Comparison of sulfur hexafluoride tracer technique, rumen simulation technique and in vitro gas production techniques for methane production from ruminant feeds

Recently, the automatic in vitro gas production techniques (e.g. Cone. 1994; Theodorou et al., 1994) have been developed to study rumen fermentation kinetics. Many approaches have been taken. This work investigates the suitability of different methods for estimating the rumen fermentation of two starch rich feedstuffs.

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Effect of Ginger Essential Oil on in Vitro Gas Production, Rumen Fermentation and Methane Production

Tarım Bilimleri Dergisi ◽

10.15832/ankutbd.734827 ◽

2021 ◽

pp. 509-515

Author(s):

Erdinç ALTINÇEKİÇ ◽

Önder CANBOLAT ◽

Şeniz ÖZİŞ ALTINÇEKİÇ

Keyword(s):

Essential Oil ◽

Methane Production ◽

Rumen Fermentation ◽

Gas Production ◽

In Vitro Gas Production

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Silage of Intercropping Corn, Palisade Grass, and Pigeon Pea Increases Protein Content and Reduces In Vitro Methane Production

Agronomy ◽

10.3390/agronomy10111784 ◽

2020 ◽

Vol 10 (11) ◽

pp. 1784

Author(s):

Beatriz Ligoski ◽

Lucas Ferreira Gonçalves ◽

Flavio Lopes Claudio ◽

Estenio Moreira Alves ◽

Ana Maria Krüger ◽

...

Keyword(s):

Chemical Composition ◽

Methane Production ◽

Nutritional Quality ◽

Production Systems ◽

Animal Feed ◽

Lignin Content ◽

Pigeon Pea ◽

Gas Production ◽

In Vitro Gas Production

Legume–grass intercropping systems are a sustainable option to improve nutritional quality of animal feed and decrease livestock greenhouse gas emissions. Thus, the present study evaluated yield, chemical composition and in vitro gas production of silages produced with intercropped palisade grass (Urochloa brizantha.(A.Rich.) R.D.Webster), pigeon pea (Cajanus cajan cv. Super N) and corn (Zea mays. L.). Forage was harvested and placed inside micro-silos, which were opened after 100 days and samples were collected for chemical composition and in vitro gas production analyses. Intercropped silage had higher crude protein, acid detergent fiber, and lignin content than corn silage. Moreover, intercropped silage decreased total gas and methane production. Therefore, intercropped silage showed potential to increase conserved feed nutritional quality and reduce methane emissions in livestock production systems.

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A comparison between two in vitro gas production techniques to study fermentation profiles of three foodstuffs

BSAP Occasional Publication ◽

10.1017/s0263967x00032614 ◽

1998 ◽

Vol 22 ◽

pp. 207-208

Author(s):

R. S. Lowman ◽

N. S. Jessop ◽

M. K. Theodorou ◽

M. Herrero ◽

D. Cuddeford

Keyword(s):

Pressure Transducer ◽

Rumen Fluid ◽

Gas Production ◽

In Vitro Gas Production ◽

Production Techniques ◽

Gas Tight ◽

Gas Volume ◽

Kinetics Of ◽

Made In

Following the development of the Menke technique in 1979, the measurement of gas production in vitro has become increasingly popular for investigating the kinetics of rumen fermentation. The aim of this study was to compare the gas production profiles for three foods using two in vitro gas production techniques; the Menke et al. (1979) technique (MT) and the pressure transducer technique (PTT) (Theodorou et al., 1994). Both techniques involve recording gas production throughout the incubation of a food sample with rumen fluid. The MT incubations are made in gas-tight syringes where the volume of gas produced causes the plunger to move up the syringe barrel. The PTT involves measuring gas production in fermentation bottles using a pressure transducer and syringe assembly to measure the pressure and corresponding gas volume. As the medium to rumen fluid ratios also differ between techniques; 2:1 in the Menke technique and 9:1 in the PTT, both ratios were investigated in this study.

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Comparison of in situ and in vitro gas production techniques to estimate rumen degradation characteristics of forages

BSAP Occasional Publication ◽

10.1017/s0263967x00032754 ◽

1998 ◽

Vol 22 ◽

pp. 247-248

Author(s):

S. López ◽

M. D. Carro ◽

C. Valdés ◽

J. S. González ◽

F. J. Ovejero

Keyword(s):

Gas Production ◽

Degradation Kinetic ◽

Production Methods ◽

Rumen Degradation ◽

In Vitro Gas Production ◽

Dry Matter Degradation ◽

Production Techniques ◽

Kinetics Of

Quantitative expressions of the kinetics of digestion are needed to estimate more precisely the quantity and composition of nutrients digested from feeds and their subsequent efficiency of utilization by the animal (Mertens, 1993). Degradation kinetic parameters are estimated by fitting appropriate models to data resulting from the measurement of either the undigested residue or the fermentation end-products after exposure of the feed to digestion. Kinetic data can be collected using either in vitro or in situ procedures. The in situ porous synthetic fibre bag technique has been extensively used to describe the kinetics of feeds degradation in the rumen. Several in vitro gas production methods have been developed to assess fermentation kinetics. The aim of this work was to study the relationship between rumen degradation parameters of forages determined either by the in situ procedure or by the gas production technique and to evaluate the potential of gas production measurements to assess the extent of dry matter degradation in the rumen.

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Relationship between in vitro gas production and neutral detergent fibre disappearance in three tropical grasses

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200594113 ◽

1996 ◽

Vol 1996 ◽

pp. 219-219

Author(s):

M. Herrero ◽

N.S. Jessop

Keyword(s):

Cell Wall ◽

Gas Production ◽

Adequate Description ◽

Tropical Grasses ◽

Neutral Detergent Fibre ◽

Early Stages ◽

In Vitro Gas Production ◽

Production Techniques ◽

Gas Volume

In vitro gas production techniques have been used to nutritionally characterise feedstuffs for ruminants. Consideration of both the soluble and insoluble fractions has recently been shown to be essential for adequate description of cell wall disappearance (Jessop and Herrero, 1996). This study investigates how gas production measurements can be used to predict neutral detergent fibre (NDF) disappearance by correcting for the gas volume produced in the early stages of fermentation from neutral detergent solubles (NDS). The study was carried out using three tropical grasses.

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