Ag@MOF-loaded p-coumaric acid modified chitosan/chitosan nanoparticle and polyvinyl alcohol/starch bilayer films for food packing applications

2022 ◽  
Author(s):  
Meng Zhang ◽  
Yuqi Zheng ◽  
Yang Jin ◽  
Dong Wang ◽  
Guohui Wang ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Coumaric Acid ◽  
Modified Chitosan ◽  
Bilayer Films ◽  
Chitosan Nanoparticle

scholarly journals Effect of Sonication Duration in the Performance of Polyvinyl Alcohol/Chitosan Bilayer Films and Their Effect on Strawberry Preservation

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1408 ◽  
Author(s):  
Jie Ding ◽  
Rong Zhang ◽  
Saeed Ahmed ◽  
Yaowen Liu ◽  
Wen Qin
Keyword(s):  
Polyvinyl Alcohol ◽  
Water Vapor Permeability ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Solid Content ◽  
Bilayer Film ◽  
Vapor Permeability ◽  
Soluble Solid Content ◽  
Health Food ◽  
Bilayer Films

In this study, we fabricated polyvinyl alcohol (PVA)/chitosan (CS) bilayer films by casting and investigated the effects of preparation conditions and CS content (2, 2.5, or 3 wt.%) on the ability of these films to preserve packaged strawberries. The best performance was achieved at a CS loading of 2.5 wt.% (ultrasound time, 25 min); the strain and stress values were 143.15 ± 6.43% and 70.67 ± 0.85 MPa, respectively, oxygen permeability was 0.16 ± 0.08 cm2·m2·day−1·MPa−1, water vapor permeability was 14.93 ± 4.09 g·cm−1·s−1·Pa−1, and the shelf life of fresh strawberries packaged in the PVA/CS 2.5 wt.% bilayer film was determined to be 21 days at 5 ± 2 °C and a relative humidity of 60 ± 5%. Treatment with PVA/CS bilayer films prevented the decrease in the firmness of strawberries during storage (21 days). The evaluated physicochemical parameters (weight loss, decay, firmness, titratable acidity, soluble solid content, ascorbic acid content, and color) indicated that treatment with PVA/CS bilayer films led to better maintenance of the fruit quality. We believe that our study makes a significant contribution to literature because it paves the way to the fabrication of smart packaging materials and facilitates the commercialization of fresh strawberries as an important health food.

Preparation of a Modified Chitosan-Mycelium Adsorbent with Polyvinyl Alcohol

2014 ◽  
Vol 49 (8) ◽  
pp. 1279-1288 ◽  
Author(s):  
Gang Xiao ◽  
Kai Lan ◽  
Haijia Su ◽  
Tianwei Tan
Keyword(s):  
Polyvinyl Alcohol ◽  
Modified Chitosan

scholarly journals Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers

Vaccines ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 299
Author(s):  
Yi Han ◽  
Sankar Renu ◽  
Veerupaxagouda Patil ◽  
Jennifer Schrock ◽  
Ninoshkaly Feliciano-Ruiz ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Outer Membrane Proteins ◽  
Live Vaccine ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Modified Chitosan ◽  
Mucosal Antibody ◽  
Chitosan Nanoparticle ◽  
Cellular Immune ◽  
Huge Challenge

Controlling Salmonella enterica serovar Enteritidis (SE) infection in broilers is a huge challenge. In this study, our objective was to improve the efficacy of a chitosan nanoparticle (CS)-based Salmonella subunit vaccine for SE, containing immunogenic outer membrane proteins (OMP) and flagellin (FLA), called the CS(OMP+FLA) vaccine, by surface conjugating it with mannose to target dendritic cells, and comparing the immune responses and efficacy with a commercial live Salmonella vaccine in broilers. The CS(OMP+FLA)-based vaccines were administered orally at age 3 days and as a booster dose after three weeks, and the broilers were challenged with SE at 5 weeks of age. Birds were sacrificed 10 days post-challenge and it was observed that CS(OMP+FLA) vaccine surface conjugated with both mannose and FLA produced the greatest SE reduction, by over 1 log10 colony forming unit per gram of the cecal content, which was comparable to a commercial live vaccine. Immunologically, specific mucosal antibody responses were enhanced by FLA-surface-coated CS(OMP+FLA) vaccine, and mannose-bound CS(OMP+FLA) improved the cellular immune response. In addition, increased mRNA expression of Toll-like receptors and cytokine was observed in CS(OMP+FLA)-based-vaccinated birds. The commercial live vaccine failed to induce any such substantial immune response, except that they had a slightly improved T helper cell frequency. Our data suggest that FLA-coated and mannose-modified CS(OMP+FLA) vaccine induced robust innate and adaptive cell-mediated immune responses and substantially reduced the Salmonella load in the intestines of broilers.

