An in vitro model quantifying the effect of calcification on the tissue–stent interaction in a stenosed aortic root

2020 ◽

Vol 4 (2) ◽

Author(s):

Jonathan J Morrison ◽

Hossam Abdou ◽

Michael Richmond ◽

Marta J Madurska ◽

Noha Elansary

Keyword(s):

Aortic Root ◽

In Vitro Model ◽

Swine Model ◽

Peristaltic Pump ◽

Extracorporeal Circuit ◽

Extracorporeal Circuits ◽

Vitro Model ◽

Aortic Cannula ◽

Set Up

Background: To demonstrate the utility and applicability of in vitro extracorporeal circuits in endovascular resuscitation research. Methods: The method for building an inexpensive in vitro extracorporeal circuit for endovascular resuscitation research is described. In this study, aortic cannulas and pump combinations were evaluated in the in vitro extracorporeal circuit. Then one aortic cannula and pump set up was evaluated in a post-mortem swine model. Flow data was collected and compared among groups. Results: The peristaltic pump generated the highest flow as compared to the other pump combinations at any given catheter size. The peristaltic pump combined with the 10 Fr cannula produced the highest flow overall at 2304 mL/min. This same combination produced a peak flow of 886 ml/min at the aortic root in the swine model. Conclusions: The flow generated in the swine model was less than half of that generated in the in vitro model. However, all flow was channeled through one outflow tract in the in vitro model whereas the swine aorta has several branches of outflow. As such, a 50% reduction in flow or greater is anticipated at the level of the aortic root. An in vitro extracorporeal circuit for endovascular research can be built for less than $10,000, with most of the materials being reusable, and can be used to generate representative data that may be anticipated in a swine model.

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Three-dimensional flow structures past a bio-prosthetic valve in an in-vitro model of the aortic root

PLoS ONE ◽

10.1371/journal.pone.0194384 ◽

2018 ◽

Vol 13 (3) ◽

pp. e0194384 ◽

Cited By ~ 6

Author(s):

David Hasler ◽

Dominik Obrist

Keyword(s):

Aortic Root ◽

Prosthetic Valve ◽

In Vitro Model ◽

Three Dimensional ◽

Flow Structures ◽

Three Dimensional Flow ◽

Dimensional Flow ◽

Vitro Model

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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