Evaluation of highly starchy potato varieties by the intensity of starch accumulation and the size of starch grains

2021 ◽  
pp. 29-33
Author(s):  
А.В. Митюшкин ◽  
А.А. Журавлев ◽  
А.В. Митюшкин ◽  
А.С. Гайзатулин ◽  
С.С. Салюков ◽  
...  
Keyword(s):  
Starch Content ◽  
Starch Accumulation ◽  
Starch Grains ◽  
Potato Varieties ◽  
Summer Sample ◽  
High Starch ◽  
Starch Composition ◽  
Growing Conditions ◽  
Starch Potato ◽  
Selection Of

В 2018-2020 годах проведено сравнительное изучение высококрахмалистых сортов картофеля по интенсивности накопления крахмала и размеру крахмальных зерен. Полевые и лабораторные исследования выполнены на экспериментальных базах «Пышлицы» и «Коренево» ФИЦ картофеля имени А.Г. Лорха (Московская область). В качестве исходного материала в опытах использовали шесть сортов картофеля собственной селекции среднераннего (Артур, Садон) и среднеспелого (Авангард, Каскад, Накра, Синеглазка 2016) сроков созревания. Объект исследования – нативный крахмал, полученный по ГОСТ 7699-78 из клубней для определения размера крахмальных зерен безмикроскопным методом. Установлена вариабельность крахмалистости изученных сортов с учетом генотипических особенностей, сроков созревания и условий выращивания. Выявлены наиболее стабильные по уровню крахмалонакопления в клубнях сорта Артур, Каскад, Накра и Синеглазка 2016. Различия в крахмалистости между средними и мелкими клубнями одного и того же сорта более существенны в сравнении с различиями между средними и мелкими клубнями. В летней пробе по уровню крахмалистости клубней выделились сорта Накра (20,0%) и Каскад (19,2%) в сравнении с сортом-стандартом Зарево (19,6%), а также сорта Артур и Синеглазка 2016 с крахмалистостью 18,6% и 18,5% соответственно. При анализе высококрахмалистых сортов показано, что в составе крахмала наибольшее количество крахмальных зерен более 57 мкм содержится в клубнях сортов Накра (60,8%), Синеглазка 2016 (60,5%), Каскад (55,7%) и Артур (56,3%). In 2018-2020, a comparative study of high-starch potato varieties was carried out according to the intensity of starch accumulation and the size of starch grains. Field and laboratory studies were carried out at the experimental bases «Pyshlitsy» and «Korenevo» FPRC of named after A.G. Lorkh (Moscow region). Six varieties of potatoes of their own selection of medium-early (Artur, Sadon) and medium-ripe (Avangard, Kaskad, Nakra, Sineglazka 2016) maturation dates were used as the starting material in the experiments. The object of research is native starch obtained according to GOST 7699-78 from tubers for determining the size of starch grains by a non microscopic method. The variability of the starch content of the studied varieties was established, taking into account genotypic features, maturation periods and growing conditions. The most stable in terms of starch accumulation in tubers of the varieties Artur, Kaskad, Nakra and Sineglazka 2016 were identified. Differences in starchiness between medium and small tubers the same varieties are more significant in comparison with the differences between medium and small tubers. In the summer sample, according to the level of starchiness of tubers, the varieties Nakra (20.0%) and Kaskad (19.2%) were distinguished in comparison with the standard variety Zarevo (19.6%), as well as the varieties Artur and Sineglazka 2016 with a starchiness of 18.6 and 18.5%, respectively. When analyzing highly starchy varieties, it was shown that the largest number of starch grains in the starch composition is more than 57 microns contained in the tubers of the varieties Nakra (60.8%), Sineglazka 2016 (60.5%), Kaskad (55.7%) and Artur (56.3%).

