The diagnostic value of polymerase chain reaction for ocular tuberculosis diagnosis in relation to antitubercular therapy response: a meta-analysis

AbstractNowadays there is an ongoing acute respiratory outbreak caused by the novel highly contagious coronavirus (COVID-19). The diagnostic protocol is based on quantitative reverse-transcription polymerase chain reaction (RT-PCR) and chests CT scan, with uncertain accuracy. This meta-analysis study determines the diagnostic value of an initial chest CT scan in patients with COVID-19 infection in comparison with RT-PCR. Three main databases; PubMed (MEDLINE), Scopus, and EMBASE were systematically searched for all published literature from January 1st, 2019, to the 21st May 2020 with the keywords "COVID19 virus", "2019 novel coronavirus", "Wuhan coronavirus", "2019-nCoV", "X-Ray Computed Tomography", "Polymerase Chain Reaction", "Reverse Transcriptase PCR", and "PCR Reverse Transcriptase". All relevant case-series, cross-sectional, and cohort studies were selected. Data extraction and analysis were performed using STATA v.14.0SE (College Station, TX, USA) and RevMan 5. Among 1022 articles, 60 studies were eligible for totalizing 5744 patients. The overall sensitivity, specificity, positive predictive value, and negative predictive value of chest CT scan compared to RT-PCR were 87% (95% CI 85–90%), 46% (95% CI 29–63%), 69% (95% CI 56–72%), and 89% (95% CI 82–96%), respectively. It is important to rely on the repeated RT-PCR three times to give 99% accuracy, especially in negative samples. Regarding the overall diagnostic sensitivity of 87% for chest CT, the RT-PCR testing is essential and should be repeated to escape misdiagnosis.

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Performance of Polymerase Chain Reaction Analysis of the Amniotic Fluid of Pregnant Women for Diagnosis of Congenital Toxoplasmosis: A Systematic Review and Meta-Analysis

PLoS ONE ◽

10.1371/journal.pone.0149938 ◽

2016 ◽

Vol 11 (4) ◽

pp. e0149938 ◽

Cited By ~ 23

Author(s):

Christianne Terra de Oliveira Azevedo ◽

Pedro Emmanuel A. A. do Brasil ◽

Letícia Guida ◽

Maria Elizabeth Lopes Moreira

Keyword(s):

Systematic Review ◽

Polymerase Chain Reaction ◽

Amniotic Fluid ◽

Pregnant Women ◽

Meta Analysis ◽

Congenital Toxoplasmosis ◽

Polymerase Chain Reaction Analysis ◽

Chain Reaction ◽

Polymerase Chain

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Diagnostic Value of Multiplex Polymerase Chain Reaction in Detection of Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from Sepsis in Pediatrics

Recent Patents on Biotechnology ◽

10.2174/1872208315666210719104623 ◽

2021 ◽

Vol 15 ◽

Author(s):

Sara Galeb ◽

Maysaa El Sayed Zaki ◽

Raghdaa Shrief ◽

Rasha Hassan ◽

Mohamed Anies

Keyword(s):

Polymerase Chain Reaction ◽

Pseudomonas Aeruginosa ◽

Acinetobacter Baumannii ◽

Multiplex Pcr ◽

Stenotrophomonas Maltophilia ◽

Multiplex Polymerase Chain Reaction ◽

Diagnostic Value ◽

Blood Cultures ◽

Chain Reaction ◽

Polymerase Chain

Background: Proper identification of the causative organism in pediatric sepsis is crucial for early diagnosis and prevention of septic shock and organ failure. Objectives: The present study aimed to evaluate the multiplex Polymerase Chain Reaction (PCR) to detect Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures for these pathogens isolated from children, with hospital-acquired sepsis compared to the conventional biochemical reactions for identification of these organisms. Methods: This study was a cross-sectional study performed on 100 isolates from pediatric blood cultures, including Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. The study also included 100 isolates of Escherichia coli as a negative control. All isolates were identified by API 20NE and the multiplex PCR, with primers specific to the 3 tested bacteria. Results: Multiplex PCR was positive in 96% of isolates, and 4 isolates had negative results. False positive results were reported with three E. coli strains. Multiplex PCR identified all the isolates of Acinetobacter baumannii, 29 isolates of Pseudomonas aeruginosa, and 27 isolates of Stenotrophomonas maltophilia. Compared to the biochemical identification, the diagnostic value of the multiplex PCR revealed 96.04% sensitivity, 96.9% specificity, 97.00%, positive predictive value, 96.00% negative predictive value, and 96.50% accuracy. Conclusion: The present study highlights the diagnostic value of multiplex PCR to identify Acinetobacter baumannii, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia from positive blood cultures. Multiplex PCR was sensitive, specific, and accurate. The accuracy differs according to the organisms, with 100% accuracy for Acinetobacter baumannii.

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Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis

Ciência Rural ◽

10.1590/0103-8478cr20170871 ◽

2018 ◽

Vol 48 (6) ◽

Author(s):

Marcelo Marques da Silveira ◽

Stéfhano Luis Cândido ◽

Karin Rinaldi dos Santos ◽

Maerle Oliveira Maia ◽

Roberto Lopes de Souza ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Blood Culture ◽

Diagnostic Value ◽

Turnaround Time ◽

Diagnostic Techniques ◽

Pcr Analysis ◽

Chain Reaction ◽

Suspected Sepsis ◽

Fungal Dna ◽

Polymerase Chain

ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.

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