Polysorbate 20 non-ionic surfactant enhances retinal gene delivery efficiency of cationic niosomes after intravitreal and subretinal administration

2018 ◽  
Vol 550 (1-2) ◽  
pp. 388-397 ◽  
Author(s):  
Ilia Villate-Beitia ◽  
Idoia Gallego ◽  
Gema Martínez-Navarrete ◽  
Jon Zárate ◽  
Tania López-Méndez ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ionic Surfactant ◽  
Polysorbate 20 ◽  
Delivery Efficiency ◽  
Cationic Niosomes

scholarly journals Cationic Niosomes as Non-Viral Vehicles for Nucleic Acids: Challenges and Opportunities in Gene Delivery

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 50 ◽  
Author(s):  
Santiago Grijalvo ◽  
Gustavo Puras ◽  
Jon Zárate ◽  
Myriam Sainz-Ramos ◽  
Nuseibah A. L. Qtaish ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Self Assembly ◽  
Antisense Oligonucleotides ◽  
The Self ◽  
Ionic Surfactant ◽  
Colloidal Nanoparticles ◽  
Drug Molecules ◽  
Challenges And Opportunities ◽  
Good Number ◽  
Cationic Niosomes

Cationic niosomes have become important non-viral vehicles for transporting a good number of small drug molecules and macromolecules. Growing interest shown by these colloidal nanoparticles in therapy is determined by their structural similarities to liposomes. Cationic niosomes are usually obtained from the self-assembly of non-ionic surfactant molecules. This process can be governed not only by the nature of such surfactants but also by others factors like the presence of additives, formulation preparation and properties of the encapsulated hydrophobic or hydrophilic molecules. This review is aimed at providing recent information for using cationic niosomes for gene delivery purposes with particular emphasis on improving the transportation of antisense oligonucleotides (ASOs), small interference RNAs (siRNAs), aptamers and plasmids (pDNA).

scholarly journals FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-β-CyD

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Yiping Hu ◽  
Guping Tang ◽  
Jun Liu ◽  
Wenxiang Cheng ◽  
Ye Yue ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Human Cancer ◽  
Fibroblast Growth Factor Receptors ◽  
Fgf Receptor ◽  
Human Cancer Cells ◽  
High Gene ◽  
Targeting Ability ◽  
Delivery Efficiency

A novel vector with high gene delivery efficiency and special cell-targeting ability was developed using a good strategy that utilized low-molecular-weight polyethylenimine (PEI; molecular weight: 600 KDa [PEI600]) crosslinked toβ-cyclodextrin (β-CyD) via a facile synthetic route. Fibroblast growth factor receptors (FGFRs) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy. In this paper, CY11 peptides, which have been proven to combine especially with FGFRs on cell membranes were coupled to PEI-β-CyD usingN-succinimidyl-3-(2-pyridyldithio) propionate as a linker. The ratios of PEI600,β-CyD, and peptide were calculated based on proton integral values obtained from the1H-NMR spectra of the resulting products. Electron microscope observations showed that CY11-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer. Experiments on gene delivery efficiency in vitro showed that CY11-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the COS-7 and HepG2 cells, which positively expressed FGFR, whereas no such effect was observed in the PC-3 cells, which negatively expressed FGFR. Our current research indicated that the synthesized nonviral vector shows improved gene delivery efficiency and targeting specificity in FGFR-positive cells.

Improving Gene Delivery Efficiency of Bioreducible Poly(amidoamine)s via Grafting with Dendritic Poly(amidoamine)s

2010 ◽  
Vol 10 (4) ◽  
pp. 404-414 ◽  
Author(s):  
Ya-Nan Xue ◽  
Min Liu ◽  
Lin Peng ◽  
Shi-Wen Huang ◽  
Ren-Xi Zhuo
Keyword(s):  
Gene Delivery ◽  
Delivery Efficiency

Design and synthesis of a fluorescent amino poly(glycidyl methacrylate) for efficient gene delivery

2019 ◽  
Vol 7 (11) ◽  
pp. 1875-1881 ◽  
Author(s):  
Hongtao Chen ◽  
Shusen You ◽  
Qing Cai ◽  
Yang Zheng ◽  
Liqun Zhang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Atom Transfer Radical Polymerization ◽  
Radical Polymerization ◽  
Glycidyl Methacrylate ◽  
Dna Delivery ◽  
Atom Transfer ◽  
Design And Synthesis ◽  
Efficient Gene ◽  
Low Cytotoxicity ◽  
Delivery Efficiency

A fluorescent amino poly(glycidyl methacrylate) (PGOHMA) was synthesized by atom transfer radical polymerization (ATRP) and post-polymerization. PGOHMA has low cytotoxicity and high DNA delivery efficiency.

Plasmid DNA Size Does Affect Nonviral Gene Delivery Efficiency in Stem Cells

2012 ◽  
Vol 14 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Sofia Ribeiro ◽  
Juergen Mairhofer ◽  
Catarina Madeira ◽  
Maria Margarida Diogo ◽  
Cláudia Lobato da Silva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Nonviral Gene Delivery ◽  
Dna Size ◽  
Delivery Efficiency

Impact of Molecular Weight in Four-Branched Star Vectors with Narrow Molecular Weight Distribution on Gene Delivery Efficiency

2009 ◽  
Vol 20 (12) ◽  
pp. 2293-2299 ◽  
Author(s):  
Yasushi Nemoto ◽  
Alexey Borovkov ◽  
Yue-Min Zhou ◽  
Yoshiaki Takewa ◽  
Eisuke Tatsumi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Narrow Molecular Weight Distribution ◽  
Delivery Efficiency

Tunable PEGylation of branch-type PEI/DNA polyplexes with a compromise of low cytotoxicity and high transgene expression: in vitro and in vivo gene delivery

2017 ◽  
Vol 5 (24) ◽  
pp. 4732-4744 ◽  
Author(s):  
A. Venault ◽  
Y.-C. Huang ◽  
J. W. Lo ◽  
C.-J. Chou ◽  
A. Chinnathambi ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transgene Expression ◽  
In Vivo Gene Delivery ◽  
Low Cytotoxicity ◽  
Branch Type ◽  
Delivery Efficiency

Although PEGylated polyplexes for gene delivery are widespread, there is a need for an in-depth investigation of the role of the PEGylation degree on the delivery efficiency of the systems.

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