X-ray analysis of chaetoglobosin A, an indol-3-yl-[13]cytochalasan from Chaetomium globosum

1976 ◽  
Vol 17 (17) ◽  
pp. 1349-1350 ◽  
Author(s):  
J.V. Silverton ◽  
T. Akiyama ◽  
C. Kabuto ◽  
S. Sekita ◽  
K. Yoshihira ◽  
...  
Keyword(s):  
Chaetomium Globosum ◽  
X Ray ◽  
Chaetoglobosin A

Nematicidal metabolites from endophytic fungus Chaetomium globosum YSC5

2019 ◽  
Vol 366 (14) ◽  
Author(s):  
Babar Khan ◽  
Wei Yan ◽  
Shan Wei ◽  
Zhiyang Wang ◽  
Shuangshuang Zhao ◽  
...  
Keyword(s):  
Parasitic Nematode ◽  
Integrated Management ◽  
Meloidogyne Javanica ◽  
Positive Influence ◽  
Culture Broth ◽  
Chaetomium Globosum ◽  
Nematode Reproduction ◽  
Lead Compounds ◽  
Second Stage ◽  
Chaetoglobosin A

ABSTRACT Management of nematodes is a very hectic job due to a highly diverse group of organisms. To find lead compounds for new nematicide development, five metabolites (1–5) were isolated from the culture broth of Chaetomium globosum YSC5 and tested for nematicidal activities against the second stage juveniles (J2s) of Meloidogyne javanica. The results revealed that chaetoglobosin A (1), chaetoglobosin B (2) and flavipin (3) exhibited strong adverse effects (91.6, 83.8 and 87.4%, respectively) on J2 mortality at 200 μg/mL with LC50 values of 88.4, 107.7 and 99.2 μg/mL after 72 h, respectively, while 3-methoxyepicoccone (4) and 4,5,6-trihydroxy-7-methylphthalide (5) showed moderate effects (78.0 and 75.5%, respectively) with LC50 values of 124.0 and 131.6 μg/mL, respectively. Furthermore, in pot assay compounds 1 and 2 appeared to be promising metabolites at 200 μg/mL that significantly reduced nematode reproduction and showed a positive influence on plant growth. Our findings could be helpful for development of new potential bio-based pesticides for integrated management of plant-parasitic nematode.

Heat stability of chaetoglobosins A and C

2008 ◽  
Vol 54 (5) ◽  
pp. 423-425 ◽  
Author(s):  
M.R. Fogle ◽  
D.R. Douglas ◽  
C.A. Jumper ◽  
D.C. Straus
Keyword(s):  
Heat Stability ◽  
Chaetomium Globosum ◽  
The Future ◽  
Chaetoglobosin A

Chaetomium globosum is commonly found in water-damaged buildings and produces the mycotoxins chaetoglobosin A and chaetoglobosin C (Ch-A and Ch-C, respectively). While attempting to purify Ch-A and Ch-C, we observed that these mycotoxins were broken down after heating. The objective of this study was to determine the temperature and the amount of time necessary to break down Ch-A and Ch-C. We demonstrated that the amounts of Ch-A were significantly reduced when exposed to 75 °C for 24 h and 100 °C for 90, 120, or 150 min. Under the same conditions, the levels of Ch-C were also lower (although not significantly). At 175 °C, no Ch-A was detected after 15 min and Ch-C was significantly reduced after 30 min. Our findings will aid other researchers who work with these mycotoxins in the future.

scholarly journals MUTANTS PRODUCED BY X-IRRADIATION OF SPORES OF CHAETOMIUM GLOBOSUM AND A COMPARISON WITH THOSE PRODUCED BY ULTRAVIOLET IRRADIATION

1949 ◽  
Vol 32 (6) ◽  
pp. 647-653 ◽  
Author(s):  
J. M. Ford ◽  
D. P. Kirwan
Keyword(s):  
Ultraviolet Irradiation ◽  
Wave Length ◽  
Fungal Spores ◽  
Chaetomium Globosum ◽  
Theoretical Curve ◽  
Striking Difference ◽  
X Rays ◽  
X Ray ◽  
Large Numbers ◽  
X Irradiation

