scholarly journals Potent antiviral activity of brequinar against the emerging Cantagalo virus in cell culture

2011 ◽  
Vol 38 (5) ◽  
pp. 435-441 ◽  
Author(s):  
Laila Castro Schnellrath ◽  
Clarissa R. Damaso
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Potent Antiviral Activity

scholarly journals Importance of Apolipoprotein A-I and A-II Composition in HDL and Its Potential for Studying COVID-19 and SARS-CoV-2

Medicines ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 38
Author(s):  
Kyung-Hyun Cho
Keyword(s):  
Antiviral Activity ◽  
High Density ◽  
High Density Lipoproteins ◽  
Potent Antiviral Activity ◽  
Apolipoprotein A ◽  
Putative Role

The composition and properties of apolipoprotein (apo) A-I and apoA-II in high-density lipoproteins (HDL) might be critical to SARS-CoV-2 infection via SR-BI and antiviral activity against COVID-19. HDL containing native apoA-I showed potent antiviral activity, while HDL containing glycated apoA-I or other apolipoproteins did not. However, there has been no report to elucidate the putative role of apoA-II in the antiviral activity of HDL.

Antiviral activity of uridine 5′-diphosphate glucose analogues against some enveloped viruses in cell culture

1987 ◽  
Vol 8 (5-6) ◽  
pp. 299-310 ◽  
Author(s):  
G. Gil-Fernández ◽  
S. Pérez ◽  
P. Vilas ◽  
C. Pérez ◽  
F.G. de las Heras ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Enveloped Viruses ◽  
Glucose Analogues ◽  
Diphosphate Glucose

Thiazolidinone–Peptide Hybrids as Dengue Virus Protease Inhibitors with Antiviral Activity in Cell Culture

2013 ◽  
Vol 56 (21) ◽  
pp. 8389-8403 ◽  
Author(s):  
Christoph Nitsche ◽  
Verena N. Schreier ◽  
Mira A. M. Behnam ◽  
Anil Kumar ◽  
Ralf Bartenschlager ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Dengue Virus ◽  
Protease Inhibitors

scholarly journals In vitro activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus

2021 ◽  
Vol 66 (2) ◽  
pp. 123-128
Author(s):  
S. Ya. Loginova ◽  
V. N. Shсhukina ◽  
S. V. Savenko ◽  
S. V. Borisevich
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Viral Infections ◽  
Complete Suppression ◽  
Antiviral Efficacy ◽  
Prevention And Treatment ◽  
Proven Efficacy ◽  
High Antiviral Activity

Introduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency of finding drugs effective against this pathogen.The aim of this work was to evaluate the in vitro antiviral efficacy of human recombinant alpha-2b interferon (IFN-α2b) against SARS-CoV-2 virus.Material and methods. The experiments had been carried out on Vero Cl008, the continuous line of African green monkey (Chlorocebus sabaeus) kidney cells. The effectiveness of the drugs was assessed by the suppression of viral reproduction in vitro. The biological activity was determined using titration of a virus-containing suspension in a Vero Cl008 cell culture by the formation of negative colonies.Results. The antiviral efficacy of the IFN-α2b-based medications, which have a high safety profile and proven efficacy in the prevention and treatment of influenza and acute respiratory viral infections (ARVI), has been studied against the new pandemic SARS-CoV-2 virus in vitro experiments in Vero C1008 cell culture. IFN-α2b effectively inhibits the reproduction of the virus when applied both 24 hrs before and 2 hrs after infection. In the IFN-α2b concentration range 102–106 IU/ml a complete suppression of the reproduction of the SARS-CoV-2 virus had been demonstrated.Discussion. IFN-α2b demonstrated in vitro high antiviral activity against SARS-CoV-2. In addition, the substance has a high chemotherapeutic index (>1000).Conclusion. Medications for intranasal use based on IFN-α2b have high antiviral activity and are promising drugs for in vivo study in terms of prevention and treatment of COVID-19.

scholarly journals Inhibition of human cytomegalovirus immediate-early gene expression by an antisense oligonucleotide complementary to immediate-early RNA.

1996 ◽  
Vol 40 (9) ◽  
pp. 2004-2011 ◽  
Author(s):  
K P Anderson ◽  
M C Fox ◽  
V Brown-Driver ◽  
M J Martin ◽  
R F Azad
Keyword(s):  
Gene Expression ◽  
Cell Culture ◽  
Antiviral Activity ◽  
Protein Expression ◽  
Human Cytomegalovirus ◽  
Host Cells ◽  
Early Gene ◽  
Immediate Early ◽  
Mrna Levels ◽  
Virus Adsorption

ISIS 2922 is a phosphorothioate oligonucleotide that is complementary to human cytomegalovirus (CMV) immediate-early (IE) RNA and that exhibits potent and specific antiviral activity against CMV in cell culture assays. Specific assay systems were developed to separately characterize the antisense and nonantisense components of the antiviral activity mediated by ISIS 2922. In U373 cells transformed with cDNA encoding the CMV IE 55-kDa (IE55) protein, expression was inhibited at nanomolar concentrations comparable to effective concentrations in antiviral assays. The specificity of inhibition was demonstrated by using control oligonucleotides incorporating progressive base changes to destabilize oligonucleotide-RNA base pairing and by showing a lack of inhibition of the CMV IE72 product expressed from the same promoter. Inhibition of IE55 protein expression correlated with a reduction in mRNA levels consistent with an RNase H-mediated termination event. Studies with virus-infected cells demonstrated that antisense and nonantisense mechanisms contribute to the antiviral activity of ISIS 2922. Base complementarity to target RNA was important for optimal activity in antiviral assays, but base changes affecting parameters other than hybridization affinity also influenced antiviral activity. Sequence-independent inhibition of virus adsorption to host cells by phosphorothioate oligonucleotides was also observed at high concentrations. Therefore, at least three different mechanisms may contribute to the antiviral activity of ISIS 2922 in cell culture: antisense-mediated inhibition of target gene expression; nonantisense, sequence-dependent inhibition of virus replication; and sequence-independent inhibition of virus adsorption to host cells.

scholarly journals Potent β-Lactam Inhibitors of Human Cytomegalovirus Protease

1998 ◽  
Vol 9 (5) ◽  
pp. 379-387 ◽  
Author(s):  
C Yoakim ◽  
WW Ogilvie ◽  
DR Cameron ◽  
C Chabot ◽  
C Grand-Maître ◽  
...  
Keyword(s):  
Cell Culture ◽  
Antiviral Activity ◽  
Human Cytomegalovirus ◽  
Inhibitory Activity ◽  
Enzymatic Assay ◽  
Side Chain ◽  
Plaque Reduction Assay ◽  
Reduction Assay ◽  
Culture Activity

A series of novel monobactam inhibitors of human cytomegalovirus (HCMV) protease has been described that possess a heterocyclic thiomethyl side chain at C-4. Changes to the heterocycle did not significantly change the inhibitory activity of these compounds in an enzymatic assay, although improvements in solubility and cell culture activity were noted. A number of permutations between C-4 substitutions and N-1 derivatives led to the identification of several β-lactams with antiviral activity in a plaque reduction assay. N-methyl thiotetrazole-containing compounds were found to be the most potent inhibitors in the enzymatic assay.

Modeling the antiviral activity of ribavirin against hepatitis C virus in cell culture

Hepatology ◽  
2013 ◽  
Vol 58 (4) ◽  
pp. 1203-1206
Author(s):  
Daniel J. Felmlee ◽  
Fei Xiao ◽  
Thomas F. Baumert
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antiviral Activity
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