In vitro germination, storage and microscopic studies of pollen grains of four Ocimum species

2022 ◽  
Vol 177 ◽  
pp. 114445
Author(s):  
Mamta Kumari ◽  
Archana Prasad ◽  
Laiq ur Rahman ◽  
Ajay Kumar Mathur ◽  
Archana Mathur
Keyword(s):  
Pollen Grains ◽  
In Vitro Germination ◽  
Ocimum Species ◽  
Microscopic Studies

scholarly journals Pecan Pollen Stored Over a Decade Retains Viability

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 176-177 ◽  
Author(s):  
Darrell Sparks ◽  
I.E. Yates
Keyword(s):  
Liquid Nitrogen ◽  
Pollen Morphology ◽  
Germ Tube ◽  
Pollen Grains ◽  
Carya Illinoinensis ◽  
In Vitro Germination ◽  
Fresh Pollen

In vitro germination of freshly collected pollen and pollen stored 1, 10, 11, 12, and 13 years in liquid nitrogen was examined for `Desirable' pecan [Carya illinoinensis (Wangenh.) C. Koch]. Viability of pollen stored in liquid nitrogen for 1, 10, 11, 12, and 13 years was not diminished in comparison to that of fresh pollen. Morphology of stored pollen grains and the germ tube was normal. Thus, liquid nitrogen may offer a means of haploid preservation of pecan.

scholarly journals Optimization of In Vitro Pecan Pollen Germination

HortScience ◽  
2011 ◽  
Vol 46 (4) ◽  
pp. 571-576 ◽  
Author(s):  
Patrick J. Conner
Keyword(s):  
Polyethylene Glycol ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Solid Support ◽  
Carya Illinoinensis ◽  
Testing System ◽  
In Vitro Germination ◽  
Ethanesulfonic Acid ◽  
Made In

Storage of pollen from 1 year to the next is often needed to enable desired crosses to be made in a pecan [Carya illinoinensis (Wangenh.) K. Koch] breeding program. Stored pollen is usually tested for viability through the use of in vitro germination tests. An in vitro germination testing system was developed for this purpose using cellophane booklets to provide a solid support for the pollen grains. Optimized germination media contained 5% sucrose, 20% polyethylene glycol 8000, 0.05% Ca(NO3)2, 0.025% H3BO3, and 10 mm 2-(N-morpholino)ethanesulfonic acid pH 6.0. Pollen should be rehydrated for 2 to 4 h in a humidified chamber before germination testing. A germination time of 4 to 24 h produces similar final germination percentages. Testing of pollen samples stored at –80 °C indicates that pecan pollen can be stored for at least 8 years without a decrease in viability. Chemical names used: polyethylene glycol (PEG); 2-(N-morpholino)ethanesulfonic acid (MES).

scholarly journals Viability of pollen grains and stigma receptivity in Desert Rose

2022 ◽  
Vol 28 (1) ◽  
pp. 92-98
Author(s):  
Cristiane Gonçalves Souza ◽  
Sabrina Maihave Barbosa Ramos ◽  
Silvia Nietsche ◽  
Clivia Carolina Fiorilo Possobom ◽  
Elka Fabiana Aparecida Almeida ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Pollen Viability ◽  
Oxygen Demand ◽  
Pollen Grains ◽  
Floral Biology ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Stigma Receptivity ◽  
Flower Opening

Abstract Adenium obesum (Forssk.) Roem. & Schult., popularly known as desert rose, has become a valuable ornamental plant. In floriculture, the production of hybrids is prioritized. Hence, knowledge on floral biology and sexual reproduction of the target species is fundamental. The objectives of this study were: (1) to test sucrose concentrations and temperatures for in vitro germination of A. obesum pollen grains; (2) to identify the effect of temperature on the viability of A. obesum pollen grains; and (3) to evaluate the viability of pollen grains and stigma receptivity in pre-anthesis, at flower opening, and 72 h post-flower opening in three accessions of A. obesum. A significant relationship between temperatures and sucrose concentrations was observed in the in vitro germination test. The highest percentage of in vitro germination of pollen grains, 39.81%, was observed at an estimated temperature of 26.05 °C. Desert rose accessions maintained in biochemical oxygen demand (BOD) chambers at 30 °C during a 16-h light photoperiod showed faster flowering, and temperatures ≥ 25 °C induced pollen grain viability percentages above 69%. Temperature is one of the most important abiotic factors, influencing mainly in pollen germination, pollen tube growing and in efficiency fertilization. The ICA-wd accession stood out and can be considered a pollen donor in artificial pollination. The stigmas of flowers were receptive from a day before flower opening until three days after. The two parameters presented above, stigma receptivity and pollen viability, allow inferences about the appropriate time for successful pollination and subsequent fertilization in desert roses.

