Phylogenetic analysis of glycoprotein (G) gene of rabies virus isolated from spotted deer, Delhi, 2016

The article presents a molecular genetic study of genomes of field isolates of rabies virus isolated in the Vladimir, Moscow, Tver, Nizhny Novgorod and Ryazan regions, with the aim of carrying out phylogenetic analysis. We studied 20 samples of purified PCR products containing the rabies virus nucleoprotein. The samples were provided by the Vladimir veterinary service. Sequencing and phylogenetic analysis of the gene showed that 12 fragments of isolates under study were close to the Central phylogenetic group of the rabies virus; namely - 5 isolates from the Vladimir region, 2 from the Nizhny Novgorod region, 2 from the Moscow region, and 3 from the Tver region. Eight studied isolates from the Nizhny Novgorod and Ryazan regions were attributed to the Eurasian phylogenetic group.

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Phylogenetic analysis of near full-length sequences of the Desmodus rotundus genetic lineage of rabies virus

Infection Genetics and Evolution ◽

10.1016/j.meegid.2020.104179 ◽

2020 ◽

Vol 80 ◽

pp. 104179

Author(s):

Angélica Cristine de Almeida Campos ◽

Camila Malta Romano ◽

Fernando Lucas Melo ◽

Danielle Bastos Araújo ◽

Elenice Maria Sequetin Cunha ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Rabies Virus ◽

Full Length ◽

Genetic Lineage ◽

Desmodus Rotundus

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Deficient Incorporation of Rabies Virus Glycoprotein into Virions Enhances Virus-Induced Immune Evasion and Viral Pathogenicity

Viruses ◽

10.3390/v11030218 ◽

2019 ◽

Vol 11 (3) ◽

pp. 218 ◽

Cited By ~ 2

Author(s):

Chunfu Li ◽

Hongliang Zhang ◽

Lina Ji ◽

Xiao Wang ◽

Yongjun Wen ◽

...

Keyword(s):

Immune Response ◽

Rabies Virus ◽

Recombinant Virus ◽

Neutralizing Antibodies ◽

Single Copy ◽

Wild Type ◽

Antiviral Immune Response ◽

Glycoprotein G ◽

Lethal Infection ◽

Infected Cells

Previous studies have shown that wild-type (wt) rabies virus (RABV) evades the host immune response by restricting expression of glycoprotein (G), which blocks activation of dendritic cells (DCs) and induces production of virus-neutralizing antibodies (VNAs). In the present study, wt RABVs not only restricted G expression but also reduced incorporation of G into mature virions compared with laboratory-adapted viruses. A recombinant RABV expressing triple G was used to further determine whether G expression relates to incorporation. The recombinant virus showed higher expression and incorporation of G and activated more DCs than the virus that expressed a single copy of G. Removal of G from viruses using subtilisin or Dithiothreitol (DTT)/ Nonidet P-40 (NP40) almost completely abolishes DC activation and VNA production. Consequently, these G-depleted viruses cause lethal infection in mice. Thus, wt RABVs can subvert DC-induced antiviral immune response and maintain pathogenicity by decreasing G expression in infected cells and G incorporation into virions.

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Typing of the rabies virus in Chile, 2002–2008

Epidemiology and Infection ◽

10.1017/s0950268812000520 ◽

2012 ◽

Vol 140 (12) ◽

pp. 2157-2162 ◽

Cited By ~ 7

Author(s):

V. YUNG ◽

M. FAVI ◽

J. FERNANDEZ

Keyword(s):

Phylogenetic Analysis ◽

Monoclonal Antibodies ◽

Rabies Virus ◽

Tadarida Brasiliensis ◽

Insectivorous Bats ◽

Nucleoprotein Gene ◽

Nucleoprotein N

SUMMARYIn Chile, dog rabies has been controlled and insectivorous bats have been identified as the main rabies reservoir. This study aimed to determine the rabies virus (RABV) variants circulating in the country between 2002 and 2008. A total of 612 RABV isolates were tested using a panel with eight monoclonal antibodies against the viral nucleoprotein (N-mAbs) for antigenic typing, and a product of 320-bp of the nucleoprotein gene was sequenced from 99 isolates. Typing of the isolates revealed six different antigenic variants but phylogenetic analysis identified four clusters associated with four different bat species. Tadarida brasiliensis bats were confirmed as the main reservoir. This methodology identified several independent rabies enzootics maintained by different species of insectivorous bats in Chile.

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