SELECTION OF MEDIUM EARLY AND MID-SEASON VARIETIES OF POTATOES FOR THE STEPPE ZONE OF SOUTHERN URALS

2016 ◽  
Vol 1 (4) ◽  
pp. 18-21
Author(s):  
Аминова ◽  
Evgeniya Aminova ◽  
Мушинский ◽  
Aleksandr Mushinskiy ◽  
Герасимова ◽  
...  
Keyword(s):  
Dry Matter ◽  
Starch Content ◽  
Steppe Zone ◽  
Southern Urals ◽  
Matter Content ◽  
Dry Matter Content ◽  
Potato Tubers ◽  
Potato Varieties ◽  
Selection Of

The purpose of research is to identify the most adaptive and productive varieties of potatoes for the steppe zone of Southern Urals. The experience was laid by one-factor circuit in 3-fold repetition. The studies were conducted for middle- and middle-early varieties of potatoes domestic and foreign selection. Determined yield, marketability, starch and dry content in potato tubers. At 4 years of data were allocated potato varieties – Artemis (57.8 t/ha), Aerrow (52.0 t/ha) and Romano (49.5 t/ha). Distinguished highest yield of marketable products varieties: Artemis – 97.9%, Tarasov – 97.7%, and Aerrow Romano – 97.4%. The starch content in tubers was measurable by 10.2% (Ed Scarlett) to 16.0% (Kovalenko Memory), and the dry matter content in tubers ranged from 15.6% (Ed Scarlett) to 23.8% (Memory Kovalenko).

scholarly journals Результаты испытания новых перспективных сортов картофеля

2019 ◽  
Author(s):  
Kh.Kh. Apshev ◽  
N.A. Timoshina ◽  
E.V. Knyazeva ◽  
L.S. Fedotova
Keyword(s):  
Vitamin C ◽  
Reducing Sugars ◽  
Starch Content ◽  
Nitrate Content ◽  
High Starch Content ◽  
Early Ripening ◽  
New Varieties ◽  
High Starch ◽  
Selection Of ◽  
Red Pulp

В работе представлена характеристика восьми новых сортов картофеля отечественной селекции ранней и среднеранней групп спелости: Кладезь, Корчма, Кралек, Купец, Патриот, Призер, Третьяковка, Эликсир. Продуктивность новых сортов выше стандартов на 1,5–14,7 т/га, они характеризуются высокой крахмалистостью и содержанием витамина С, отличным вкусом, низким содержанием нитратов и средним – редуцирующих сахаров. Особого внимания заслуживают сорта Кралек (розовая мякоть) и Эликсир (красная мякоть) для диетического питания с повышенным содержанием антоцианов (антиоксидантов) и отличным вкусом.The work presents the characteristics of eight new varieties of potato of russian selection of early and early ripening groups: Kladez, Korchma, Kralek, Kupets, Patriot, Prizier, Tretyakovka, Elixir. Productivity of new varieties is 1.5–14.7 t/ha higher than standards. New varieties characterize high starch content, vitamin C content, excellent taste, low nitrate content and medium – reducing sugars. Special attention should be paid to the varieties Kralek (pink pulp) and Elixir (red pulp). These varieties are intended for dietary nutrition and contains anthocyanins (antioxidants). Also they differ excellent taste.

scholarly journals The collection of potato varieties as a reserve of source material for breeding for high yield, earliness, and resistance to diseases and pests

2020 ◽  
Vol 181 (2) ◽  
pp. 50-56
Author(s):  
L. I. Kostina ◽  
O. S. Kosareva ◽  
E. V. Truskinov ◽  
T. V. Kirpicheva
Keyword(s):  
Late Blight ◽  
Starch Content ◽  
Cyst Nematode ◽  
Source Material ◽  
High Yield ◽  
Colorado Beetle ◽  
Potato Varieties ◽  
High Starch Content ◽  
Leptinotarsa Decemlineata Say ◽  
High Starch

