Transcriptome profiling of Jerusalem artichoke seedlings (Helianthus tuberosus L.) under polyethylene glycol-simulated drought stress

Jerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions. Methods: Ultra-performance liquid chromatography and tandem mass spectrometry were used to identify differential metabolites in Jerusalem artichoke seedling leaves under polyethylene glycol (PEG) 6000-simulated drought stress at 0, 18, 24, and 36 h. Results: A total of 661 metabolites and 236 differential metabolites were identified at 0 vs. 18, 18 vs. 24, and 24 vs. 36 h. 146 differential metabolites and 56 common were identified and at 0 vs. 18, 24, and 36 h. Kyoto Encyclopedia of Genes and Genomes enrichment identified 236 differential metabolites involved in the biosynthesis of secondary metabolites and amino acids. Metabolites involved in glycolysis, phenolic metabolism, tricarboxylic cycle, glutamate-mediated proline biosynthesis, urea cycle, amino acid metabolism, unsaturated fatty acid biosynthesis, and the met salvage pathway responded to drought stress. Conclusion: A metabolic network in the leaves of Jerusalem artichokes under drought stress is proposed. These results will improve understanding of the metabolite response to drought stress in Jerusalem artichokes and develop a foundation for breeding drought-resistant varieties.

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Drought tolerance molecular responses of two cultivated varieties Jerusalem artichoke (Helianthus tuberosus L.) as revealed by RNA-Seq

10.21203/rs.3.rs-124586/v1 ◽

2020 ◽

Author(s):

Shipeng Yang ◽

Lihui Wang ◽

Qiwen Zhong ◽

Guangnan Zhang ◽

Haiwang Zhang ◽

...

Keyword(s):

Drought Stress ◽

Drought Resistance ◽

Metabolic Pathways ◽

Jerusalem Artichoke ◽

Tibet Plateau ◽

Helianthus Tuberosus ◽

Rna Seq ◽

Concentration Gradients ◽

Light Reaction ◽

Metabolic Activities

Abstract Background Jerusalem artichoke (Helianthus tuberosus L.) is a highly stress-resistant crop, especially it grows normally in the desertified land of Qinghai-Tibet Plateau in the past two years, and has become a crop with agricultural, industrial and ecological functions. However, there are few studies on drought resistance of Jerusalem artichoke at present, and studies on the mechanisms of stress resistance of Jerusalem artichoke breeding and fructan are seriously lagging behind. In this study, we selected two differentially resistant cultivars for drought stress experiments with different concentration gradients, the aim was finding DEGs and metabolic pathways associated with drought stress. Results Based on an additional analysis of the metabolic pathways under drought stress using MapMan, the most different types of metabolism included secondary metabolism, light reaction metabolism and cell wall. As a whole, QY1 and QY3 both had a large number of up-regulated genes in the flavor pathway. It was suggested that flavonoids could help Jerusalem artichoke to resist drought stress and maintain normal metabolic activities. In addition, the gene analysis of the abscisic acid (ABA) key metabolic pathway showed that QY3 had more genes in NAC and WRKY than QY1, but QY1 had more genes in response to drought stress as a whole. By combining RNA-Seq and WGCNA, a weighted gene co-expression network was constructed and divided into modules. By analyzing specifically the expressed modules, four modules were found to have the highest correlation with drought. Further research on the genes revealed that all 16 genes related to histone, ABA and protein kinase had the highest significance in these pathways. Conclusions These findings represent the first RNA-Seq analysis of drought stress in Jerusalem artichoke, which is of substantial significance to explore the function of drought resistance in Jerusalem artichoke and the excavation of related genes.

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Modulating the antioxidant system by exogenous 2-(3,4-dichlorophenoxy) triethylamine in maize seedlings exposed to polyethylene glycol-simulated drought stress

PLoS ONE ◽

10.1371/journal.pone.0203626 ◽

2018 ◽

Vol 13 (9) ◽

pp. e0203626 ◽

Cited By ~ 2

Author(s):

Tenglong Xie ◽

Wanrong Gu ◽

Liguo Zhang ◽

Lijie Li ◽

Danyang Qu ◽

...

