  
Left Ventricular ◽  
Left Ventricular Tissue ◽  
Ventricular Tissue ◽  
Arterial Load ◽  
Loading Sequence

scholarly journals The Effects of Aqueous Extract from Nardostachys chinensis Batalin on Blood Pressure and Cardiac Hypertrophy in Two-Kidney One-Clip Hypertensive Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Rayile Aisa ◽  
Zhaoxia Yu ◽  
Xiangyang Zhang ◽  
Dilinuer Maimaitiyiming ◽  
Lipeng Huang ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiac Hypertrophy ◽  
Aqueous Extract ◽  
Caspase 3 ◽  
Left Ventricular ◽  
Left Renal Artery ◽  
Hypertensive Rats ◽  
Left Ventricular Tissue ◽  
Nardostachys Chinensis ◽  
Ventricular Tissue

Aims. The aim of this study was to investigate the effects of the aqueous extract of Nardostachys chinensis Batalin (NCBAE) on blood pressure and cardiac hypertrophy using two-kidney one-clip (2K1C) hypertensive rats. Methods. 2K1C rat models were set up by clipping the left renal artery. Sham-operated rats underwent the same surgical procedure except for renal arterial clipping. 2K1C hypertensive rats were orally given NCBAE at doses of 210, 420, and 630 mg·kg−1·d−1 for 6 weeks. Twelve weeks after surgery, rat SBP and echocardiographic parameters were measured, cardiac histopathology was assessed, serum NO and LDH were detected, and the expression of Bcl-2 and caspase-3 of left ventricular tissue was assessed by western blot. Results. Treatment with NCBAE resulted in a decrease of SBP, LVPWd, LVPWs, IVSd, IVSs, LVW/BW ratio, and cardiomyocyte CSA, an increase of LVEF, and inhibition of 2K1C-induced reduction in serum NO and elevation of LDH compared with 2K1C group. NCBAE intervention also showed a significant increase of Bcl-2 expression and reduction of cleaved caspase-3 level dose-dependently in left ventricular tissue. Conclusion. Our data demonstrate that NCBAE has an antihypertensive property and protective effect on 2K1C-induced cardiac hypertrophy especially at the dose of 630 mg·kg−1·d−1.

scholarly journals First-in-man experience with ultrasound renal denervation for treatment of recurrent ventricular arrhythmias

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
D.N Millenaar ◽  
F Mahfoud ◽  
V Pavlicek ◽  
L Lauder ◽  
M Boehm ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Catheter Ablation ◽  
Ejection Fraction ◽  
Ventricular Arrhythmias ◽  
Ischemic Cardiomyopathy ◽  
Renal Denervation ◽  
Left Ventricular ◽  
Funding Source ◽  
Ultrasound Energy

Abstract Background/Introduction Ventricular arrhythmias (VA) are common in patients with chronic heart failure (CHF) and can be refractory to drugs and catheter ablation. Promising results of sympathomodulatory treatment have been reported in these patients. Purpose This first in man study aims at investigating catheter-based renal denervation (RDN) using ultrasound energy for treatment of refractory VA in patients with CHF. Methods Four patients (age 65±10 years, all male, left ventricular ejection fraction 36±7%, global longitudinal strain (GLS) −10±3%) with CHF (n=1 ischemic cardiomyopathy, n=3 non-ischemic cardiomyopathy) and refractory VA were treated with RDN using ultrasound energy. All patients had undergone endo- or epicardial catheter ablation for recurrent ventricular tachycardia (VT) or fibrillation (VF) in the past and were on at least 2 antiarrhythmic drugs. Computer tomography angiography was performed at baseline, duplex ultrasound of renal arteries, ambulatory blood pressure monitoring (ABPM) and ICD interrogations were performed before, 1 day and 3 months post RDN. Results Bilateral RDN using an ultrasound-based catheter were performed with at least 2 sonications in each main branch of the left and right renal artery. In this analysis, mean follow-up time was 113±12 days. All RDN procedures were performed without any complications. No renal artery stenoses during follow-up. Arrhythmic burden (measured as VT/VF episodes) within 3 months before RDN requiring ICD therapy was reduced from 3 [1.5–54.5] episodes of anti-tachycardia pacing (ATP) and 0.5 [0–1.25] adequate ICD shocks to 1 [0.75–1] episode of ATP. There were no adequate ICD shocks after 3 months. Mean 24-hour ABP before RDN was 94±8/65±9 mmHg with no change in BP following 3 months (SBP 92±1 mmHg, DBP 62±6 mmHg after 3 months). There was no change in left ventricular GLS (−10±3% before, −9±4% after RDN) or ejection fraction (36±7% before and after RDN). Conclusions RDN using ultrasound energy in patients with CHF and refractory VA was safely performed with no changes in blood pressure and reduced the arrhythmic burden after 3 months follow-up. Funding Acknowledgement Type of funding source: Private grant(s) and/or Sponsorship. Main funding source(s): ReCor Medical Inc.

scholarly journals Balloon Type Elasticity Sensing of Left Ventricular Tissue for Small Experimental Animals

2011 ◽  
Vol 47 (12) ◽  
pp. 648-655 ◽  
Author(s):  
Mitsuru HIGASHIMORI ◽  
Ryohei ISHII ◽  
Kenjiro TADAKUMA ◽  
Makoto KANEKO ◽  
Syunsuke TAMAKI ◽  
...  
Keyword(s):  
Left Ventricular ◽  
Experimental Animals ◽  
Left Ventricular Tissue ◽  
Ventricular Tissue
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