Effects of oxidative modification on the functional, conformational and gelling properties of myofibrillar proteins from Culter alburnus

2020 ◽  
Vol 162 ◽  
pp. 1442-1452 ◽  
Author(s):  
Zhongli Zhang ◽  
Zhouyi Xiong ◽  
Sufang Lu ◽  
Noman Walayat ◽  
Chun Hu ◽  
...  
Keyword(s):  
Oxidative Modification ◽  
Myofibrillar Proteins ◽  
Gelling Properties ◽  
Culter Alburnus

scholarly journals Role of Ovalbumin/β-Cyclodextrin in Improving Structural and Gelling Properties of Culter alburnus Myofibrillar Proteins during Frozen Storage

2021 ◽  
Vol 11 (24) ◽  
pp. 11815
Author(s):  
Meiwen Lv ◽  
Xiukang Wang ◽  
Noman Walayat ◽  
Zhongli Zhang ◽  
Asad Nawaz ◽  
...  
Keyword(s):  
Structural Changes ◽  
Loss Modulus ◽  
Frozen Storage ◽  
Surface Hydrophobicity ◽  
Control Group ◽  
Myofibrillar Proteins ◽  
Gelling Properties ◽  
Seafood Products ◽  
Culter Alburnus ◽  
Industrial Level

This study aimed to analyze the cryoprotective effect of a ovalbumin (OVA) and β-cyclodextrin (βCD) mixture (3:1, OVA/βCD) on the structure, rheology and gelling properties of myofibrillar proteins (MPs) during 90 days of frozen storage. A mixture of OVA/βCD at different concentrations (0, 2, 4, and 6%) was added to MPs and stored at −18 °C for 90 days. The addition of OVA/βCD significantly decreased the sulfhydryl contents while it increased the surface hydrophobicity, which was closely connected with tertiary structural changes. Circular dichroism analysis showed that the addition of OVA/βCD enhanced the stability of the secondary structure by inhibiting the decline in the α-helix. Rheological properties analysis indicated that 6% OVA/βCD treatment showed better storage modulus (G’) and loss modulus (G”). In addition, treatment of OVA/βCD showed better gel forming properties than the control group (0%), helping to form a homogeneous and denser gel network. The results proved that 6% OVA/βCD could be act as a promising cryoprotectant, which can improve the structure and gel behavior of Culter alburnus MPs during frozen storage. Moreover, OVA/βCD could be a potential alternative to conventional cryoprotectants at the industrial level to increase the economic and commercial values of seafood products.

Influence of sodium pyrophosphate on the physicochemical and gelling properties of myofibrillar proteins under hydroxyl radical-induced oxidative stress

2020 ◽  
Vol 11 (3) ◽  
pp. 1996-2004 ◽  
Author(s):  
Yungang Cao ◽  
Wenhui Ma ◽  
Jiankang Wang ◽  
Shuhua Zhang ◽  
Zhenyu Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydroxyl Radical ◽  
Rheological Behavior ◽  
Sodium Pyrophosphate ◽  
Myofibrillar Proteins ◽  
Gelling Properties

Sodium pyrophosphate (PP) addition changed the rheological behavior and improved the gelling properties of oxidative stressed myofibrillar proteins (MP).

Effects of oxidative modification on textural properties and gel structure of pork myofibrillar proteins

2019 ◽  
Vol 121 ◽  
pp. 678-683 ◽  
Author(s):  
Minquan Xia ◽  
Yinxia Chen ◽  
Juanjuan Guo ◽  
Xiaolong Feng ◽  
Xiaoli Yin ◽  
...  
Keyword(s):  
Textural Properties ◽  
Oxidative Modification ◽  
Myofibrillar Proteins ◽  
Gel Structure

scholarly journals Emulsifying and gelling properties of weakfish myofibrillar proteins as affected by squid mantle myofibrillar proteins in a model system

