Methanol-based γ-aminobutyric acid (GABA) production by genetically engineered Bacillus methanolicus strains

2017 ◽  
Vol 106 ◽  
pp. 12-20 ◽  
Author(s):  
Marta Irla ◽  
Ingemar Nærdal ◽  
Trygve Brautaset ◽  
Volker F. Wendisch
Keyword(s):  
Genetically Engineered ◽  
Aminobutyric Acid ◽  
Gaba Production ◽  
Bacillus Methanolicus

scholarly journals Construction of a Vitreoscilla Hemoglobin Promoter-Based Tunable Expression System for Corynebacterium glutamicum

Catalysts ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 561 ◽  
Author(s):  
Kei-Anne Baritugo ◽  
Hee Taek Kim ◽  
Mi Na Rhie ◽  
Seo Young Jo ◽  
Tae Uk Khang ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Fluorescent Protein ◽  
Expression System ◽  
Vitreoscilla Hemoglobin ◽  
Aminobutyric Acid ◽  
Vector System ◽  
Efficient Production ◽  
Gamma Aminobutyric Acid ◽  
Recombinant Strains ◽  
Gaba Production

Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. glutamicum has various industrial applications, a limited number of tunable systems are available to engineer it for efficient production of platform chemicals. Therefore, in this study, we developed a novel tunable promoter system based on repeats of the Vitreoscilla hemoglobin promoter (Pvgb). Tunable expression of green fluorescent protein (GFP) was investigated under one, four, and eight repeats of Pvgb (Pvgb, Pvgb4, and Pvgb8). The intensity of fluorescence in recombinant C. glutamicum strains increased as the number of Pvgb increased from single to eight (Pvgb8) repeats. Furthermore, we demonstrated the application of the new Pvgb promoter-based vector system as a platform for metabolic engineering of C. glutamicum by investigating 5-aminovaleric acid (5-AVA) and gamma-aminobutyric acid (GABA) production in several C. glutamicum strains. The profile of 5-AVA and GABA production by the recombinant strains were evaluated to investigate the tunable expression of key enzymes such as DavBA and GadBmut. We observed that 5-AVA and GABA production by the recombinant strains increased as the number of Pvgb used for the expression of key proteins increased. The recombinant C. glutamicum strain expressing DavBA could produce higher amounts of 5-AVA under the control of Pvgb8 (3.69 ± 0.07 g/L) than the one under the control of Pvgb (3.43 ± 0.10 g/L). The average gamma-aminobutyric acid production also increased in all the tested strains as the number of Pvgb used for GadBmut expression increased from single (4.81–5.31 g/L) to eight repeats (4.94–5.58 g/L).

scholarly journals Gamma-aminobutyric acid-producing lactobacilli positively affect metabolism and depressive-like behaviour in a mouse model of metabolic syndrome

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
E. Patterson ◽  
P. M. Ryan ◽  
N. Wiley ◽  
I. Carafa ◽  
E. Sherwin ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Mouse Model ◽  
Plasma Cholesterol ◽  
Forced Swim Test ◽  
Lactobacillus Brevis ◽  
Aminobutyric Acid ◽  
Metabolic Dysfunction ◽  
Gamma Aminobutyric Acid ◽  
Gaba Production ◽  
Glucose Challenge

Abstract Metabolic and neuroactive metabolite production represents one of the mechanisms through which the gut microbiota can impact health. One such metabolite, gamma-aminobutyric acid (GABA), can modulate glucose homeostasis and alter behavioural patterns in the host. We previously demonstrated that oral administration of GABA-producing Lactobacillus brevis DPC6108 has the potential to increase levels of circulating insulin in healthy rats. Therefore, the objective of this study was to assess the efficacy of endogenous microbial GABA production in improving metabolic and behavioural outcomes in a mouse model of metabolic dysfunction. Diet-induced obese and metabolically dysfunctional mice received one of two GABA-producing strains, L. brevis DPC6108 or L. brevis DSM32386, daily for 12 weeks. After 8 and 10 weeks of intervention, the behavioural and metabolic profiles of the mice were respectively assessed. Intervention with both L. brevis strains attenuated several abnormalities associated with metabolic dysfunction, causing a reduction in the accumulation of mesenteric adipose tissue, increased insulin secretion following glucose challenge, improved plasma cholesterol clearance and reduced despair-like behaviour and basal corticosterone production during the forced swim test. Taken together, this exploratory dataset indicates that intervention with GABA-producing lactobacilli has the potential to improve metabolic and depressive- like behavioural abnormalities associated with metabolic syndrome in mice.

Enzymatic Production of γ-Aminobutyric Acid Using Rice Bran

2012 ◽  
Vol 586 ◽  
pp. 85-91
Author(s):  
Ying Guo Lü ◽  
Hui Zhang ◽  
Hui Yuan Yao
Keyword(s):  
Reaction Time ◽  
Rice Bran ◽  
Glutamate Decarboxylase ◽  
Monosodium Glutamate ◽  
Aminobutyric Acid ◽  
Enzymatic Method ◽  
Enzymatic Production ◽  
Gaba Production ◽  
Gaba Content ◽  
Time Buffer

An enzymatic method for γ-aminobutyric acid (GABA) production was invested. With this method, rice bran was used as glutamate decarboxylase (GAD) source and exogenous monosodium glutamate(MSG) was used as substrate. We stimulated the rice bran GAD via regulating the temperature, pH, reaction time, buffer and adding PLP, Ca2+ and substrate. In the existence of PLP and Ca2+, the GABA content of rice bran had been improved by about 45 fold. The GABA production reached 2.3g/100g bran, and the Glu conversion reached 100%. As rice bran is a by-product in rice processing and a large quantity of rice bran is commercially available, our study illuminated a safe and efficient way to produce GABA and GABA enriched food.

Study on conditions for Gama buryric acid (GABA) production in Lactobacillus fermentum A01 isolated from human

2021 ◽  
pp. 2188-2190
Author(s):  
Cat Thien Phuc ◽  
Doan Thi Thanh Vinh ◽  
Truong Khoa My ◽  
Tran Thi Thu Hien ◽  
Nguyen Anh Dung ◽  
...  
Keyword(s):  
High Performance ◽  
Hplc Analysis ◽  
High Efficiency ◽  
Monosodium Glutamate ◽  
Vital Role ◽  
Lactobacillus Fermentum ◽  
Aminobutyric Acid ◽  
Optimal Conditions ◽  
Gaba Production ◽  
Layer Chromatography

Lactic acid bacteria play a vital role in biosynthesis of γ -aminobutyric acid (GABA) in the presence of glutamic acid - major substrate for the process. In recent study, Lactobacillus fermentum A01 (L. fermentum A01) isolated from various sources in Vietnam were screened for bacteria strains with high efficiency in GABA formation. L. fermentum was cultured in MRS broth containing 25 mg/mL monosodium glutamate (MSG), at pH of 6.5 and incubated at the optimal conditions (37ºC, for 24, 48, 72 h). After extraction and thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) analysis, L. fermentum A01 showed the GABA yield about 1.34 mg/g in dried supernatant, suggesting L. fermentum A01 to be a promising GABA producer for food and pharmaceutical applications.

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