1. Mutants produced by x-irradiation of fungal spores of Chaetomium globosum have been compared with those produced by ultraviolet irradiation. 2. The most striking difference between the mutants produced by x-irradiation and ultraviolet irradiation is the absence in x-ray experiments of the K mutant which is produced in large numbers at short ultraviolet wave lengths. 3. A comparison is made of the relation between x-ray dose and numbers of lethal mutants, and the relation between the short ultraviolet wave length 2804 dose and numbers of lethal mutants. Both are compared with theoretical curves for 1, 2, 5, and 8 quantum hits. 4. The production of lethal mutants by x-rays is shown to be consistent with the theoretical curve for five quantum hits on the sensitive spot of the spore, whereas the production of lethal mutants by the ultraviolet wave length 2804 Å.u. is consistent with two quantum hits.

scholarly journals A Putative C2H2 Transcription Factor CgTF6, Controlled by CgTF1, Negatively Regulates Chaetoglobosin A Biosynthesis in Chaetomium globosum

2021 ◽  
Vol 2 ◽  
Author(s):  
Yu Yan ◽  
Biyun Xiang ◽  
Qiaohong Xie ◽  
Yamin Lin ◽  
Guangya Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Cluster ◽  
Transcriptional Activation ◽  
Chaetomium Globosum ◽  
Regulatory Function ◽  
Pcr Analysis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Chaetoglobosin A ◽  
Encoding Genes

Gα signaling pathway as well as the global regulator LaeA were demonstrated to positively regulate the biosynthesis of chaetoglobosin A (ChA), a promising biotic pesticide produced by Chaetomium globosum. Recently, the regulatory function of Zn2Cys6 binuclear finger transcription factor CgcheR that lies within the ChA biosynthesis gene cluster has been confirmed. However, CgcheR was not merely a pathway specific regulator. In this study, we showed that the homologs gene of CgcheR (designated as Cgtf1) regulate ChA biosynthesis and sporulation in C. globosum NK102. More importantly, RNA-seq profiling demonstrated that 1,388 genes were significant differentially expressed as Cgtf1 deleted. Among them, a putative C2H2 transcription factor, named Cgtf6, showed the highest gene expression variation in zinc-binding proteins encoding genes as Cgtf1 deleted. qRT-PCR analysis confirmed that expression of Cgtf6 was significantly reduced in CgTF1 null mutants. Whereas, deletion of Cgtf6 resulted in the transcriptional activation and consequent increase in the expression of ChA biosynthesis gene cluster and ChA production in C. globosum. These data suggested that CgTF6 probably acted as an end product feedback effector, and interacted with CgTF1 to maintain a tolerable concentration of ChA for cell survival.

scholarly journals Gα-cAMP/PKA pathway positively regulates pigmentation, chaetoglobosin A biosynthesis and sexual development in Chaetomium globosum

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195553 ◽  
Author(s):  
Yang Hu ◽  
Xiaoran Hao ◽  
Longfei Chen ◽  
Oren Akhberdi ◽  
Xi Yu ◽  
...  
Keyword(s):  
Sexual Development ◽  
Chaetomium Globosum ◽  
Chaetoglobosin A ◽  
Pka Pathway

ChemInform Abstract: STRUCTURES OF CHAETOGLOBOSIN A AND B, CYTOTOXIC METABOLITES OF CHAETOMIUM GLOBOSUM

1973 ◽  
Vol 4 (37) ◽  
pp. no-no
Author(s):  
S. SEKITA ◽  
K. YOSHIHIRA ◽  
S. NATORI ◽  
H. KUWANO
Keyword(s):  
Chaetomium Globosum ◽  
Chaetoglobosin A

scholarly journals POTENTIAL OF CELLULASE OF CHAETOMIUM GLOBOSUM FOR PREPARATION AND CHARACTERIZATION OF MICROCRYSTALLINE CELLULOSE FROM WATER HYACINTH (EICHHORNIA CRASSIPES)