2014 ◽  
Vol 17 (1) ◽  
pp. 8-18 ◽  
Author(s):  
Daniela Mariel Suarez ◽  
Emilio Manca ◽  
Marcos Crupkin ◽  
Maria Elida Paredi
Keyword(s):  
Thermal Treatment ◽  
Functional Properties ◽  
Profile Analysis ◽  
Muscle Protein ◽  
Activity Index ◽  
Gel Strength ◽  
Myofibrillar Proteins ◽  
Gelling Properties ◽  
Water Holding

The aim of the present work was to investigate the physicochemical, biochemical and functional characteristics of both the myofibrils (MF) and actomyosin (AM) of squid mantle (Illex argentinus) and weakfish (Cynoscion guatucupa) muscles, and evaluate the influence of the addition of myofibrilar proteins from the squid mantle on the physicochemical and functional properties of those of the weakfish. After extraction, purification and characterization of the MF and AM of both species, emulsions of each protein fraction from each muscle were formulated. Mixtures of the MF or AM of both species were also analyzed. The emulsifying properties were monitoring using the Emulsifying Activity Index (EAI) and Emulsion Stability (ES). In addition, gel pastes were formulated from the squid mantle, weakfish muscle and the mixture of both species, and the following functional properties of the gels assessed: water holding capacity, colour, textural profile analysis (TPA) (hardness, elasticity, cohesiveness, gumminess) and gel strength. The EAI values of emulsions formulated with the MF of the mantle were significantly (p<0.05) higher than those formulated from those of weakfish. The incorporation of squid MF in the mixture increased the EAI values. Conversely, the highest ES values were obtained with weakfish MF, and the incorporation of MF weakfish in the mixture increased the ES values. Similar EAI and ES behaviours were observed for the AM of the corresponding species. Irrespective of the thermal treatment, the gel strength of the gelled paste of squid muscle was significantly (p<0.05) lower than that of weakfish muscle and of those obtained with the different mixtures. The behaviours of the expressible moisture (EM) from the gelled pastes were similar to those of gel strength. Irrespective of the thermal treatment, the pastes formulated with a high weakfish: mantle ratio showed less water loss. The gelled pastes of squid mantle showed the highest values for whiteness (WI) and the incorporation of squid muscle protein improved the WI of the mixtures.

Myofibrillar protein oxidation and contractile dysfunction in hyperthyroid rat diaphragm

2007 ◽  
Vol 102 (5) ◽  
pp. 1850-1855 ◽  
Author(s):  
Takashi Yamada ◽  
Takaaki Mishima ◽  
Makoto Sakamoto ◽  
Minako Sugiyama ◽  
Satoshi Matsunaga ◽  
...  
Keyword(s):  
Protein Oxidation ◽  
Force Production ◽  
Oxidative Modification ◽  
Fiber Bundles ◽  
Daily Injection ◽  
Myofibrillar Proteins ◽  
Carbonyl Groups ◽  
Carbonyl Content ◽  
Tetanic Force ◽  
Isometric Forces

The purpose of the present study was to test the hypothesis that administration of thyroid hormone [3,5,3′-triiodo-l-thyronine (T3)] could result in oxidation of myofibrillar proteins and, in turn, induce alterations in respiratory muscle function. Daily injection of T3 for 21 days depressed isometric forces of diaphragm fiber bundles across a range of stimulus frequencies (1, 10, 20, 40, 75, and 100 Hz) ( P < 0.05). These reductions in force production were accompanied by a remarkable increment (104%; P < 0.05) in carbonyl groups of myofibrillar proteins. In contrast, T3 treatment has no effects on the carbonyl content in myosin heavy chain. In additional experiments, we have also tested the efficacy of carvedilol, a nonselective β1- β2-blocker that possesses antioxidative properties. Treatment with carvedilol dramatically improved isometric tetanic force production at stimulus frequencies from 40 to 100 Hz ( P < 0.05). Carvedilol also prevented T3-induced contractile protein oxidation ( P < 0.05). These data suggest that the oxidative modification of myofibrillar proteins may account, at least in part, for an impairment of diaphragm in hyperthyroidism.

Sign in / Sign up

Export Citation Format

Share Document