Keyword(s):

Polyethylene Glycol ◽

Drought Stress ◽

Antioxidant System ◽

Maize Seedlings ◽

Simulated Drought

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A fructan: the fructan 1-fructosyl-transferase gene from Helianthus tuberosus increased the PEG-simulated drought stress tolerance of tobacco

Hereditas ◽

10.1186/s41065-020-00131-3 ◽

2020 ◽

Vol 157 (1) ◽

Author(s):

Xuemei Sun ◽

Yuan Zong ◽

Shipeng Yang ◽

Lihui Wang ◽

Jieming Gao ◽

...

Keyword(s):

Drought Stress ◽

Stress Tolerance ◽

Helianthus Tuberosus ◽

Drought Stress Tolerance ◽

Transferase Gene ◽

Simulated Drought

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Ethylene glycol tetra-acetic acid and salicylic acid improve anti-oxidative ability of maize seedling leaves under heavy-metal and polyethylene glycol 6000-simulated drought stress

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263504.2013.878408 ◽

2014 ◽

Vol 148 (1) ◽

pp. 96-108 ◽

Cited By ~ 8

Author(s):

W. Y. Song ◽

S. P. Peng ◽

C. Y. Shao ◽

H. B Shao ◽

H. C. Yang

Keyword(s):

Heavy Metal ◽

Acetic Acid ◽

Salicylic Acid ◽

Polyethylene Glycol ◽

Drought Stress ◽

Ethylene Glycol ◽

Maize Seedling ◽

Simulated Drought ◽

Polyethylene Glycol 6000

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Transcriptome changes induced by drought stress in Jerusalem artichoke (Helianthus tuberosus L.) and weighted gene co-expression network analysis (WGCNA)

10.21203/rs.3.rs-25678/v1 ◽

2020 ◽

Author(s):

Shipeng Yang ◽

Lihui Wang ◽

Qiwen Zhong ◽

Guangnan Zhang ◽

Dengshan Zhang ◽

...

Keyword(s):

Drought Stress ◽

Drought Resistance ◽

Jerusalem Artichoke ◽

Northern China ◽

Qinghai Lake ◽

Helianthus Tuberosus ◽

Rna Seq ◽

Light Reaction ◽

Qinghai Province ◽

Watering Treatment

Abstract Background: Jerusalem artichoke (Helianthus tuberosus L.) is strongly resistant to stress and an important plant used for ecological management in northern China in recent years. Currently, Jerusalem artichoke has been widely planted in the area around Qinghai Lake in Qinghai Province, China. Jerusalem artichoke can not only prevent land desertification but also has maintain most of its level of production. However, there is little research on the mechanism of drought resistance of Jerusalem artichoke.Results: We conducted transcriptome sequencing under drought stress and normal watering treatment for two varieties, QY1 and QY3, with differing degrees of drought tolerance. In the three stress periods of QY1 and QY3, 5,613, 12,985 and 24,923 differentially expressed genes (DEGs) were identified, respectively. GO analysis showed that there were more DEGs in QY1 than in QY3, but there were more up-regulated genes in QY3 than in QY1. Based on an additional analysis of the metabolic pathways under drought stress using MapMan, the most different types of metabolism included secondary metabolism, light reaction metabolism and cell wall. The up-regulated genes in QY3 were significantly more prevalent than those in QY1 and were primarily concentrated in flavor IDS, phenylpropanoids, and the shikimate and terpenoids pathway. As a whole, QY1 and QY3 both had a large number of up-regulated genes in the flavor pathway. In addition, the gene analysis of the ABA key metabolic pathway showed that QY3 had more genes in NAC and WRKY than QY1. A weighted gene co-expression network was constructed and divided into modules. By specifically analyzing the expressed modules, four modules were found to have the highest correlation with drought. Further research on the genes revealed that all 16 genes related to histone, ABA and protein kinase were the most significant in these pathways.Conclusions: In summary, these findings represent the first RNA-Seq analysis of drought stress in Jerusalem artichoke, which is of substantial significance to explore the function of drought resistance in Jerusalem artichoke and the unearthing of related genes.

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