Prevalence and characterization of fibrosis in surveillance liver biopsies of patients with Fontan circulation

2016 ◽  
Vol 57 ◽  
pp. 106-115 ◽  
Author(s):  
Lea F. Surrey ◽  
Pierre Russo ◽  
Jack Rychik ◽  
David J. Goldberg ◽  
Kathryn Dodds ◽  
...  
Keyword(s):  
Fontan Circulation ◽  
Liver Biopsies

Hepatic fibrosis and cirrhosis in the Fontan circulation: a detailed morphological study

2007 ◽  
Vol 61 (4) ◽  
pp. 504-508 ◽  
Author(s):  
T J Kendall ◽  
B Stedman ◽  
N Hacking ◽  
M Haw ◽  
J J Vettukattill ◽  
...  
Keyword(s):  
Preoperative Assessment ◽  
Fontan Circulation ◽  
Severe Tricuspid Regurgitation ◽  
Histological Features ◽  
Sinusoidal Dilatation ◽  
Hepatocellular Damage ◽  
Biopsy Specimens ◽  
Inflammatory Mechanism ◽  
Spur Formation ◽  
Liver Biopsies

Aims:To describe the histological features of the liver in patients with a Fontan circulation.Methods:Specimens from liver biopsies carried out as part of preoperative assessment prior to extracardiac cavopulmonary conversion of an older style Fontan were examined and scored semi-quantitatively for pertinent histological features. To support the use of the scoring, biopsy specimens were also ranked by eye for severity to allow correlation with assigned scores.Results:Liver biopsy specimens from 18 patients with a Fontan circulation were assessed. All specimens showed sinusoidal fibrosis. In 17 cases there was at least fibrous spur formation, with 14 showing bridging fibrosis and 2 showing frank cirrhosis. In 17 cases at least some of the dense or sinusoidal fibrosis was orcein positive, although a larger proportion of the dense fibrous bands were orcein positive compared with the sinusoidal component. All specimens showed marked sinusoidal dilatation, and 14 showed bile ductular proliferation; 1 showed minimal iron deposition, and 1 showed mild lobular lymphocytic inflammation. There was no cholestasis or evidence of hepatocellular damage. Similar appearances were observed in 2 patients with severe tricuspid regurgitation.Discussion:The histological features of the liver in patients with a Fontan circulation are similar to those described in cardiac sclerosis. Sinusoidal dilatation and sinusoidal fibrosis are marked in the Fontan series. The presence of a significant amount of orcein negative sinusoidal fibrosis suggests there may be a remediable component, although the dense fibrous bands are predominantly orcein positive, suggesting chronicity and permanence. No inflammation or hepatocellular damage is evident, suggesting that fibrosis may be mediated by a non-inflammatory mechanism.

scholarly journals Characterization of the liver immune microenvironment in liver biopsies from patients with chronic HBV infection

JHEP Reports ◽  
2021 ◽  
pp. 100388
Author(s):  
Nicholas van Buuren ◽  
Ricardo Ramirez ◽  
Scott Turner ◽  
Diana Chen ◽  
Vithika Suri ◽  
...  
Keyword(s):  
Hbv Infection ◽  
Immune Microenvironment ◽  
Chronic Hbv Infection ◽  
Liver Biopsies ◽  
Chronic Hbv

Isolation, Culture and Characterization of Adult Human Hepatocytes from Surgical Liver Biopsies

Hepatology ◽  
1984 ◽  
Vol 4 (5) ◽  
pp. 849-854 ◽  
Author(s):  
Francois Ballet ◽  
Marie-Elisabeth Bouma ◽  
Shu-Ren Wang ◽  
Norma Amit ◽  
Jacqueline Marais ◽  
...  
Keyword(s):  
Human Hepatocytes ◽  
Adult Human ◽  
Liver Biopsies

Characterization of Circulating and Urinary Biomarkers in the Fontan Circulation and Their Correlation With Cardiac Imaging

2022 ◽  
Vol 162 ◽  
pp. 177-183
Author(s):  
Sunil J. Ghelani ◽  
Alexander R. Opotowsky ◽  
David M. Harrild ◽  
Andrew J. Powell ◽  
Nina Azcue ◽  
...  
Keyword(s):  
Cardiac Imaging ◽  
Fontan Circulation ◽  
Urinary Biomarkers

Contrast-Enhanced Ultrasound for Guidance of Percutaneous Liver Interventions in Neoplastic Hepatic Disease

2017 ◽  
Vol 01 (03) ◽  
pp. 201-207
Author(s):  
Sasan Partovi ◽  
Dean Nakamoto ◽  
Ziang Lu ◽  
Alice Yu ◽  
Indravadan Patel ◽  
...  
Keyword(s):  
Image Guidance ◽  
Ultrasound Guidance ◽  
Target Lesion ◽  
Hepatic Disease ◽  
Focal Liver Lesions ◽  
Contrast Enhanced Ultrasound ◽  
Resonance Imaging ◽  
Contrast Enhanced ◽  
Liver Biopsies

AbstractContrast-enhanced ultrasound (CEUS) is an evolving technique for the detection and characterization of focal liver lesions. Currently, it is recommended mainly for inconclusive cases and for patients with contraindications to computed tomography (CT)/magnetic resonance imaging. For percutaneous liver interventions, image guidance is of utmost importance to detect the target lesion. Primarily, ultrasound guidance is used for liver biopsies and ablations. CT is reserved for challenging cases, for example, when the target or the surrounding structures are not well identified with standard B-mode ultrasound. Instead of CT guidance, CEUS has gained increasing interest for percutaneous liver interventions. CEUS has the advantage of superior target visualization, real-time imaging guidance of probe or needle advancement without exposure to radiation.

Crystalloid Inclusions in Hepatocyte Mitochondria and Their Similarity to Cytoplasmic Crystalloids

1974 ◽  
Vol 32 ◽  
pp. 198-199
Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Special Interest ◽  
Structural Variations ◽  
Mitochondrial Alterations ◽  
The Matrix ◽  
Crystalloid Inclusions ◽  
Liver Biopsies

Mitochondrial alterations were studied in 25 liver biopsies from patients with alcoholic liver disease. Of special interest were the morphologic resemblance of certain fine structural variations in mitochondria and crystalloid inclusions. Four types of alterations within mitochondria were found that seemed to relate to cytoplasmic crystalloids.Type 1 alteration consisted of localized groups of cristae, usually oriented in the long direction of the organelle (Fig. 1A). In this plane they appeared serrated at the periphery with blind endings in the matrix. Other sections revealed a system of equally-spaced diagonal lines lengthwise in the mitochondrion with cristae protruding from both ends (Fig. 1B). Profiles of this inclusion were not unlike tangential cuts of a crystalloid structure frequently seen in enlarged mitochondria described below.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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