scholarly journals Proteomic analysis showing the signaling pathways involved in the rhizome enlargement process in Nelumbo nucifera

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Dingding Cao ◽  
Rebecca Njeri Damaris ◽  
Yue Zhang ◽  
Meihui Liu ◽  
Ming Li ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Economic Value ◽  
Winter Season ◽  
Nelumbo Nucifera ◽  
Morphological Observation ◽  
Starch Accumulation ◽  
Asexual Propagation ◽  
Secondary Messenger ◽  
Specific Proteins ◽  
Three Stages

Abstract Background Rhizome is the storage underground stem of lotus (Nelumbo nucifera), which is enlarged before winter season and could be used for asexual propagation. In addition, the enlarged rhizome is a nutritional vegetable with abundant starch, proteins, and vitamins. Enlargement of lotus rhizome is not only significance for itself to survive from the cold winter, but also important for its economic value. Results To explore the mechanism underlying its enlargement, integrative analyses of morphology, physiology and proteomics were conducted on the rhizome at stolon, middle, and enlarged stages. Morphological observation and physiological analyses showed that rhizomes were gradually enlarged during this process, in which the starch accumulation was also initiated. Quantitative proteomic analysis on the rhizomes at these three stages identified 302 stage-specific proteins (SSPs) and 172 differently expressed proteins (DEPs), based on which GO and KEGG enrichment analyses were conducted. The results indicated that light and auxin signal might be transduced through secondary messenger Ca2+, and play important roles in lotus rhizome enlargement. Conclusion These results will provide new insights into understanding the mechanism of lotus rhizome enlargement. Meanwhile, some candidate genes might be useful for further studies on this process, as well as breeding of rhizome lotus.

THU0021 Cartilage zone specific proteins: A quantitative proteomic analysis

2013 ◽  
Vol 71 (Suppl 3) ◽  
pp. 160.3-160
Author(s):  
P. Fernández-Puente ◽  
L. Lourido ◽  
V. Calamia ◽  
J. Mateos ◽  
C. Ruiz-Romero ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Quantitative Proteomic Analysis ◽  
Specific Proteins

scholarly journals Key Regulatory Pathways, MicroRNAs, and Target Genes Participate in Adventitious Root Formation of Acer Rubrum L

2021 ◽  
Author(s):  
Wenpeng Zhu ◽  
Manyu Zhang ◽  
Jianyi Li ◽  
Hewen Zhao ◽  
Kezhong Zhang ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Seedling Survival ◽  
Expression Patterns ◽  
Economic Value ◽  
Acer Rubrum ◽  
Pcr Analysis ◽  
Asexual Propagation ◽  
Regulatory Pathways

Abstract BackgroundAcer rubrum L. is a colorful ornamental tree with great economic value. Because this tree is difficult to root under natural conditions and the seedling survival rate is low, vegetative propagation methods are often used. Because the formation of adventitious roots (ARs) is essential for the survival of asexual propagation of A. rubrum, it is necessary to investigate the molecular regulatory mechanisms in the formation of ARs of A. ruburm. To address this knowledge gap, we sequenced the transcriptome and sRNA of the A. rubrum variety ‘Autumn Fantasy’ using high-throughput sequencing and explored changes in gene and microRNA (miRNA) expression in response to exogenous auxin treatment. ResultsWe identified 82,468 differentially expressed genes between the treated and untreated ARs, as well as 48 known and 95 novel miRNAs. We also identified 172 target genes of the known miRNAs using degradome sequencing. Two regulatory pathways (ubiquitin mediated proteolysis and plant hormone signal transduction), Ar-miR160a and the target gene ArARF10 were shown to be involved in the auxin response. We further investigated the expression patterns and regulatory roles of ArARF10 through subcellular localization, transcriptional activation, plant transformation, qRT-PCR analysis, and GUS staining. ConclusionsDifferential expression patterns indicated the Ar-miR160a-ArARF10 interaction might play a significant role in the regulation of AR formation in A. rubrum. Our study provided new insights into mechanisms underlying the regulation of AR formation in A. rubrum.

scholarly journals Seasonal status of white spot syndrome virus in broodstocks, nauplii and postlarvae of black tiger shrimp (Penaeus monodon) in Bangladesh

