Proteomic analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation

2014 ◽  
Vol 59 ◽  
pp. 299-308 ◽  
Author(s):  
Mengjun Huang ◽  
Yang Fang ◽  
Yao Xiao ◽  
Jiaolong Sun ◽  
Yanling Jin ◽  
...  
2021 ◽  
pp. 29-33
Author(s):  
А.В. Митюшкин ◽  
А.А. Журавлев ◽  
А.В. Митюшкин ◽  
А.С. Гайзатулин ◽  
С.С. Салюков ◽  
...  

В 2018-2020 годах проведено сравнительное изучение высококрахмалистых сортов картофеля по интенсивности накопления крахмала и размеру крахмальных зерен. Полевые и лабораторные исследования выполнены на экспериментальных базах «Пышлицы» и «Коренево» ФИЦ картофеля имени А.Г. Лорха (Московская область). В качестве исходного материала в опытах использовали шесть сортов картофеля собственной селекции среднераннего (Артур, Садон) и среднеспелого (Авангард, Каскад, Накра, Синеглазка 2016) сроков созревания. Объект исследования – нативный крахмал, полученный по ГОСТ 7699-78 из клубней для определения размера крахмальных зерен безмикроскопным методом. Установлена вариабельность крахмалистости изученных сортов с учетом генотипических особенностей, сроков созревания и условий выращивания. Выявлены наиболее стабильные по уровню крахмалонакопления в клубнях сорта Артур, Каскад, Накра и Синеглазка 2016. Различия в крахмалистости между средними и мелкими клубнями одного и того же сорта более существенны в сравнении с различиями между средними и мелкими клубнями. В летней пробе по уровню крахмалистости клубней выделились сорта Накра (20,0%) и Каскад (19,2%) в сравнении с сортом-стандартом Зарево (19,6%), а также сорта Артур и Синеглазка 2016 с крахмалистостью 18,6% и 18,5% соответственно. При анализе высококрахмалистых сортов показано, что в составе крахмала наибольшее количество крахмальных зерен более 57 мкм содержится в клубнях сортов Накра (60,8%), Синеглазка 2016 (60,5%), Каскад (55,7%) и Артур (56,3%). In 2018-2020, a comparative study of high-starch potato varieties was carried out according to the intensity of starch accumulation and the size of starch grains. Field and laboratory studies were carried out at the experimental bases «Pyshlitsy» and «Korenevo» FPRC of named after A.G. Lorkh (Moscow region). Six varieties of potatoes of their own selection of medium-early (Artur, Sadon) and medium-ripe (Avangard, Kaskad, Nakra, Sineglazka 2016) maturation dates were used as the starting material in the experiments. The object of research is native starch obtained according to GOST 7699-78 from tubers for determining the size of starch grains by a non microscopic method. The variability of the starch content of the studied varieties was established, taking into account genotypic features, maturation periods and growing conditions. The most stable in terms of starch accumulation in tubers of the varieties Artur, Kaskad, Nakra and Sineglazka 2016 were identified. Differences in starchiness between medium and small tubers the same varieties are more significant in comparison with the differences between medium and small tubers. In the summer sample, according to the level of starchiness of tubers, the varieties Nakra (20.0%) and Kaskad (19.2%) were distinguished in comparison with the standard variety Zarevo (19.6%), as well as the varieties Artur and Sineglazka 2016 with a starchiness of 18.6 and 18.5%, respectively. When analyzing highly starchy varieties, it was shown that the largest number of starch grains in the starch composition is more than 57 microns contained in the tubers of the varieties Nakra (60.8%), Sineglazka 2016 (60.5%), Kaskad (55.7%) and Artur (56.3%).


Agronomy ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 297 ◽  
Author(s):  
Anon Janket ◽  
Nimitr Vorasoot ◽  
Banyong Toomsan ◽  
Wanwipa Kaewpradit ◽  
Poramate Banterng ◽  
...  

The information on genotypic responses to the seasonal variation in the starch content, starch yield, and starch quality of cassava is limited. The objective of this research was to investigate the seasonal variation of starch characteristics of three cassava genotypes grown under irrigation. The experiment was conducted at four planting dates (20 April, 30 June, 5 October, and 15 December 2015). Three cassava genotypes (CMR38-125-77, Kasetsart 50, and Rayong 11) were evaluated in a randomized complete block design with four replications and the plants were harvested at 12 months. The planting date contributed the largest portion of the total variation in the starch content, starch yield, and starch granule size. The amylose content variability was heavily influenced by genotype. Cassava planted on 5 October or 15 December had greater starch content, starch yield, and starch granule in most genotypes. This was likely due to a higher temperature and solar radiation during the 3–9 months post-planting. CMR38-125-77 showed a consistently high starch content, starch yield, and high amylose content for most planting dates except for the starch yield on 20 April, of which Rayong 11 was the best. These findings will be useful for choosing suitable cassava genotypes for different growing seasons and for facilitating breeding efforts for high starch-yielding and high-quality cassava starch in the future.


BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Dingding Cao ◽  
Rebecca Njeri Damaris ◽  
Yue Zhang ◽  
Meihui Liu ◽  
Ming Li ◽  
...  

Abstract Background Rhizome is the storage underground stem of lotus (Nelumbo nucifera), which is enlarged before winter season and could be used for asexual propagation. In addition, the enlarged rhizome is a nutritional vegetable with abundant starch, proteins, and vitamins. Enlargement of lotus rhizome is not only significance for itself to survive from the cold winter, but also important for its economic value. Results To explore the mechanism underlying its enlargement, integrative analyses of morphology, physiology and proteomics were conducted on the rhizome at stolon, middle, and enlarged stages. Morphological observation and physiological analyses showed that rhizomes were gradually enlarged during this process, in which the starch accumulation was also initiated. Quantitative proteomic analysis on the rhizomes at these three stages identified 302 stage-specific proteins (SSPs) and 172 differently expressed proteins (DEPs), based on which GO and KEGG enrichment analyses were conducted. The results indicated that light and auxin signal might be transduced through secondary messenger Ca2+, and play important roles in lotus rhizome enlargement. Conclusion These results will provide new insights into understanding the mechanism of lotus rhizome enlargement. Meanwhile, some candidate genes might be useful for further studies on this process, as well as breeding of rhizome lotus.


1991 ◽  
Vol 138 (2) ◽  
pp. 186-190 ◽  
Author(s):  
A.A. Schaffer ◽  
H. Nerson ◽  
E. Zamski

2015 ◽  
Vol 18 (1) ◽  
pp. 24-36 ◽  
Author(s):  
Celia G. Jerez ◽  
José R. Malapascua ◽  
Magda Sergejevová ◽  
Félix L. Figueroa ◽  
Jiří Masojídek

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