Production and characterization of extracellular beta-glucosidase from Pichia anomala MDD24 for enhancing wine aroma

Oak alternatives (OAs) such as chips, granulates or staves, are becoming increasingly used in the wine industry. Although they are mostly considered for their contribution to wine aroma, they are also a source of phenolic compounds to be released in the wine, in particular ellagitannins contributing to wine mouthfeel and antioxidant characteristics. In the present study, we explore the potential for a rapid analytical method based on linear sweep voltammetry (LSV) combined with disposable sensors to provide a rapid measure of the oxidizable compounds present in different OAs, as well as their characterization. Strong correlations were found between the tannin content of different OAs and the total current measures during LSV analysis, allowing a rapid quantification of the oxidizable compounds present, mostly ellagitannin. Application of derivatization to raw voltammograms allowed extraction of a number of features that can be used for classification purposes, in particular with respect to OAs types (chips or staves) and degree of toasting.

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Characterization of the sugar alcohol-producing yeast Pichia anomala

Journal of Industrial Microbiology & Biotechnology ◽

10.1007/s10295-013-1364-5 ◽

2013 ◽

Vol 41 (1) ◽

pp. 41-48 ◽

Cited By ~ 14

Author(s):

Guoqiang Zhang ◽

Yuping Lin ◽

Peng He ◽

Lin Li ◽

Qinhong Wang ◽

...

Keyword(s):

Pichia Anomala ◽

Sugar Alcohol

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Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

Chemical Papers ◽

10.1515/chempap-2015-0111 ◽

2015 ◽

Vol 69 (8) ◽

Cited By ~ 10

Author(s):

Alesandra Oriente ◽

Robson Tramontina ◽

Diandra de Andrades ◽

Caroline Henn ◽

Jose L. C. Silva ◽

...

Keyword(s):

Aspergillus Niger ◽

Lignocellulosic Biomass ◽

Culture Conditions ◽

Industrial Wastes ◽

Fermentation Inhibitors ◽

State Culture ◽

Solid State Culture ◽

Atlantic Rainforest Biome ◽

Beta Glucosidase

AbstractProperties of beta-glucosidase produced by Aspergillus niger URM 6642 recently isolated from the Atlantic rainforest biome and its potential tolerance to saccharification of lignocellulosic biomass products and fermentation inhibitors was evaluated. The fungus was cultivated under solid state culture conditions at 37°C with different agro-industrial wastes. High levels of beta-glucosidase (3778.9 U g

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Structural and Catalytic Characterization of TsBGL, a β-Glucosidase From Thermofilum sp. ex4484_79

Frontiers in Microbiology ◽

10.3389/fmicb.2021.723678 ◽

2021 ◽

Vol 12 ◽

Author(s):

Anke Chen ◽

Dan Wang ◽

Rui Ji ◽

Jixi Li ◽

Shaohua Gu ◽

...

Keyword(s):

Catalytic Domain ◽

Specific Activity ◽

Maximum Activity ◽

Homologous Proteins ◽

Glycosidic Bonds ◽

Catalytic Sites ◽

Potential Applications ◽

Beta Glucosidase ◽

Hydrolysis Of

Beta-glucosidase is an enzyme that catalyzes the hydrolysis of the glycosidic bonds of cellobiose, resulting in the production of glucose, which is an important step for the effective utilization of cellulose. In the present study, a thermostable β-glucosidase was isolated and purified from the Thermoprotei Thermofilum sp. ex4484_79 and subjected to enzymatic and structural characterization. The purified β-glucosidase (TsBGL) exhibited maximum activity at 90°C and pH 5.0 and displayed maximum specific activity of 139.2μmol/min/mgzne against p-nitrophenyl β-D-glucopyranoside (pNPGlc) and 24.3μmol/min/mgzen against cellobiose. Furthermore, TsBGL exhibited a relatively high thermostability, retaining 84 and 47% of its activity after incubation at 85°C for 1.5h and 90°C for 1.5h, respectively. The crystal structure of TsBGL was resolved at a resolution of 2.14Å, which revealed a classical (α/β)8-barrel catalytic domain. A structural comparison of TsBGL with other homologous proteins revealed that its catalytic sites included Glu210 and Glu414. We provide the molecular structure of TsBGL and the possibility of improving its characteristics for potential applications in industries.

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