Lignocellulosic support selection for colonization and ligninolytic enzyme production of white-rot fungus Anthracophyllum discolor

2010 ◽  
Vol 150 ◽  
pp. 279-280 ◽  
Author(s):  
Sebastian Elgueta ◽  
Maria Cristina Diez
Keyword(s):  
Enzyme Production ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Rot Fungus ◽  
Selection For

scholarly journals White-rot fungus: an updated review

2021 ◽  
pp. 202-217
Author(s):  
Jaspreet Kaur ◽  
Amar Pal Singh ◽  
Ajeet Pal Singh ◽  
Rajinderpal Kaur
Keyword(s):  
Stainless Steel ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Mold ◽  
White Rot Fungus ◽  
Impact Factors ◽  
Production Processes ◽  
Polyamide Fiber

The White Fungus, which causes white rot on tree trunks, belongs to the basidiomycetes. Research into the microbiology of White-rot fungi has focused on engineering processes related to factors such as cell growth and enzyme production processes, and to smaller, i.e., molecular biology. Many studies have been conducted to select issues with high or specific biodegradation performance in a variety of ways. Production inhibitors have been used to improve enzyme production. Investigators are investigating different carriers (Stainless Steel net, polyamide fiber net, fiberglass net and polyurethane foam) to impair P.chrysosporium ligninolytic enzyme production. In this review, Pathophysiology, Microbiology, impact factors, treatments and alternative uses show white mold formation in biotransformation. The white fungus is being investigated to produce biotechnology for the reduction of a broad spectrum, a natural pollutant based on lignin-deficient enzymes. This in particular covers the destruction of many wastes and environmental pollution, including wastewater, pesticides, toxic natural pollutants, chlorinated hydrocarbons, etc. It will be updated.

scholarly journals Light Regulation of Two New Manganese Peroxidase-Encoding Genes in Trametes polyzona KU-RNW027

2020 ◽  
Vol 8 (6) ◽  
pp. 852
Author(s):  
Piyangkun Lueangjaroenkit ◽  
Emi Kunitake ◽  
Makiko Sakka ◽  
Tetsuya Kimura ◽  
Churapa Teerapatsakul ◽  
...  
Keyword(s):  
Blue Light ◽  
Enzyme Production ◽  
Molecular Mechanisms ◽  
Light Regulation ◽  
Ligninolytic Enzyme ◽  
Regulatory Elements ◽  
White Rot ◽  
White Rot Fungus ◽  
Gene Expressions ◽  
Encoding Genes

To better understand the light regulation of ligninolytic systems in Trametes polyzona KU-RNW027, ligninolytic enzymes-encoding genes were identified and analyzed to determine their transcriptional regulatory elements. Elements of light regulation were investigated in submerged culture. Three ligninolytic enzyme-encoding genes, mnp1, mnp2, and lac1, were found. Cloning of the genes encoding MnP1 and MnP2 revealed distinct deduced amino acid sequences with 90% and 86% similarity to MnPs in Lenzites gibbosa, respectively. These were classified as new members of short-type hybrid MnPs in subfamily A.2 class II fungal secretion heme peroxidase. A light responsive element (LRE), composed of a 5′-CCRCCC-3′ motif in both mnp promoters, is reported. Light enhanced MnP activity 1.5 times but not laccase activity. The mnp gene expressions under light condition increased 6.5- and 3.8-fold, respectively. Regulation of laccase gene expression by light was inconsistent with the absence of LREs in their promoter. Blue light did not affect gene expressions but impacted their stability. Reductions of MnP and laccase production under blue light were observed. The details of the molecular mechanisms underlying enzyme production in this white-rot fungus provide useful knowledge for wood degradation relative to illumination condition. These novel observations demonstrate the potential of enhancing ligninolytic enzyme production by this fungus for applications with an eco-friendly approach to bioremediation.

scholarly journals Characterization of ligninolytic enzyme production in white-rot wild fungal strains suitable for kraft pulp bleaching

