Effects of rice straw structure on chaetoglobosin A production by Chaetomium globosum CGMCC 6882

ABSTRACT Management of nematodes is a very hectic job due to a highly diverse group of organisms. To find lead compounds for new nematicide development, five metabolites (1–5) were isolated from the culture broth of Chaetomium globosum YSC5 and tested for nematicidal activities against the second stage juveniles (J2s) of Meloidogyne javanica. The results revealed that chaetoglobosin A (1), chaetoglobosin B (2) and flavipin (3) exhibited strong adverse effects (91.6, 83.8 and 87.4%, respectively) on J2 mortality at 200 μg/mL with LC50 values of 88.4, 107.7 and 99.2 μg/mL after 72 h, respectively, while 3-methoxyepicoccone (4) and 4,5,6-trihydroxy-7-methylphthalide (5) showed moderate effects (78.0 and 75.5%, respectively) with LC50 values of 124.0 and 131.6 μg/mL, respectively. Furthermore, in pot assay compounds 1 and 2 appeared to be promising metabolites at 200 μg/mL that significantly reduced nematode reproduction and showed a positive influence on plant growth. Our findings could be helpful for development of new potential bio-based pesticides for integrated management of plant-parasitic nematode.

Download Full-text

Heat stability of chaetoglobosins A and C

Canadian Journal of Microbiology ◽

10.1139/w08-024 ◽

2008 ◽

Vol 54 (5) ◽

pp. 423-425 ◽

Cited By ~ 3

Author(s):

M.R. Fogle ◽

D.R. Douglas ◽

C.A. Jumper ◽

D.C. Straus

Keyword(s):

Heat Stability ◽

Chaetomium Globosum ◽

The Future ◽

Chaetoglobosin A

Chaetomium globosum is commonly found in water-damaged buildings and produces the mycotoxins chaetoglobosin A and chaetoglobosin C (Ch-A and Ch-C, respectively). While attempting to purify Ch-A and Ch-C, we observed that these mycotoxins were broken down after heating. The objective of this study was to determine the temperature and the amount of time necessary to break down Ch-A and Ch-C. We demonstrated that the amounts of Ch-A were significantly reduced when exposed to 75 °C for 24 h and 100 °C for 90, 120, or 150 min. Under the same conditions, the levels of Ch-C were also lower (although not significantly). At 175 °C, no Ch-A was detected after 15 min and Ch-C was significantly reduced after 30 min. Our findings will aid other researchers who work with these mycotoxins in the future.

Download Full-text

A Putative C2H2 Transcription Factor CgTF6, Controlled by CgTF1, Negatively Regulates Chaetoglobosin A Biosynthesis in Chaetomium globosum

Frontiers in Fungal Biology ◽

10.3389/ffunb.2021.756104 ◽

2021 ◽

Vol 2 ◽

Author(s):

Yu Yan ◽

Biyun Xiang ◽

Qiaohong Xie ◽

Yamin Lin ◽

Guangya Shen ◽

...

Keyword(s):

Transcription Factor ◽

Gene Cluster ◽

Transcriptional Activation ◽

Chaetomium Globosum ◽

Regulatory Function ◽

Pcr Analysis ◽

Biosynthesis Gene Cluster ◽

Biosynthesis Gene ◽

Chaetoglobosin A ◽

Encoding Genes

Gα signaling pathway as well as the global regulator LaeA were demonstrated to positively regulate the biosynthesis of chaetoglobosin A (ChA), a promising biotic pesticide produced by Chaetomium globosum. Recently, the regulatory function of Zn2Cys6 binuclear finger transcription factor CgcheR that lies within the ChA biosynthesis gene cluster has been confirmed. However, CgcheR was not merely a pathway specific regulator. In this study, we showed that the homologs gene of CgcheR (designated as Cgtf1) regulate ChA biosynthesis and sporulation in C. globosum NK102. More importantly, RNA-seq profiling demonstrated that 1,388 genes were significant differentially expressed as Cgtf1 deleted. Among them, a putative C2H2 transcription factor, named Cgtf6, showed the highest gene expression variation in zinc-binding proteins encoding genes as Cgtf1 deleted. qRT-PCR analysis confirmed that expression of Cgtf6 was significantly reduced in CgTF1 null mutants. Whereas, deletion of Cgtf6 resulted in the transcriptional activation and consequent increase in the expression of ChA biosynthesis gene cluster and ChA production in C. globosum. These data suggested that CgTF6 probably acted as an end product feedback effector, and interacted with CgTF1 to maintain a tolerable concentration of ChA for cell survival.

