Use of PCR primers derived from a putative transcriptional regulator gene for species-specific determination of Listeria monocytogenes

2004 ◽  
Vol 91 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Dongyou Liu ◽  
A.Jerald Ainsworth ◽  
Frank W. Austin ◽  
Mark L. Lawrence
Keyword(s):  
Listeria Monocytogenes ◽  
Transcriptional Regulator ◽  
Pcr Primers ◽  
Regulator Gene ◽  
Specific Determination ◽  
Species Specific

scholarly journals Development of PCR for specific determination of root-knot nema­tode Meloidogyne incognita

2011 ◽  
Vol 39 (No. 1) ◽  
pp. 23-28 ◽  
Author(s):  
B. Tesařová ◽  
M. Zouhar ◽  
P. Ryšánek
Keyword(s):  
Meloidogyne Incognita ◽  
Gene Sequence ◽  
Pcr Primers ◽  
Root Knot Nematode ◽  
Specific Pcr ◽  
Spiked Soil ◽  
Specific Determination ◽  
Species Specific ◽  
Different Origins

PCR primers designed from the gene sequence for the SEC 1 oesophageal gland protein were used to specifically detect and differentiate the root-knot nematode Meloidogyne incognita from other species from the genus Meloido­gyne. Amplification products were obtained from five M. incognita populations from different origins whereas DNA from M. fallax, M. javanica, M. arenaria, M. chitwoodi and M. hapla was not amplified. DNA extracted from different materials (females, root galls and spiked soil) could easily be used for M. incognita detection. One female gave sufficient amount of DNA for detection. Together with mitochondrial DNA this is one of the first attempts to use a gene outside of ITS regions for species specific PCR in the genus Meloidogyne.  

scholarly journals A new method for rapid detection of Listeria monocytogenes in food

2000 ◽  
Vol 18 (No. 3) ◽  
pp. 103-109 ◽  
Author(s):  
K. Zdeňková ◽  
K. Demnerová ◽  
G. Jeníková ◽  
J. Pazlarová
Keyword(s):  
Listeria Monocytogenes ◽  
Pcr Primers ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Special Importance ◽  
Ground Meat ◽  
Specific Detection ◽  
Gene Encoding ◽  
Pcr Method

Listeria monocytogenes represents serious danger for human health. Thus detection of this pathogen in food, which represents its main means of entry into the organism, is a topic of special importance. The original classic methods for the determination of Listeria monocytogenes are in general laborious and time-consuming procedures. In order to address this issue we developed a new rapid method for specific detection of Listeria monocytogenes in food samples. The method consists of three steps: i) enrichment of food microflora (16 h), ii) selective isolation of Listeria sp. exploiting immunomagnetic separation (2–3 h) followed by iii) precise identification of Listeria sp. and Listeria monocytogenes using duplex PCR. PCR primers specific to part of 16S rRNA were used in order to identify the members of Listeria genus. The specific identification of Listeria monocytogenes was accomplished exploiting a pair of primers specific to gene encoding invasion-associated protein – iap (4–5 h). Amplification products, 1003 bp and 593 bp respectively, were separated by electrophoresis and visualized by UV detection. The optimized IMS-PCR method was used to test the presence of Listeria sp. and Listeria monocytogenes in food samples (ground meat, low-fat milk and cheese [olomoucké tvarůžky]). A comparison of the efficiency of the bacteria enrichment step by IMS and centrifugation was also performed. The analysis time including enrichment is less than 24 h. The detection limit for Listeria monocytogenes was found between 101–102 cfu per 25 g of food sample.

A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes

2007 ◽  
Vol 71 (2) ◽  
pp. 133-140 ◽  
Author(s):  
Dongyou Liu ◽  
Mark L. Lawrence ◽  
Frank W. Austin ◽  
A. Jerald Ainsworth
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Specific Determination

Molecular activation analysis for methylmercury

1978 ◽  
Vol 56 (14) ◽  
pp. 1956-1960 ◽  
Author(s):  
J.-P. Schmit ◽  
D. R. Wiles
Keyword(s):  
Activation Analysis ◽  
Methyl Groups ◽  
Specific Determination ◽  
Unidentified Component ◽  
Species Specific ◽  
Atom Chemistry ◽  
Molecular Activation

The principle of molecular activation analysis is described, as well as its unsuccessful application to the species specific determination of methylmercury in fish. The reasons for the failure of the method were determined and are found to be of significance to hot atom chemistry and to possible future use of this principle. The reasons are: (i) a matrix dilution of the species sought, (ii) a scavenging, probably of methyl groups, by serine or related components of fish powder, and (iii) a methylation by some unidentified component associated with the fish fats.

Rapid identification of Streptococcus pyogenes with PCR primers from a putative transcriptional regulator gene

2005 ◽  
Vol 156 (4) ◽  
pp. 564-567 ◽  
Author(s):  
Dongyou Liu ◽  
Susan Hollingshead ◽  
Edwin Swiatlo ◽  
Mark L. Lawrence ◽  
Frank W. Austin
Keyword(s):  
Streptococcus Pyogenes ◽  
Transcriptional Regulator ◽  
Pcr Primers ◽  
Rapid Identification ◽  
Regulator Gene

PCR detection of pathogenic Leptospira genomospecies targeting putative transcriptional regulator genes

2006 ◽  
Vol 52 (3) ◽  
pp. 272-277 ◽  
Author(s):  
Dongyou Liu ◽  
Mark L Lawrence ◽  
Frank W Austin ◽  
A Jerald Ainsworth ◽  
Lanny W Pace
Keyword(s):  
Molecular Mechanisms ◽  
Control Strategies ◽  
Transcriptional Regulator ◽  
Diagnostic Procedures ◽  
Pcr Primers ◽  
Regulator Gene ◽  
Pathogenic Leptospira ◽  
Pathogenic Potential ◽  
Diagnostic Potential ◽  
Regulator Genes

The genus Leptospira comprises multiple genomospecies that demonstrate varied pathogenic potential. The availability of rapid and precise diagnostic procedures to differentiate pathogenic from nonpathogenic Leptospira spp. is therefore essential to prevent an otherwise easily treatable malaise from developing into a life-threatening disease. In this report, we conducted an investigation on the diagnostic potential of Leptospira genes encoding putative tran scriptional regulators. While PCR primers derived from transcriptional regulator gene la1137 recognized all 24 pathogenic Leptospira strains representing seven species, those from la1937, la3231, la3825, and la4130 detected 19 of the 24 Leptospira strains. However, none of these primers reacted with four nonpathogenic Leptospira species or other common bacteria. The putative transcriptional regulator genes la1137, la1937, la3231, la3825, and la4130 are present in pathogenic Leptospira strains, making them potential targets for diagnostic applications. Further characterization of these genes and their proteins may help elucidate the molecular mechanisms of leptospiral virulence and pathogenicity and pave the way for potential development of novel control strategies against leptospirosis.Key words: Leptospira, pathogenic, transcriptional regulator gene, PCR, identification.

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