3 Biotech ◽  
2017 ◽  
Vol 7 (5) ◽  
Author(s):  
Rosa María Damián-Robles ◽  
Agustín Jaime Castro-Montoya ◽  
Jaime Saucedo-Luna ◽  
Ma. Soledad Vázquez-Garcidueñas ◽  
Marina Arredondo-Santoyo ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Kraft Pulp ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Pulp Bleaching ◽  
Fungal Strains ◽  
Kraft Pulp Bleaching

Ligninolytic enzyme ability and potential biotechnology applications of the white-rot fungus Grammothele subargentea LPSC no. 436 strain

2008 ◽  
Vol 43 (4) ◽  
pp. 368-375 ◽  
Author(s):  
Mario C.N. Saparrat ◽  
Paulina Mocchiutti ◽  
Constanza S. Liggieri ◽  
Mónica B. Aulicino ◽  
Néstor O. Caffini ◽  
...  
Keyword(s):  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Decolorisation of Azo Dye Congo Red (CR) by Termitomyces sp. Biomass

2021 ◽  
pp. 118-127
Author(s):  
Kavitha Mary Jackson ◽  
Velu Gomathi
Keyword(s):  
Congo Red ◽  
Fungal Biomass ◽  
Azo Dye ◽  
Tamil Nadu ◽  
Block Design ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Cell

Aims: A study was conducted to evaluate decoloration of azo dye, Congo Red (CR) using fungal hyphal mat of beneficial bacidiomycete Termitomyces sp. TMS7 (MW694830) as bio sorbent material. Study design:  Completely randomized block design (CRD). Place and duration of study: Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India, between September 2019 and January 2020. Methodology: Isolation of white rot fungus from basidiocarb was done and screened based on their ligninolytic enzyme activity and Isolate TMS 7 was selected as best isolate and identified through ITS 1 and ITS 4 primers. Efficiency of fungal biomass to decolorize Congo red was assessed and per cent decoloration and kinetics were calculated. Results: Twelve fungal isolates were obtained and Isolate TMS 7 was selected as best isolate based on enzymatic activity. TMS 7 was identified as Termitomyces sp. using ITS 1 and ITS 4 primer. Ligninolytic enzymes i.e. cellulase (9.97 µ mol of glucose released/min/mg protein), and xylanase (9.55 µ mol of xylose released/min/mg protein) were quantified from the crude fungal extract of TMS 7, which was higher than standard (Termitomyces albuminosus -MTCC 1366). Decolorisation efficiency of termitomyces fungal biomass (1 g/100 ml) against different concentration of congo red dye (50-250 mg/L) was assessed. About 100 % (99.9) degradation was recorded in the minimum dye concentration of 50 mg/L within 3 days and 8 % decoloration was achieved at the highest dye concentration (250 mg/L) within 5 days. Conclusion: Possible mechanism of degradation is the presence of lignolytic enzyme especially cellulase, xylanase in the culture filtrate and bio sorption of degraded product by the fungal cell wall components viz., chitin, glucan other complex polymers.

Effect of Moisture Content and Inoculum Size on Cell Wall Composition and Ethanol Yield from Switchgrass after Solid-State Pleurotus ostreatus Treatment

2018 ◽  
Vol 61 (6) ◽  
pp. 1997-2006 ◽  
Author(s):  
Mengxing Li ◽  
Stephen M. Marek ◽  
Jiaqi Peng ◽  
Zhongdong Liu ◽  
Mark R. Wilkins
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Perennial Grass ◽  
Ligninolytic Enzyme ◽  
Inoculum Size ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Pretreatment

Abstract. The white-rot fungus is capable of selectively degrading lignin over polymeric sugars. Solid-state cultivation and subsequent simultaneous saccharification and fermentation for ethanol production were performed. Effects of moisture content (MC) and fungus inoculum on biomass degradation, ligninolytic enzyme, and ethanol production were evaluated. First, fungal pretreatment was performed with varied MC and inoculum levels and sampled every 20 days. The highest xylose yield observed was 15.6% for samples with 75% MC and 5 mL inoculum at fungal pretreatment of 40 days. The highest lignin degradation of 52% and highest ethanol yield of 31% (based on the glucan present in the raw switchgrass) were achieved for 80-day fungal-treated samples with 75% MC and 5 mL inoculum. Keywords: KLywords. Bioenergy, Fungal pretreatment, Oyster mushroom, Perennial grass.

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