Background. Information is provided about the results obtained by screening the VIR collection of improved potato varieties for main commercial traits: earliness, high yield, high starch content, and resistance to late blight (Phytophthora infestans (Mont.) de Bary), viruses, and pests, such as cyst nematode (Globodera rostochiensis Woll. (Ro1)), and Colorado beetle (Leptinotarsa decemlineata Say).Materials and methods. Potato accessions representing domestic and foreign varieties from the VIR collection served as the material for this research. The screening was performed according to the techniques developed at the Potato Genetic Resources Department of VIR.Results and conclusion. Potato varieties with valuable commercial traits were identified as breeding sources promising for earliness: ‘Bashkirskiy’ (k-25338), ‘Charoit’ (k-25221), ‘Colleen’ (k-25224), ‘Lagun’ (k-25294), etc.; for high yield: ‘Ametist’ (k-25336), ‘Banba’ (k-25222), ‘Bastion’ (k-25198), ‘Nur-Alem’ (k-25253), ‘Senim’ (k-25306), etc.; for high starch content: ‘Charaўnik’ (k-25139), ‘Darnitsa’ (k-25179), ‘Lad’ (k-25180), ‘Maksimum’ (k-25136), etc.; for resistance to late blight: local variety (Georgia) (k-25298, k-25326), ‘Sunkar’ (k-25258), ‘Udovitskiy’ (k-25260), ‘Vektor’ (k-25200), ‘Zvezdochka’ (k-25209); for resistance to viruses: ‘Azart’ (k-25196), Gala (k-25270), ‘Nadezhda’ (k-25213), Utro (k-25219), ‘Volat’ (k-25263), etc. Varieties combining resistance to gold potato cyst nematode with other valuable commercial traits were selected. Varieties with relative resistance to Colorado potato beetle were isolated under favorable conditions for the pest’s development. As a result of the research, new source material was identified, with a potential to improve major commercial traits of potato: earliness, high yield, high starch content, resistance to late blight, viruses, and pests (cyst nematode and Colorado beetle).

Starch concentration of native Andean potato varieties (Solanum spp.)

2020 ◽  
Vol 2 (1) ◽  
pp. 15-25
Author(s):  
Severo Ignacio-Cardenas ◽  
◽  
Eugenio Perez Trujillo ◽  
Fernando Jeremias Gonzales Pariona
Keyword(s):  
Potato Starch ◽  
Starch Content ◽  
Human Consumption ◽  
Starch Concentration ◽  
Potato Varieties ◽  
Analysis Techniques ◽  
Andean Potato ◽  
And Cluster Analysis ◽  
Starch Potato ◽  
Solanum Spp

The study consisted of determination the native starch content in the tubers of the seven landraces potato, developed according to the quantitative approach framework. The samplings of tubers were developed by transect technique, in three production places from Quío town. The starch concentration of the tubers was analyzed in the laboratory of the Agricultural Sciences Faculty at UNHEVAL by the decanting method. The data were analyzed using the descriptive statistics, contingency and cluster analysis techniques. Starch content variation in landraces potato were from 12.44% in Tarmeña variety to 20.19% in Ishcupuru variety. The results showed that is necessary to be directed towards other bigger studies that approaches alternatives for to promote the human consumption of native potatoes of high nutritional quality food, its use in the food industry; in addition, to developed other research about the genetic improvement of native potatoes for to select varieties that synthesize larger amounts of starch and other industrial value substances. Keywords: Native Potato Starch, Potato Varieties, Transect

scholarly journals Seasonal Variation in Starch Accumulation and Starch Granule Size in Cassava Genotypes in a Tropical Savanna Climate

Agronomy ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 297 ◽  
Author(s):  
Anon Janket ◽  
Nimitr Vorasoot ◽  
Banyong Toomsan ◽  
Wanwipa Kaewpradit ◽  
Poramate Banterng ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Starch Granule ◽  
Amylose Content ◽  
Starch Content ◽  
Block Design ◽  
Granule Size ◽  
Starch Accumulation ◽  
Planting Dates ◽  
High Starch ◽  
Starch Yield

The information on genotypic responses to the seasonal variation in the starch content, starch yield, and starch quality of cassava is limited. The objective of this research was to investigate the seasonal variation of starch characteristics of three cassava genotypes grown under irrigation. The experiment was conducted at four planting dates (20 April, 30 June, 5 October, and 15 December 2015). Three cassava genotypes (CMR38-125-77, Kasetsart 50, and Rayong 11) were evaluated in a randomized complete block design with four replications and the plants were harvested at 12 months. The planting date contributed the largest portion of the total variation in the starch content, starch yield, and starch granule size. The amylose content variability was heavily influenced by genotype. Cassava planted on 5 October or 15 December had greater starch content, starch yield, and starch granule in most genotypes. This was likely due to a higher temperature and solar radiation during the 3–9 months post-planting. CMR38-125-77 showed a consistently high starch content, starch yield, and high amylose content for most planting dates except for the starch yield on 20 April, of which Rayong 11 was the best. These findings will be useful for choosing suitable cassava genotypes for different growing seasons and for facilitating breeding efforts for high starch-yielding and high-quality cassava starch in the future.

