Background check: Profound differences in serum antibody isotypes among C57BL/6 mouse substrains discourage substrain interchanges in immunology experiments

2019 ◽  
Vol 216 ◽  
pp. 9-11 ◽  
Author(s):  
B.G. Jones ◽  
R.R. Penkert ◽  
S.L. Surman ◽  
R.E. Sealy ◽  
S. Pelletier ◽  
...  
Keyword(s):  
Serum Antibody ◽  
Background Check ◽  
Antibody Isotypes

scholarly journals Genetic parameters of colostrum and calf serum antibodies in Swedish dairy cattle

2021 ◽  
Author(s):  
Juan Miguel Cordero Solorzano ◽  
D. J. de Koning ◽  
Madeleine Tråvén ◽  
Therese de Haan ◽  
Mathilde Jouffroy ◽  
...  
Keyword(s):  
Genetic Parameters ◽  
Serum Antibody ◽  
Genetic Correlations ◽  
Calf Serum ◽  
Passive Immunity ◽  
Probability Of Survival ◽  
Antibody Isotypes ◽  
Swedish Dairy ◽  
Indicator Traits ◽  
Antibody Levels

Background: Colostrum with sufficient IgG content is essential for the newborn calf, as it provides passive immunity which substantially affects the probability of survival during rearing. Failure of passive transfer occurs when a calf does not absorb enough antibodies from the colostrum as indicated by less than 10 g/L of IgG in calf serum. Besides delayed access to colostrum, this can be due to low IgG production of the mother or poor IgG absorption by the calf. The aim of this study was to estimate the genetic background of antibody levels and indicator traits for antibodies in colostrum and calf serum, and their correlation with milk production and health. Results: Colostrum data were available from 1340 cows with at least one calving and calf serum data were available from 886 calves from these cows. Antibody concentrations were estimated using refractometry (digital Brix refractometer for colostrum and optical refractometer for serum) as indicator traits and established using ELISAs to determine total IgG and natural antibodies [NAb] of various antibody isotypes in colostrum and serum. Colostrum traits had heritabilities ranging from 0.16 to 0.31 with repeatabilities from 0.21 to 0.55. Brix had positive genetic correlations with all colostrum antibody traits including total IgG (0.68). Calf serum antibody concentrations had heritabilities ranging from 0.25 to 0.59, with a significant maternal effect accounting for 17 to 27% of the variance. When calves later in life produced their first lactation, lactation-average somatic cell score was found to be negatively correlated with NAb in calf serum. Conclusions: Our results suggest that antibody levels in colostrum and calf serum can be increased by means of selection.

Linkage between H. pylori Infection and TNF-α polymorphism in The Pregnant Women

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ghaidaa Raheem Lateef ◽  
Azhar Omaran Al-Thahab
Keyword(s):  
Pregnant Women ◽  
Serum Antibody ◽  
Rapid Test ◽  
Negative Group ◽  
Positive Group ◽  
Gynecology And Obstetrics ◽  
Tnf Α ◽  
Significant Difference ◽  
Pcr Products ◽  
H Pylori

A study was performed on 100 pregnant women in the outpatient department of gynecology and obstetrics of Maternity and Children Hospital in Al-Diwaniya City during the period between (March to September 2016). One hundred blood samples (50 for patients and 50 for control) were collected under the supervision of the treating gynecologist. The detection of Helicobacter. pylori was done by the use of the serum antibody Rapid test. The results showed that 50 (100%) were positive and 50 (100%) were negative for H. pylori in above method.All blood of patients and control samples were used for the extraction of genomic DNA,where the 107 bp PCR product size. Genotyping of the TNF-α-308 SNP (G/A)was performed by restriction fragment length polymorphism PCR (RFLP-PCR). PCR products were digested with restr NcoI iction enzyme. Individuals with the TNF-α-308(GG) homozygote produced digested DNA bands at 80,and 20 bp bp. A heterozygous genotype ofTNF-α-308 (GA)produced 107 bp,80 bp,and 20 bp bands. Individuals with the TNF-α-308 (AA) homozygote genotype had no amplicon digested and generated only one band of 107 bp. There was a significant difference in the frequency of the TNF-α-308(GG)genotype between H. pylori positive group and H. pylori negative group(72%,78% respectively). Also for GA genotype,there was a significant difference between H. pylori positive group and H. pylori negative group(24%,18% respectively). Concerning the frequency of the TNF-α-308 (AA)genotype between H. pylori positive group and H. pylori negative group,there was no significant difference between the two groups.

Serum Antibody Against NY-ESO-1 and XAGE1 Predicts Clinical Responses to Anti-PD-1 Therapy in Non-Small-Cell Lung Cancer

2018 ◽  
Author(s):  
Yoshihiro Ohue ◽  
Koji Kurose ◽  
Takahiro Karasaki ◽  
Midori Isobe ◽  
Takaaki Yamaoka ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Serum Antibody ◽  
Small Cell ◽  
Small Cell Lung ◽  
Clinical Responses

Antibody response of carp, Cyprinus carpio to the cestode, Bothriocephalus acheilognathi

Parasitology ◽  
1999 ◽  
Vol 118 (6) ◽  
pp. 635-639 ◽  
Author(s):  
P. NIE ◽  
D. HOOLE
Keyword(s):  
Antibody Response ◽  
Cyprinus Carpio ◽  
Serum Antibody ◽  
Infected Fish ◽  
Immune Protection ◽  
Carp Cyprinus Carpio ◽  
Bothriocephalus Acheilognathi ◽  
Protection Against Infection ◽  
Antibody Levels ◽  
Antibody Secreting Cells

The humoral antibody response and the number of pronephric antibody-secreting cells were examined in naturally Bothriocephalus acheilognathi-infected carp. Cyprinus carpio, and in those injected intraperitoneally with an extract of the cestode. In the extract-injected fish, specific antibody was detected 3 weeks after a second injection given 2 weeks after the primary injection, and antibody levels persisted for more than 200 days. A third injection also enhanced the antibody level in the extract-injected carp. The numbers of antibody-secreting cells were significantly higher in carp injected 3 times with the extract than in the control. In naturally-infected fish, the serum antibody levels and the number of pronephric antibody-secreting cells were higher in infected fish than in uninfected individuals although this difference was not statistically significant. The relevance of these results to immune protection against infection is discussed.

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