1970 ◽  
Vol 1 (3) ◽  
pp. 56-61 ◽  
Author(s):  
MM Iqbal ◽  
MA Kabir ◽  
CB Alan ◽  
MAA Mamun ◽  
MM Hossain
Keyword(s):  
White Spot Syndrome Virus ◽  
Monsoon Season ◽  
Penaeus Monodon ◽  
Winter Season ◽  
White Spot ◽  
Water Current ◽  
Fluctuation Pattern ◽  
Three Stages ◽  
Polymerase Chain ◽  
White Spot Syndrome

Seasonal variation of white spot syndrome virus (WSSV) prevalence in broodstocks shrimp, nauplii and postlarvae were investigated from 2004 to 2006 in Bangladesh by polymerase chain reaction (PCR) assay. There was consistent pattern of fluctuation of WSSV prevalence in broodstocks, nauplii and postlarvae. WSSV fluctuation pattern in three stages were more or less similar for each year. Average lowest prevalence in broodstock was 0% in September and highest was 90% in May-June during the monsoon season in Bangladesh. The WSSV fluctuation patterns for nauplius correlated with broodstock patterns with average lowest prevalence of 0% in September compared to the highest of 40% in July. Similarly, average lowest prevalence of WSSV in postlarvae was 0% in December and highest was 14% in July. It was evident from this study that WSSV prevalence in broodstock, nauplius and postlarvae remained high during the monsoon season and low during the winter season when the water current, salinity and tidal flashing remained more or less stable in the Bay of Bengal in Bangladesh. Key words: Bangladesh; broodstocks; nauplii; postlarvae; seasonal; shrimp; WSSV. DOI: http://dx.doi.org/10.3329/ijns.v1i3.8822 International Journal of Natural Sciences (2011), 1(3):56-61

Proteomic analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation

2014 ◽  
Vol 59 ◽  
pp. 299-308 ◽  
Author(s):  
Mengjun Huang ◽  
Yang Fang ◽  
Yao Xiao ◽  
Jiaolong Sun ◽  
Yanling Jin ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Nutrient Starvation ◽  
Starch Accumulation ◽  
High Starch

scholarly journals Key Regulatory Pathways, microRNAs, and Target Genes Participate in Adventitious Root Formation of Acer Rubrum L.

2021 ◽  
Author(s):  
Wenpeng Zhu ◽  
Manyu Zhang ◽  
Jianyi Li ◽  
Hewen Zhao ◽  
Kezhong Zhang ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Seedling Survival ◽  
Expression Patterns ◽  
Economic Value ◽  
Acer Rubrum ◽  
Pcr Analysis ◽  
Asexual Propagation ◽  
Regulatory Pathways

Abstract BackgroundAcer rubrum L. is a colorful ornamental tree with great economic value. Because this tree is difficult to root under natural conditions and the seedling survival rate is low, vegetative propagation methods are often used. Because the formation of adventitious roots (ARs) is essential for the survival of asexual propagation of A. rubrum, it is necessary to investigate the molecular regulatory mechanisms in the formation of ARs of A. ruburm. To address this knowledge gap, we sequenced the transcriptome and sRNA of the A. rubrum variety ‘Autumn Fantasy’ using high-throughput sequencing and explored changes in gene and microRNA (miRNA) expression in response to exogenous auxin treatment. ResultsWe identified 82,468 differentially expressed genes between the treated and untreated ARs, as well as 48 known and 95 novel miRNAs. We also identified 172 target genes of the known miRNAs using degradome sequencing. Two regulatory pathways (ubiquitin mediated proteolysis and plant hormone signal transduction), Ar-miR160a and the target gene ArARF10 were shown to be involved in the auxin response. We further investigated the expression patterns and regulatory roles of ArARF10 through subcellular localization, transcriptional activation, plant transformation, qRT-PCR analysis, and GUS staining. ConclusionsDifferential expression patterns indicated the Ar-miR160a-ArARF10 interaction might play a significant role in the regulation of AR formation in A. rubrum. Our study provided new insights into mechanisms underlying the regulation of AR formation in A. rubrum.

scholarly journals Analisis Pengembangan Budidaya Rumput Laut (Euchema cottoni) di Perairan Kosiwo Kabupaten Yapen

2021 ◽  
Vol 2 (2) ◽  
pp. 66-70
Author(s):  
Richard Yohanes Mambai ◽  
Suryawati Salam ◽  
Erni Indrawati
Keyword(s):  
Production Management ◽  
Swot Analysis ◽  
Economic Value ◽  
External Environment ◽  
Seaweed Cultivation ◽  
Quality Factors ◽  
Seaweed Farming ◽  
Three Stages ◽  
Derivative Products ◽  
Eucheuma Cottonii