Poloxamer Modified Chitosan Nanoparticles for Vaginal Delivery of Acyclovir

2021 ◽  
Vol 08 ◽  
Author(s):  
Sanjeevani Deshkar ◽  
Sumit Sikchi ◽  
Anjali Thakre ◽  
Rupali Kale
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Sodium Tripolyphosphate ◽  
Chitosan Nanoparticles ◽  
Drug Uptake ◽  
Mass Ratio ◽  
Modified Chitosan ◽  
Chitosan Nanoparticle ◽  
Nanoparticle Formulation

Objective: The aim of the present study was to design a surface modified chitosan nanoparticle system for vaginal delivery of Acyclovir for effective drug uptake into vaginal mucosa. Method: Acyclovir loaded chitosan nanoparticles, with and without modification by poloxamer 407, were prepared by ionic gelation method. The effects of two independent variables, chitosan to sodium tripolyphosphate mass ratio (X1) and acyclovir concentration (X2), on drug entrapment in nanoparticles, were studied using 32 full factorial design. The surface response and counter plots were drawn to facilitate an understanding of the contribution of the variables and their interaction. The nanoparticles were evaluated for drug entrapment, size with zeta potential, morphological analysis by TEM, solid state characterization by FTIR, DSC, XRD, in vitro dissolution, in vitro cell uptake using HeLa cell line and in vivo vaginal irritation test in Wistar rats. Results: Chitosan nanoparticle formulation with chitosan to sodium tripolyphosphate mass ratio of 2:1 and acyclovir concentration of 2 mg/mL resulted in highest entrapment efficiency. Resulting nanoparticles revealed spherical morphology with particle size of 191.2 nm. The surface modification of nanoparticles with Poloxamer resulted in higher drug entrapment (74.3±1.5%), higher particle size (391.1 nm) as a result of dense surface coating, lower zeta potential and sustained drug release compared to unmodified nanoparticles. The change in the crystallinity of drug during nanoparticle formulation was observed in DSC and XRD study. Cellular uptake of Poloxamer modified chitosan nanoparticles was found to be higher than chitosan nanoparticles in HeLa cells. Safety of nanoparticle formulations by vaginal route was evident when tested in female rats. Conclusion: Conclusively, Poloxamer modified CH NP could serve as a promising and safe delivery system with enhanced cellular drug uptake.

Chitosan nanoparticle carrier based on surface molecularly imprinted polymers for the recognition and separation of proteins

RSC Advances ◽  
2015 ◽  
Vol 5 (128) ◽  
pp. 106197-106205 ◽  
Author(s):  
Cenjin Zhang ◽  
Yuzhi Wang ◽  
Junxia Guo ◽  
Yanjin Liu ◽  
Yigang Zhou
Keyword(s):  
Aqueous Solution ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Molecularly Imprinted Polymers ◽  
Bovine Serum ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Modified Chitosan ◽  
Chitosan Nanoparticle ◽  
Separation Of Proteins

This paper discusses the construction of surface molecularly imprinted polymers (MIPs) based on modified chitosan (CS) nanoparticle carriers to recognize and separate bovine serum albumin (BSA, pI 4.9, MW 69.0 kDa) in aqueous solution.

scholarly journals Development and Optimization of Chitosan Nanoparticle-Based Intranasal Vaccine Carrier

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 204
Author(s):  
Xiaoyi Gao ◽  
Nan Liu ◽  
Zengming Wang ◽  
Jing Gao ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Vaccine Delivery ◽  
Lavage Fluid ◽  
In Vivo Studies ◽  
Modified Chitosan ◽  
Chitosan Nanoparticle ◽  
Low Toxicity ◽  
Intranasal Vaccine

Chitosan is a natural polysaccharide, mainly derived from the shell of marine organisms. At present, chitosan has been widely used in the field of biomedicine due to its special characteristics of low toxicity, biocompatibility, biodegradation and low immunogenicity. Chitosan nanoparticles can be easily prepared. Chitosan nanoparticles with positive charge can enhance the adhesion of antigens in nasal mucosa and promote its absorption, which is expected to be used for intranasal vaccine delivery. In this study, we prepared chitosan nanoparticles by a gelation method, and modified the chitosan nanoparticles with mannose by hybridization. Bovine serum albumin (BSA) was used as the model antigen for development of an intranasal vaccine. The preparation technology of the chitosan nanoparticle-based intranasal vaccine delivery system was optimized by design of experiment (DoE). The DoE results showed that mannose-modified chitosan nanoparticles (Man-BSA-CS-NPs) had high modification tolerance and the mean particle size and the surface charge with optimized Man-BSA-CS-NPs were 156 nm and +33.5 mV. FTIR and DSC results confirmed the presence of Man in Man-BSA-CS-NPs. The BSA released from Man-BSA-CS-NPs had no irreversible aggregation or degradation. In addition, the analysis of fluorescence spectroscopy of BSA confirmed an appropriate binding constant between CS and BSA in this study, which could improve the stability of BSA. The cell study in vitro demonstrated the low toxicity and biocompatibility of Man-BSA-CS-NPs. Confocal results showed that the Man-modified BSA-FITC-CS-NPs promote the endocytosis and internalization of BSA-FITC in DC2.4 cells. In vivo studies of mice, Man-BSA-CS-NPs intranasally immunized showed a significantly improvement of BSA-specific serum IgG response and the highest level of BSA-specific IgA expression in nasal lavage fluid. Overall, our study provides a promising method to modify BSA-loaded CS-NPs with mannose, which is worthy of further study.

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