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

scholarly journals In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)

2013 ◽  
Vol 35 (4) ◽  
pp. 1116-1126 ◽  
Author(s):  
Taliane Leila Soares ◽  
Onildo Nunes de Jesus ◽  
Janay Almeida dos Santos-Serejo ◽  
Eder Jorge de Oliveira
Keyword(s):  
Pollen Germination ◽  
Culture Medium ◽  
Pollen Viability ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Triphenyltetrazolium Chloride ◽  
Histochemical Analysis ◽  
High Viability ◽  
Three Stages

The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.

In vitro germination and pollen tube growth of maize (Zea mays) pollen. IX. Pollen source genotype and nonionic surfactant interactions

1980 ◽  
Vol 58 (5) ◽  
pp. 557-561 ◽  
Author(s):  
P. L. Pfahler ◽  
H. F. Linskens ◽  
M. Wilcox
Keyword(s):  
Pollen Tube ◽  
Calcium Nitrate ◽  
Pollen Grains ◽  
Tween 80 ◽  
Tube Length ◽  
In Vitro Germination ◽  
Sorbitan Monooleate ◽  
Germination Characteristics ◽  
Surfactant Effects

Because of their structure and active metabolic state, pollen grains are vulnerable to any agent in the in vitro germination medium. Surfactants are used in biological systems to reduce surface tension but are also known to produce subtle biochemical effects. This study investigated the effect of three nonionic surfactants on the germination characteristics of pollen grains from three pollen source genotypes. Pollen grains from three single cross hybrids (Wf9 × H55, K64 × K55, Ky228 × Ky226) were cultured on an artificial medium (15% sucrose, 0.6% bacto-agar, 0.03% calcium nitrate, 0.01% boric acid) supplemented with all possible combinations of three concentrations (10, 100, 1000 ppm, v/v) of the following surfactants: (i) Tween 80 (polyoxyethylene sorbitan monooleate); (ii) X-114 (alkyl phenoxypolyethoxy ethanol); and (iii) commercial sticker spreader (alkyl olefin aromatic polymers). A control containing no supplement was included. Over all genotypes, increasing concentrations of Tween 80 had the least effect on the germination characteristics measured (germination and ruptured percent, pollen tube length at 1, 2, and 3 h after inoculation) and X-114 had the greatest effect with no germination found above 10 ppm. For most of the germination characteristics, highly significant pollen source genotype X concentration interactions were found for each surfactant. The direction and magnitude of the surfactant effects depended on the surfactant, its concentration, the germination characteristic involved, and the pollen source genotype.

In vitro Germination of Cotton Pollen Grains Using two Different Approaches

2000 ◽  
Vol 3 (10) ◽  
pp. 1591-1592 ◽  
Author(s):  
Mohammad Jurial Baloch ◽  
Abdul Rahim Lakho ◽  
Hidayatullah Bhutto ◽  
Rahmatullah Rind
Keyword(s):  
Pollen Grains ◽  
In Vitro Germination

Optimization of culture medium for the in vitro germination and histochemical analysis of Passiflora spp. pollen grains

2021 ◽  
Vol 288 ◽  
pp. 110298
Author(s):  
Michele dos Santos Ferreira ◽  
Taliane Leila Soares ◽  
Eva Maria Rodrigues Costa ◽  
Ronilze Leite da Silva ◽  
Onildo Nunes de Jesus ◽  
...  
Keyword(s):  
Culture Medium ◽  
Pollen Grains ◽  
In Vitro Germination ◽  
Histochemical Analysis

scholarly journals Determination of cardinal temperatures for sugar apple ( Annona squamosa L.)

2016 ◽  
Vol 40 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Bruno Rafael Alves Rodrigues ◽  
Rayane Carneiro dos Santos ◽  
Silvia Nietsche ◽  
Maria Olívia Mercadante-Simões ◽  
Isabella Renata Gomes da Cunha ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Cardinal Temperatures ◽  
Germination Of Pollen

ABSTRACT The sugar apple is highly dependent on the pollination and fruit tree pollen performance is crucial for successful fertilization and fruit set. The objective of this study was to determine the cardinal temperatures for in vitro pollen grains germination and evaluate the effect of temperature on pollen tube growth of sugar apple. The experimental design was in a completely randomized with eight treatments (10, 15, 20, 25, 30, 35, 40 and 45 ºC), four replicates and each plot was constituted by two slides per parcel. The cardinal temperatures were determined by evaluating in vitro germination of pollen grains and pollen tube growth in standard culture medium. It also assessed the pollen tube growth and the percentage of germination in vitro depending on the type of pollen grain arrangement (monad, dyad, triad and tetrad). In vitro germination of pollen grains and pollen tube growth varied significantly with temperature. The maximum germination recorded (48.13%) and the maximum lengths of pollen tubes (536.45 μm) were obtained when pollen grains were cultivated at 25 ºC. The estimated cardinal temperatures were 9.7, 26.9 and 44.2 ºC. Among the pollen grain arrangements, tetrads pollen grains were observed in higher proportions, however, monads pollen grains presented higher germination percentage.

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