A comparative study of sulfated tara gum: RSM optimization and structural characterization

2020 ◽  
Vol 150 ◽  
pp. 189-199 ◽  
Author(s):  
Xiaojie Qin ◽  
Rui Li ◽  
Shengyong Zhu ◽  
Jiahuan Hu ◽  
Xiaorong Zeng ◽  
...  
Keyword(s):  
Comparative Study ◽  
Structural Characterization ◽  
Rsm Optimization

scholarly journals Kinetic and structural characterization of the self-labeling protein tags HaloTag7, SNAP-tag and CLIP-tag

2021 ◽  
Author(s):  
Jonas Wilhelm ◽  
Stefanie Kuehn ◽  
Miroslaw Tarnawski ◽  
Guillaume Gotthard ◽  
Jana Tuennermann ◽  
...  
Keyword(s):  
Comparative Study ◽  
Structural Characterization ◽  
Fusion Proteins ◽  
The Self ◽  
Substrate Specificities ◽  
Substrate Preferences ◽  
Diffusion Limited ◽  
Protein Tags ◽  
Selection Of

The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag and CLIP-tag allow the covalent label-ing of fusion proteins with synthetic molecules for applications in bioimaging and biotechnology. To guide the selection of an SLP-substrate pair and provide guidelines for the design of sub-strates, we report a systematic and comparative study on the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag and CLIP-tag. HaloTag7 reaches almost diffusion-limited labeling rates with certain rhodamine substrates, which are more than two orders of magnitude higher than those of SNAP-tag for the corresponding substrates. SNAP-tag labeling rates how-ever are less affected by the structure of the label than those of HaloTag7, which vary over six orders of magnitude for commonly employed substrates. Solving the crystal structures of Halo-Tag7 and SNAP-tag labeled with fluorescent substrates allowed us to rationalize their substrate preferences. We also demonstrate how these insights can be exploited to design substrates with improved labeling kinetics.

Physicochemical and structural characterization of iron–sucrose formulations: a comparative study

2013 ◽  
Vol 19 (5) ◽  
pp. 513-520 ◽  
Author(s):  
Bhavesh S. Barot ◽  
Punit B. Parejiya ◽  
Dharmik M. Mehta ◽  
Pragna K. Shelat ◽  
Gaurang B. Shah
Keyword(s):  
Comparative Study ◽  
Structural Characterization ◽  
Iron Sucrose

scholarly journals Structural Characterization and Mechanical Performance of Calcium Phosphate Scaffolds and Natural Bones: A Comparative Study

2010 ◽  
Vol 8 (3) ◽  
pp. 159-165 ◽  
Author(s):  
Elena Fuentes ◽  
Virginia Sáenz de Viteri ◽  
Amaya Igartua ◽  
Roberta Martinetti ◽  
Laura Dolcini ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Comparative Study ◽  
Structural Characterization ◽  
Mechanical Performance
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