scholarly journals Identification of starch candidate genes using SLAF-seq and BSA strategies and development of related SNP-CAPS markers in tetraploid potato

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261403
Author(s):  
Jiaqi Li ◽  
Xiaoxia Yu ◽  
Sheng Zhang ◽  
Zhuo Yu ◽  
Jingwei Li ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Potato Starch ◽  
Starch Content ◽  
Local Alignment ◽  
Gene Markers ◽  
Potato Varieties ◽  
Tetraploid Potato ◽  
High Starch Content ◽  
High Starch ◽  
Caps Markers

Potato starch is an essential nutrient for humans and is widely used worldwide. Locating relevant genomic regions, mining stable genes and developing candidate gene markers can promote the breeding of new high-starch potato varieties. A total of 106 F1 individuals and their parents (YSP-4 × MIN-021) were used as test materials, from which 20 plants with high starch content and 20 with low starch content were selected to construct DNA pools for site-specific amplified fragment sequencing (SLAF-seq) and bulked segregation analysis (BSA). A genomic region related to the starch traits was first identified in the 0–5.62 Mb of chromosome 2 in tetraploid potato. In this section, a total of 41 non-synonymous genes, which were considered as candidate genes related to the starch trait, were annotated through a basic local alignment search tool (BLAST) search of multiple databases. Six candidate genes for starch (PGSC0003DMG400017793, PGSC0003DMG400035245, PGSC0003DMG400036713, PGSC0003DMG400040452, PGSC0003DMG400006636 and PGSC0003DMG400044547) were further explored. In addition, cleaved amplified polymorphic sequence (CAPS) markers were developed based on single nucleotide polymorphism (SNP) sites associated with the starch candidate genes. SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 were successfully developed and validated with the F2 population and 24 tetraploid potato varieties (lines). Functional analysis and cloning of the candidate genes associated with potato starch will be performed in further research, and the SNP-CAPS markers chr2-CAPS6 and chr2-CAPS21 can be further used in marker-assisted selection breeding of tetraploid potato varieties with high starch content.

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

scholarly journals Starch Production in Chlamydomonas reinhardtii through Supraoptimal Temperature in a Pilot-Scale Photobioreactor

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1084
Author(s):  
Ivan N. Ivanov ◽  
Vilém Zachleder ◽  
Milada Vítová ◽  
Maria J. Barbosa ◽  
Kateřina Bišová
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Starch Content ◽  
Light Availability ◽  
Fold Increase ◽  
Pilot Scale ◽  
Temperature Treatment ◽  
Control Culture ◽  
Starch Accumulation ◽  
Cellular Division ◽  
Starch Production

An increase in temperature can have a profound effect on the cell cycle and cell division in green algae, whereas growth and the synthesis of energy storage compounds are less influenced. In Chlamydomonas reinhardtii, laboratory experiments have shown that exposure to a supraoptimal temperature (39 °C) causes a complete block of nuclear and cellular division accompanied by an increased accumulation of starch. In this work we explore the potential of supraoptimal temperature as a method to promote starch production in C. reinhardtii in a pilot-scale photobioreactor. The method was successfully applied and resulted in an almost 3-fold increase in the starch content of C. reinhardtii dry matter. Moreover, a maximum starch content at the supraoptimal temperature was reached within 1–2 days, compared with 5 days for the control culture at the optimal temperature (30 °C). Therefore, supraoptimal temperature treatment promotes rapid starch accumulation and suggests a viable alternative to other starch-inducing methods, such as nutrient depletion. Nevertheless, technical challenges, such as bioreactor design and light availability within the culture, still need to be dealt with.

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