IC-P-105: Apoptosis is a defensive mechanism against the probable damages of chronic inflammation induced by Aβ in hippocampus in male rats

2009 ◽  
Vol 5 (4S_Part_2) ◽  
pp. P45-P45
Author(s):  
Azadeh Abdi ◽  
Abolhassan Ahmadiani
Keyword(s):  
Chronic Inflammation ◽  
Male Rats ◽  
Defensive Mechanism

Prevention of Intra-Abdominal Adhesions Using the Combination of Mediclore® and a Statin

2021 ◽  
pp. 1-9
Author(s):  
Jesung Park ◽  
Hyun Kang ◽  
Yoo Shin Choi ◽  
Suk-Won Suh ◽  
Soon Auck Hong ◽  
...  
Keyword(s):  
Chronic Inflammation ◽  
Acute Inflammation ◽  
Adhesion Formation ◽  
Male Rats ◽  
Control Group ◽  
Fibrosis Score ◽  
Abdominal Adhesions ◽  
Group Assignment ◽  
Control Group Group ◽  
Inflammation Score

<b><i>Purpose:</i></b> This study investigated the antiadhesive effects of Mediclore®, rosuvastatin, and a combination of Mediclore and rosuvastatin in a rat adhesion model. <b><i>Methods:</i></b> The adhesion models (a total of 58 adult male rats) were divided into 4 groups. The control group (group C) received no special materials except for a saline. The experimental groups were treated with 5 mL of Mediclore (group M), rosuvastatin (group R), or rosuvastatin and Mediclore (group RM), and these materials were intraperitoneally placed under the incision. At postoperative day 14, the rats underwent re-laparotomy and adhesiolysis. Three investigators blinded to group assignment scored the extent of adhesion formation, the numbers of remote adhesions, and the extent of acute/chronic inflammation, fibrosis, edema, and congestion on resected specimens via histologic examination. <b><i>Results:</i></b> The macroscopic adhesion score in group RM (7.27 ± 3.51) was significantly lower than those in groups C (13.36 ± 2.24) and R (11.71 ± 1.98); group M (9.13 ± 4.09) had a significantly lower adhesion score than group C. The number of remote adhesions was significantly lower in groups R and RM than in group C. The acute inflammation score, chronic inflammation score, and fibrosis score in group RM; the acute inflammation score in group R; and the fibrosis score in group M were significantly lower than those in group C. <b><i>Conclusion:</i></b> The intraperitoneal application of Mediclore and a combination of Mediclore and rosuvastatin effectively reduced postoperative adhesions.

Correlational analysis of the regulatory interplay between molecules and cellular components mediating angiogenesis in wound healing under normal and hyperglycemic conditions

2021 ◽  
pp. 1-12
Author(s):  
Larysa Natrus ◽  
Irina Ryzhko ◽  
Arturo Blazquez-Navarro ◽  
Tatyana Panova ◽  
Ganna Zaychenko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Inflammation ◽  
Peripheral Blood ◽  
Relative Number ◽  
Kinetic Studies ◽  
Receptor Expression ◽  
Male Rats ◽  
Burn Wound ◽  
High Association ◽  
Blood Neutrophils

AIM: The aim of this study was to correlate the content of cells with regulatory molecules associated with angiogenesis in wound healing in a rat model of hyperglycemia. We hypothesize that blood neutrophils are the main VEGF source and can stimulate FLT-1 receptor expression, which is the perquisite for efficient neoangiogenesis. MATERIALS AND METHODS: Kinetic studies of the healing dynamics (3, 7, 14, 21 days) of burn wounds on the skin were conducted in white adult male rats. The content of nuclear factor kappa B (NF-κB), vascular endothelial growth factor (VEGF), its receptor (Flt-1) in the regenerated tissue was analyzed by western blot. Numbers of cells associated with the regenerative process and from peripheral blood (PB) were determined. Additionally a bone marrow (BM) myelogram was conducted. RESULTS: The relative number of peripheral blood (PB) neutrophils was found to be associated with the level of VEGF (R = 0.708) and Flt-1 (R = 0.472). The relative number of fibroblasts was also associated with VEGF (R = 0.562), but not with Flt-1. A negative association was found between the number of neutrophils in the regenerated tissue with VEGF (R = –0.454) and FLT-1 (R = –0.665). This confirms our hypothesis, that blood neutrophils are the main VEGF producer that stimulate the expression of the FLT-1 receptor subsequently inducing neoangiogenesis. Furthermore, that under hyperglycemic conditions fibroblasts were highly associated with VEGF (R = 0.800), while negatively associated with FLT-1 (R = –0.506). There was a high association between PB neutrophils and newly generated tissue cells: neutrophils (R = 0.717) and macrophages (R = 0.622), as well as the association between neutrophils and macrophages (R = 0.798). This is an indication of chronic inflammation and increased transmigration of blood cells to the burned tissue. CONCLUSION: Blood neutrophils are the main producer of VEGF and stimulate the expression of the FLT-1 receptor. In the context of hyperglycemia the imbalance of receptor and ligand associated with angiogenesis indicates for chronic inflammation: VEGF and FLT-1, which facilitates hypoxia, prevents the physiological course of burn wound healing and may be important factors in impaired tissue regeneration in diabetes.