Download Full-text

Nematicidal Activity of Chaetoglobosin A Poduced by Chaetomium globosum NK102 against Meloidogyne incognita

Journal of Agricultural and Food Chemistry ◽

10.1021/jf304314g ◽

2012 ◽

Vol 61 (1) ◽

pp. 41-46 ◽

Cited By ~ 58

Author(s):

Yang Hu ◽

Weipu Zhang ◽

Ping Zhang ◽

Weibin Ruan ◽

Xudong Zhu

Keyword(s):

Meloidogyne Incognita ◽

Nematicidal Activity ◽

Chaetomium Globosum ◽

Chaetoglobosin A

Download Full-text

Gα-cAMP/PKA pathway positively regulates pigmentation, chaetoglobosin A biosynthesis and sexual development in Chaetomium globosum

PLoS ONE ◽

10.1371/journal.pone.0195553 ◽

2018 ◽

Vol 13 (4) ◽

pp. e0195553 ◽

Cited By ~ 6

Author(s):

Yang Hu ◽

Xiaoran Hao ◽

Longfei Chen ◽

Oren Akhberdi ◽

Xi Yu ◽

...

Keyword(s):

Sexual Development ◽

Chaetomium Globosum ◽

Chaetoglobosin A ◽

Pka Pathway

Download Full-text

ChemInform Abstract: STRUCTURES OF CHAETOGLOBOSIN A AND B, CYTOTOXIC METABOLITES OF CHAETOMIUM GLOBOSUM

Chemischer Informationsdienst ◽

10.1002/chin.197337402 ◽

1973 ◽

Vol 4 (37) ◽

pp. no-no

Author(s):

S. SEKITA ◽

K. YOSHIHIRA ◽

S. NATORI ◽

H. KUWANO

Keyword(s):

Chaetomium Globosum ◽

Chaetoglobosin A

Download Full-text

Functional analysis of a chaetoglobosin A biosynthetic regulator in Chaetomium globosum

Fungal Biology ◽

10.1016/j.funbio.2020.10.010 ◽

2020 ◽

Author(s):

Ming Cheng ◽

Shanshan Zhao ◽

He Liu ◽

Yutao Liu ◽

Congyu Lin ◽

...

Keyword(s):

Functional Analysis ◽

Chaetomium Globosum ◽

Chaetoglobosin A

Download Full-text

Deletion of a Rare Fungal PKS CgPKS11 Promotes Chaetoglobosin A Biosynthesis, Yet Defers the Growth and Development of Chaetomium globosum

Journal of Fungi ◽

10.3390/jof7090750 ◽

2021 ◽

Vol 7 (9) ◽

pp. 750

Author(s):

Biyun Xiang ◽

Xiaoran Hao ◽

Qiaohong Xie ◽

Guangya Shen ◽

Yanjie Liu ◽

...

Keyword(s):

Gene Cluster ◽

Growth Retardation ◽

Growth And Development ◽

Polyketide Synthase ◽

Pathogenic Fungi ◽

Fold Increase ◽

Chaetomium Globosum ◽

Ascospore Development ◽

Chaetoglobosin A ◽

Encoding Genes

We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the biocontrol of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR-Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase encoding genes, Cgpks11, which is putatively involved in the biosynthesis of chaetoglocin A, was disrupted. Cgpks11 deletion led to the overexpression of the CgcheA gene cluster, which is responsible for ChA biosynthesis, and a 1.6-fold increase of ChA. Transcription of pks-1, a melanin PKS, was simultaneously upregulated. Conversely, the transcription of genes for chaetoglocin A biosynthesis, e.g., CHGG_10646 and CHGG_10649, were significantly downregulated. The deletion also led to growth retardation and seriously impaired ascospore development. This study found a novel regulatory means on the biosynthesis of ChA by CgPKS11. CgPKS11 affects chaetoglobosin A biosynthesis, growth, and development in Chaetomium globosum.

Download Full-text