Virucidal activity of Moringa A from Moringa oleifera seeds against Influenza A Viruses by regulating TFEB

Abstract BackgroundInfluenza A viruses (IAVs) are highly contagious pathogens infecting human and numerous animals. The viruses cause millions of infection cases and thousands of deaths every year, thus making IAVs a continual threat to global health. MethodsMoringa A was isolated from Moringa oleifera seeds and tested for antiviral activity against H1N1. The antiviral activity of Moringa A was tested by checking their effect on hemagglutination and PFU activities of the studied virus, and the cytopathic effect was observed too. Additionally, the different types of treatment experiments were performed to complement the analysis of the antiviral activity of Moringa A, and the contents of inflammatory cytokines and the expression of TFEB were detected.ResultsMoringa A inhibits virus replication in host cells, and it protects infected cells from cytopathic effect induced by IAVs. The EC50 and EC90 value of Moringa A for IAVs were 1.27 and 5.30 μM, respectively. The different types of treatment experiments revealed that Moringa A has a significant inhibitory effect on the IAVs both before and after drug addition. What’s more, Moringa A was observed to decrease the levels of inflammatory cytokines TNF-α, IL-6, IL-1β and IFN-β in H1N1 infected RAW264.7 cells. Finaly, Moringa A was found to inhibit the expression and nuclear transfer of the cellular protein transcription factor EB (TFEB), and weaken the autophagy in infected cells, which could be an important antiviral mechnism of Moringa A. ConclusionsMoringa A has potent antiviral activity against IVAs, which could be due to the autophagy inhibition property.

Download Full-text

Molecular investigation of the virucidal activity of proanthocyanidin from Alpinia zerumbet against the influenza A virus

10.21203/rs.3.rs-970727/v1 ◽

2021 ◽

Author(s):

Hayato Morimoto ◽

Tadashi Hatanaka ◽

Mari Narusaka ◽

Yoshihiro Narusaka

Keyword(s):

Membrane Proteins ◽

Influenza A ◽

Direct Interaction ◽

Virucidal Activity ◽

Influenza A Viruses ◽

Viral Membrane ◽

Diarrhea Virus ◽

Anti Oxidant ◽

Influenza Virus Replication ◽

Alpinia Zerumbet

Abstract Proanthocyanidins (PACs) have various bioactivities, such as being anti-bacterial, anti-cancer, and anti-oxidant. Consequently, they have been vigorously studied for the development of new natural bioactive compounds. Recently, AzPAC was isolated from the medicinal plant Alpinia zerumbet, and it was found to inhibit the infection of animal viruses, influenza A viruses (IAVs), and porcine epidemic diarrhea virus. The virucidal activity of AzPAC means that it can interact directly with viral particles. However, few studies have investigated the preventive mechanism utilized by AzPAC on influenza virus replication. In this study, the composition of AzPAC and the affinity between AzPAC and IAVs was investigated in detail. We found that AzPAC was composed of an epicatechin monomer, which was linked by inter-flavan bonds between the C4 and C8 positions (B2-type) and the C4 and C6 positions (B5-type) in the terminal units of the PAC. A quenching assay indicated that AzPAC interacted with IAV membrane proteins, hemagglutinin and neuraminidase. Additionally, circular dichroism analysis indicated that AzPAC affected the change in the secondary structure rate of the viral membrane proteins. AzPAC was able to impair the infective process of IAVs via direct interaction with their viral membrane proteins. These results indicate that A. zerumbet is an invaluable bioresource for the development of preventive drugs against IAV infection.

Download Full-text

Study of the medicine MestaMidin®-nos virucidal activity of against influenza and ARD pathogens

Medical Council ◽

10.21518/2079-701x-2021-12-174-180 ◽

2021 ◽

pp. 174-180

Author(s):

A. A. Shtro ◽

A. V. Galochkina ◽

Yu. V. Nikolaeva ◽

G. D. Petukhova ◽

V. A. Fedorova ◽

...

Keyword(s):

Influenza A ◽

Respiratory Infections ◽

Rapid Development ◽

Organic Pollution ◽

The Body ◽

Influenza B Virus ◽

Influenza B ◽

Virucidal Activity ◽

Influenza A Viruses ◽

Spread Of Infection

Introduction. Respiratory diseases have always been a serious threat to public health, but in 2020 the situation deteriorated significantly due to the rapid development of the coronavirus infection COVID-19. Due to the lack of available means of ethiotropic therapy and the still insufficient coverage of the general population with vaccination, disinfectants, as well as topical preparations that prevent the penetration of the virus into the body, play an important role in preventing the spread of infection.Aim of the study. This work is devoted to the study of the virucidal activity of the medicine Mestamidin-nos against respiratory viruses, namely influenza A and B viruses, parainfluenza, respiratory syncytial, adenovirus and seasonal coronavirus.Materials and methods. This study was carried out by the suspension method according to MU 3.5.2431-08 “Study and assessment of the virucidal activity of disinfectants”.Results. It was shown that for all studied viruses the use of the compound for 60 minutes led to a complete (up to 0 lgTID50) or sufficient (4 lgTID50) decrease in the viral titer. In the case of a study of a compound with a protein load (imitating strong organic pollution), the effectiveness of the compound was significantly reduced against influenza A viruses, one of the strains of influenza B virus, adenovirus and seasonal coronavirus.Conclusion. Based on the results of the study, it can be concluded that the effective inhibition of pathogens of influenza and acute respiratory infections using the compound MesaMidin®-nos in the absence of strong organic pollution.

Download Full-text