scholarly journals Effect of Smoke Exposure on Chronic Inflammation and P53 Expression in Pelvis Epithelial

2020 ◽  
Vol 9 (3S) ◽  
pp. 282-288
Keyword(s):  
Cigarette Smoking ◽  
Chronic Inflammation ◽  
Lymphocyte Count ◽  
Correlation Study ◽  
Upper Urinary Tract ◽  
Smoke Exposure ◽  
Male Rats ◽  
P53 Expression ◽  
Smoking Exposure ◽  
Cigarette Smoking Exposure

Many risk factors can cause upper urinary tract carcinoma. Smoking is the most influential risk factor and is associated with the formation of aromatic acids and could induce apoptotic cycle. This research was aimed to examine the effect of cigarete smoke exposure on inflammatory state and p53 expression in renal pelvis epithelial of wistar rats. As many as 25 male rats aged 6-8 weeks were divided into five groups as follows: Control (without treatment); CS-1, CS-2, CS-4, and CS-8 (treated with CS 1x, 2x, 4x, and 8x/day, respectively). Each exposure was done for 15 minutes for 60 days. histopathological changes were evaluated from HE-stained specimens and immunohistochemistry method was applied to measure p53 expressio neutrophile and lymphocyte count in CS-8 was significantly higher as compared to CS-1 (p<0.05) and control (p<0.05). p53 expression was found in the CS-8 (3 out of 5 subjects had positive p53 expression, with a total of 4-7 cells from 10 hpf). Correlation study showed significant correlation between frequency of cigarette smoking exposure and neutrophile count (p=0.000; r=-0.856); lymphocyte count (p=0.000; r=0.985); and p53 expression (p=0.000; 0.072). We concluded that there were significant differences in acute inflammation state, chronic inflammation state and p53 expression among groups. Correlation study showed that frequency of cigarette smoking exposure was positively correlated with lymphocyte count and p53 expression.

scholarly journals The Short-Term Effect of Dried Plums on Inflammation and Indices of Bone Health in Osteopenic Men (P01-027-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Kelli George ◽  
Neda Akhavan ◽  
Lauren Ormsbee ◽  
Joseph Munoz ◽  
Elizabeth Foley ◽  
...  
Keyword(s):  
Bone Loss ◽  
Chronic Inflammation ◽  
Dietary Intervention ◽  
Trabecular Bone Score ◽  
Lumbar Vertebrae ◽  
Male Rats ◽  
Control Group ◽  
Mineral Density ◽  
Regular Consumption ◽  
One Year

Abstract Objectives Osteoporosis has significant public health importance for both women and men, where 54% of the U.S. population age 50 and older have low bone mineral density (BMD). Chronic inflammation alters bone remodeling, which is one contributor to bone loss; therefore, foods rich in antioxidants, such as dried plums (DP, Prunus domestica L.), are of great interest for preventing chronic inflammation. Previously, dietary intervention with DP has been shown to prevent orichidectomy-induced decreases in BMD, microstructure, and biomechanics in male rats; however, this has yet to be studied in a clinical setting in adult males. Methods One-hundred and sixty osteopenic men are being recruited from the greater Tallahassee, Florida area to examine the effects of DP on BMD, bone biomarkers, and inflammation after one year of regular consumption. The present analysis is of a subset of this population, documenting the effects of two doses of DP on biomarkers of inflammation and bone metabolism in men after three months of consumption. Twenty-seven men between the ages of 55 and 80 with moderate bone loss (T-score between −0.1 and −2.5 SD below the mean) were included. The men were randomized into one of three groups: 100 g DP, 50 g DP, or control group, with all three groups given a multivitamin containing 450 mg calcium and 800 IU vitamin D (Shaklee Corporation). Serum samples from the baseline and three-month time points were analyzed for C-reactive protein (CRP) and bone-specific alkaline phosphatase (BAP). DXA scans of the lumbar vertebrae alongside TBS iNnsight® software were used to generate trabecular bone score (TBS). Results Three months of DP consumption was associated with numerical increases in BAP in both the 100 g (6.5%, P = 0.14) and 50 g (6.3%, P = 0.3) DP groups, numerical decreases in CRP in both the 100 g (−8.8%, P = 0.75) and 50 g (−8.5%, P = 0.71) DP groups, and minimal change in TBS in both the 100 g (0.37%, P = 0.71) and 50 g (−0.55%, P = 0.44) DP groups. Conclusions Regular consumption of either 100 g or 50 g DP for three months may contribute to increases in bone formation and decreases in inflammation, however not to an extent that affects bone quality. Three months of consumption may not be long enough to manifest changes in bone; therefore, further analysis of data after six months and one year of DP consumption in a larger number of men is warranted. Funding Sources USDA-NIFA, Shaklee Corporation, California Dried Plum Board.

