Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida

2012 ◽  
Vol 161 (2) ◽  
pp. 71-79 ◽  
Author(s):  
Sonja Christina Troeschel ◽  
Stephan Thies ◽  
Olga Link ◽  
Catherine Isabell Real ◽  
Katja Knops ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Broad Host Range ◽  
Shuttle Vectors ◽  
Expression In Escherichia Coli

Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors

Gene ◽  
1983 ◽  
Vol 26 (2-3) ◽  
pp. 273-282 ◽  
Author(s):  
Miroslawa M. Bagdasarian ◽  
Egon Amann ◽  
Rudolf Lurz ◽  
Beate Rückert ◽  
Michael Bagdasarian
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Expression Vectors ◽  
Broad Host Range ◽  
Tac Promoter

Broad-host-range application of the srfA promoter from Bacillus subtilis in Escherichia coli

2020 ◽  
Vol 168 ◽  
pp. 105798 ◽  
Author(s):  
Chengran Guan ◽  
Yan Ma ◽  
Xuan Chen ◽  
Ruifeng Zhao ◽  
Xinyuan Huang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Host Range ◽  
Broad Host Range

scholarly journals Expanding the toolbox of broad host-range transcriptional terminators for Proteobacteria through metagenomics

2018 ◽  
Author(s):  
Vanesa Amarelle ◽  
Ananda Sanches-Medeiros ◽  
Rafael Silva-Rocha ◽  
María-Eugenia Guazzaroni
Keyword(s):  
Escherichia Coli ◽  
Synthetic Biology ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Broad Host Range ◽  
Functional Metagenomics ◽  
Alternative Hosts ◽  
E Coli ◽  
Broad Host Range Plasmid

AbstractAs the field of synthetic biology moves towards the utilization of novel bacterial chassis, there is a growing need for biological parts with enhanced performance in a wide number of hosts. Is not unusual that biological parts (such as promoters and terminators), initially characterized in the model bacteria Escherichia coli, do not perform well when implemented in alternative hosts, such as Pseudomonas, therefore limiting the construction of synthetic circuits in industrially relevant bacteria. In order to address this limitation, we present here the mining of transcriptional terminators through functional metagenomics to identify novel parts with broad host-range activity. Using a GFP-based terminator trap strategy and a broad host-range plasmid, we identified 20 clones with potential terminator activity in Pseudomonas putida. Further characterization allowed the identification of 4 unique sequences between 58 bp and 181 bp long that efficiently terminates transcription in P. putida, E. coli, Burkholderia phymatum and two Pseudomonas strains isolated from Antarctica. Therefore, this work presents a new set of biological parts useful for the engineering of synthetic circuits in Proteobacteria.

Analysis of transcription from the trfA promoter of broad host range plasmid RK2 in Escherichia coli, Pseudomonas putida, and Pseudomonas aeruginosa

Plasmid ◽  
1987 ◽  
Vol 17 (3) ◽  
pp. 222-232 ◽  
Author(s):  
Michael Pinkney ◽  
Bimal D.M. Theophilus ◽  
Simon R. Warne ◽  
William C.A. Tacon ◽  
Christopher M. Thomas
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Pseudomonas Putida ◽  
Host Range ◽  
Broad Host Range ◽  
Broad Host Range Plasmid ◽  
Plasmid Rk2

scholarly journals Bacillus subtilis phenylalanyl-tRNA synthetase genes: cloning and expression in Escherichia coli and B. subtilis.

1989 ◽  
Vol 171 (2) ◽  
pp. 1228-1232 ◽  
Author(s):  
A A Brakhage ◽  
H Putzer ◽  
K Shazand ◽  
R J Röschenthaler ◽  
M Grunberg-Manago
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Trna Synthetase ◽  
Cloning And Expression ◽  
Expression In Escherichia Coli
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