Rumput laut merupakan salah satu komoditas budidaya laut yang memiliki nilai ekonomis tinggi. Upaya mengembangkan budidaya rumput laut diperlukan untuk meningkatkan produksi serta meningkatkan pendapatan masyarakat pesisir. Keberhasilan budidaya rumput laut sangat dipengaruhi oleh faktor kualitas perairan yang mendukung dan sesuai untuk pertumbuhannya. Penelitian ini bertujuan menganalisis lingkungan internal dan eksternal yang mempengaruhi budidaya  dan  mengkaji strategi yang tepat dalam usaha pengembangan budidaya rumput laut (Eucheuma cottonii) di perairan distrik Kosiwo Kabupaten Kepulauan Yapen. Metode yang digunakan adalah analisis data eksternal dan internal yang di analisis SWOT melalui tiga tahap analisis yaitu tahap pengumpulan data, tahap analisis dan tahap pengambilan keputusan. Hasil penelitian menunjukkan lingkungan internal yang mempengaruhi perkembangan budidaya rumput laut di distrik Kosiwo adalah produksi; manajeman dan pendanaan; sumber daya manusia; investasi dan lokasi budidaya. Lingkungan eksternal yang mempengaruhi perkembangan usaha budidaya rumput laut di Distrik Kosiwo adalah ekonomi, sosial budaya dan lingkungan; pasar; pesaing; IPTEK serta iklim dan cuaca. Strategi yang dapat digunakan mengadakan pelatihan tentang budidaya, penanganan penyakit dan pengolahan produk turunan untuk meningkatkan keterampilan pembudidaya sehingga mampu menghasilkan produk dan memanfaatkan lahan budidaya  untuk menghasilkan rumput laut dalam jumlah besar agar mampu memperluas jaringan pemasaran Seaweed is one of the marine cultivation commodities which has high economic value. Efforts to develop seaweed cultivation are needed to increase the production and the income of coastal communities. The success of seaweed cultivation is strongly influenced by the water quality factors that support and are suitable for its growth. This study aims to analyze the internal and external environment that affect cultivation and to assess appropriate strategies in the development of seaweed (Eucheuma cottonii) cultivation in the coastal area of Kosiwo district, Kepulauan Yapen Regency. The method used is external and internal data analysis, which is SWOT analysis through three stages of analysis, namely the data collection stage, the analysis stage and the decision-making stage. The results showed that the internal environment that affects the development of seaweed cultivation in Kosiwo district is production; management and funding; human Resources; investment and cultivation locations. The external environment that affects the development of seaweed farming in Kosiwo District is the economy, socio-culture and environment; market; competitor; Science and technology and climate and weather. Strategies that can be used are to hold a training on cultivation, disease and processing management of derivative products to improve the skills of cultivators so that they are able to produce products and utilize cultivated land to produce seaweed in large quantities in order to be able to expand the marketing network.

scholarly journals Phytochemical Screening, Antioxidant and Sperm Viability of Nelumbo nucifera Petal Extracts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1375
Author(s):  
Jiraporn Laoung-on ◽  
Churdsak Jaikang ◽  
Kanokporn Saenphet ◽  
Paiwan Sudwan
Keyword(s):  
Antioxidant Activity ◽  
Agricultural Waste ◽  
Economic Value ◽  
Nelumbo Nucifera ◽  
Sperm Viability ◽  
Phytochemical Content ◽  
Sacred Lotus ◽  
Phenolics Content ◽  
Vitro Model

Sacred lotus (Nelumbo nucifera Gaertn.; N. nucifera) is a common ingredient in traditional medicine and Thai recipes. Its petal is an agricultural waste from stamen production. There are limitations in the used and pharmacological data of the petals resulting in more petals waste. The aims of this study were to investigate the phytochemical contents, antioxidant activity, and potential effects on sperm viability of aqueous (NAE) and ethanolic extracts (NEE) of both red and white N. nucifera petals. The white NAE had the highest total phenolics content, total tannins content and maximal antioxidant activity. The white NEE had the highest concentration of total flavonoids. Quercetin was a major flavonoid and was found in the aqueous extracts. Both red and white of NAE in the range of 0.22 to 1.76 mg/mL increased sperm viability. The white NAE was prominent in phytochemical content, antioxidant activity, and both red and white NAE effectively increased rat sperm viability in the in vitro model. The white NAE enhanced sperm viability by decreasing oxidative stress. It might be suggested that the N. nucifera petals have benefits for sperm viability health promotion and may increase the economic value of agricultural waste.

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