Spinal Cord Brain-Derived Neurotrophic Factor Levels Increase after Dexamethasone Treatment in Male Rats with Chronic Inflammation

2013 ◽  
Vol 20 (2) ◽  
pp. 119-125 ◽  
Author(s):  
Gabriela Laste ◽  
Joanna Ripoll Rozisky ◽  
Isabel Cristina de Macedo ◽  
Vinicius Souza dos Santos ◽  
Izabel Cristina Custódio de Souza ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Chronic Inflammation ◽  
Neurotrophic Factor ◽  
Brain Derived Neurotrophic Factor ◽  
Male Rats ◽  
Dexamethasone Treatment

scholarly journals CORRELATION BETWEEN THE LYMPHOCYTE-MONOCYTE RATIO AND CYTOKINES IN CHRONIC INFLAMMATION IN RATS TREATED WITH ALLOGENEIC MESENCHYMAL STEM CELLS

2020 ◽  
Vol 7 (3) ◽  
pp. 109-117
Author(s):  
Nataliia Petryk ◽  
Oleksandr Shevchenko
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Chronic Inflammation ◽  
Pathological Condition ◽  
Male Rats ◽  
Tissue Destruction ◽  
Total Blood ◽  
Factors Affecting ◽  
Age Related ◽  
First Time

The chronic inflammatory process is a pathological condition characterized by an ongoing active inflammatory response and tissue destruction. Many studies show that chronic inflammation can play a severe role in various age-related diseases, including diabetes, cardiovascular, and autoimmune diseases. One of the important but poorly studied factors affecting the regulation of chronic inflammation is the regulatory activity of MSCs. In this regard, the study of mesenchymal stem cells preventing chronic inflammation in the experiment is an important area of modern pathology. On the one hand, increased cytokines, such as α-TNF, IL 6, and CRP, are reliable tools in diagnostic different inflammatory processes, especially chronic inflammation. On the other hand, nowadays, we need a more straightforward and not so expensive criterion for this purpose, for instance, a common total blood count and LMR. For the first time, we investigated how trustworthy can be LMR and how possible to use it in chronic inflammation in rats to achieve prognostic goals.  This study investigated the correlation between α-TNF, IL-6, and CRP with LMR in rats' plasma in groups with chronic carrageenan inflammation and chronic inflammation with local injection of MSCs into the affected area. The study involved 132 adult male rats (180-220g), which were divided into groups. The inflammation model was chronic aseptic myositis caused by an intramuscular injection of 10mg λ-carrageenan (Sigma-Aldrich GmbH). Our experimental groups of rats were treated with MSCs (the injection into the inflamed site) in the amount of 1-2 million cells once. Blood sampling was performed from 6 hours to 28 days. We calculated our results using Statistica (data analysis software) version 13. For comparison, we used one-way ANOVA, Turkey's post hoc test, where p <0.05 was considered statistically significant.  In our experiment, the correlation between levels of α-TNF, IL-6, and CRP with lymphocyte-monocyte ratio in rats was described for the first time, demonstrating the suppression of chronic inflammation through MSCs.

The Morphology of the Rat Kidney Following Folic Acid Administration

1970 ◽  
Vol 28 ◽  
pp. 200-201
Author(s):  
Aline Byrnes ◽  
Elsa E. Ramos ◽  
Minoru Suzuki ◽  
E.D. Mayfield
Keyword(s):  
Folic Acid ◽  
De Novo ◽  
Specific Activity ◽  
Rat Kidney ◽  
Present Report ◽  
Intracellular Localization ◽  
Male Rats ◽  
Renal Hypertrophy ◽  
Electron Microscopic ◽  
Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

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