2019 ◽  
pp. 140-146
Author(s):  
HERMAN SURYADI ◽  
YULIANITA PRATIWI INDAH LESTARI ◽  
MIRAJUNNISA ◽  
ARRY YANUAR
Keyword(s):  
Particle Size ◽  
Enzymatic Hydrolysis ◽  
Water Hyacinth ◽  
Particle Size Analysis ◽  
Chaetomium Globosum ◽  
Size Analysis ◽  
X Ray Diffraction ◽  
Hydrolysis Time ◽  
X Ray

Objective: This study aimed to increase the yield of microcrystalline cellulose (MCC) made from water hyacinth ɑ-cellulose by enzymatic hydrolysis by using purified enzyme and to find it’s characteristics compared to the reference. Methods: In this research, MCC was prepared from water hyacinth powder through the chemical isolation process of ɑ-cellulose, followed by enzymatic hydrolysis with purified cellulase from Chaetomium globosum. The yield of MCC was improved by using purified enzyme and optimization of temperature, pH, and hydrolysis time. Identification was carried out by using ZnCl and infrared spectrophotometry, followed by characterization of MCC include particle size analysis (PSA) and diffracto­gram pattern (X-Ray Diffraction) compared to reference Avicel PH 101. Results: Purified enzyme from Chaetomium globosum has high activity with a clear zone area of 45 mm with cellulolytic index 6.5 that almost same as Trichoderma reesei (50 mm), with the cellulase enzyme activity of 6.691 U/ml. The optimum condition was at a temperature of 50⁰C and pH 6.0 with the hydrolysis time of 2 h, which produced 95% yield of MCC. Identification with ZnCl and FTIR spectrum showed positive results, similar to the reference. The results of organoleptic test, particle size analysis, and diffracto­gram pattern (X-Ray Diffraction) showed crystalline characteristic similar to reference (Avicel PH 101). Conclusion: Enzyme from Chaetomium globosum has a higher activity of cellulase than Trichoderma reesei with MCC obtained was 95%. Based on the comparison of the organoleptic test, particle size analysis, and diffracto­gram pattern, MCC from water hyacinth has a great potential which showed similar characteristic to reference (Avicel pH 101).

scholarly journals Functional analysis of a chaetoglobosin A biosynthetic regulator in Chaetomium globosum

2020 ◽  
Author(s):  
Ming Cheng ◽  
Shanshan Zhao ◽  
He Liu ◽  
Yutao Liu ◽  
Congyu Lin ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Chaetomium Globosum ◽  
Chaetoglobosin A

scholarly journals Deletion of a Rare Fungal PKS CgPKS11 Promotes Chaetoglobosin A Biosynthesis, Yet Defers the Growth and Development of Chaetomium globosum

2021 ◽  
Vol 7 (9) ◽  
pp. 750
Author(s):  
Biyun Xiang ◽  
Xiaoran Hao ◽  
Qiaohong Xie ◽  
Guangya Shen ◽  
Yanjie Liu ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Growth Retardation ◽  
Growth And Development ◽  
Polyketide Synthase ◽  
Pathogenic Fungi ◽  
Fold Increase ◽  
Chaetomium Globosum ◽  
Ascospore Development ◽  
Chaetoglobosin A ◽  
Encoding Genes

We previously reported that chaetoglobosin A (ChA) exhibits a great potential in the biocontrol of nematodes and pathogenic fungi. To improve the production of ChA, a CRISPR-Cas9 system was created and applied for eliminating potential competitive polyketide products. One of the polyketide synthase encoding genes, Cgpks11, which is putatively involved in the biosynthesis of chaetoglocin A, was disrupted. Cgpks11 deletion led to the overexpression of the CgcheA gene cluster, which is responsible for ChA biosynthesis, and a 1.6-fold increase of ChA. Transcription of pks-1, a melanin PKS, was simultaneously upregulated. Conversely, the transcription of genes for chaetoglocin A biosynthesis, e.g., CHGG_10646 and CHGG_10649, were significantly downregulated. The deletion also led to growth retardation and seriously impaired ascospore development. This study found a novel regulatory means on the biosynthesis of ChA by CgPKS11. CgPKS11 affects chaetoglobosin A biosynthesis, growth, and development in Chaetomium globosum.

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