Total flavonoid contents, antioxidant potential and acetylcholinesterase inhibition activity of the extracts from 15 ferns in China

The current investigation is intended to evaluate the content of phytochemical constituents and antioxidant potential of hydroalcoholic extracts of stem and root of Grewia serrulata DC (HAESGS & HAERGS) and leaf and bark of Grewia Nervosa (Lour.) panigrahi (HAELGN & HAEBGN). Initially, all the extracts at different concentrations were estimated for their total phenolic content and total flavonoid content. The study was further extended for their antioxidant potential evaluation using various in vitro methods such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical and superoxide radical scavenging assays. The total phenolic content (mg gallic acid equivalent per gram of extract) was high in HAELGN (170.82±0.19) and HAERGS (123.00±0.48) than HAESGS (111.2±0.26) and HAEBGN (119.60±0.23). The total flavonoid content (mg quercetin equivalent per gram) is greater in HAERGS (71.24±0.50) and HAESGS (65.68±0.27) than HAELGN (55.82±0.35) and HAEBGN (62.38±0.45). The IC50 values (µg/ml) of different plant extracts inferred that DPPH radical scavenging activity is greater in HAELGN (42.91±0.88) and HAEBGN (53.87±0.35) than HAESGS (126.73±1.20) and HAERGS (88.87±1.25). However, hydroxyl and superoxide radical scavenging activity is more in HAERGS (135.41±1.19 & 88.00±1.42) and HAELGN (172.28±1.91 & 108.163±1.09) than HAESGS (237.3±1.65 & 110.074±0.87) and HAEBGN (204.7±1.04 & 125.54±1.07). The results of present comprehensive analysis demonstrated that both the plants Grewia serrulata DC and Grewia Nervosa (Lour.) panigrahi possess high phenolic, flavonoid contents and potential antioxidant activity, and could be used as a valid source of natural antioxidants and might be utilized for pharmacological screening of various therapeutic activities. Keywords: Grewia serrulata; Grewia Nervosa; Total Phenolic content; Total flavonoid content; Antioxidant potential

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Essential oil extraction from fresh needles of Pinus pumila (Pall.) Regel using a solvent-free microwave-assisted methodology and an evaluation of acetylcholinesterase inhibition activity in vitro compared to that of its main components

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113549 ◽

2021 ◽

Vol 167 ◽

pp. 113549

Author(s):

Xiaojin Peng ◽

Xinyu Yang ◽

Huiyan Gu ◽

Lei Yang ◽

Hongfei Gao

Keyword(s):

Essential Oil ◽

Oil Extraction ◽

Solvent Free ◽

Acetylcholinesterase Inhibition ◽

Inhibition Activity ◽

Pinus Pumila ◽

Microwave Assisted ◽

Main Components

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Synthesis and bioactivity of novel C2-glycosyl triazole derivatives as acetylcholinesterase inhibitors

Heterocyclic Communications ◽

10.1515/hc-2016-0163 ◽

2017 ◽

Vol 23 (3) ◽

pp. 231-236 ◽

Cited By ~ 1

Author(s):

Long Yin ◽

Lei Wang ◽

Xiu-Jian Liu ◽

Feng-Chang Cheng ◽

Da-Hua Shi ◽

...

Keyword(s):

Aqueous Solution ◽

Sodium Hydroxide ◽

Acetylcholinesterase Inhibitors ◽

Acetylcholinesterase Inhibition ◽

Inhibition Activity ◽

Triazole Derivatives ◽

Inhibitory Activities ◽

Ellman’S Method

AbstractNew C2-glycosyl triazole derivatives 6a–l were synthesized by cyclization of glycosyl acylthiosemicarbazides 5 in refluxing 3 N sodium hydroxide aqueous solution. Substrates 5 were obtained by the reaction of glycosyl isothiocyanate 3 with various hydrazides. The acetylcholinesterase (AChE) inhibitory activities of compounds 6 were tested by Ellman’s method. Compounds that exhibited over 85% inhibition were subsequently evaluated for the IC50 values. Compound 6f possesses the best acetylcholinesterase-inhibition activity with IC50 of 1.46±0.25 μg/mL.

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Spectrophotometric determination of sun protective factor (SPF) and antioxidant potential of different solvent extracts of graptophyllum pictum

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v10ispl1.1722 ◽

2019 ◽

Vol 10 (SPL1) ◽

Author(s):

Noor Diyana Ibrahim ◽

Wan Nur Farihah Che Mustafa ◽

Mahendran Sekar ◽

Khor Poh Yen ◽

Seow Lay Jing

Keyword(s):

Total Phenolic Content ◽

Methanol Extract ◽

Phenolic Content ◽

Total Flavonoid ◽

Antioxidant Potential ◽

Aluminium Chloride ◽

Total Phenolic ◽

Uv Spectrophotometry ◽

Total Flavonoid Content ◽

Flavonoid Content

Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present study was to determine the total phenolic and flavonoid contents, Sun Protection Factor (SPF), and antioxidant potential of different solvent extracts of Graptophyllum pictum leaves. The dried powdered leaves were extracted by the cold maceration method, using three different solvents, i.e., methanol, ethanol, and water. The extracted leaves was tested for antioxidant activity using of DPPH (1,1-diphenyl-2-picryl-hydrazyl). Total phenolic content was determined using the Follin-Ciocalteu and for total flavonoid content with used the aluminium chloride. The SPF of all extracts were analyzed by ultraviolet (UV) spectrophotometry. The total phenolic content is the highest in methanol extract (377.61±0.31 µg/ml), and total flavonoid content is the highest in ethanol extract (158.06 ± 0.18 µg/ml). For DPPH free radical scavenging activity, ethanolic extract showed the maximum percentage of inhibition, and for SPF, methanol extract had displayed the highest. The study recommends that methanol is a good solvent for the extraction of sun-protective constituents from G. pictum.

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