Objective:
A validated liquid chromatography-tandem mass spectrometry method (LCMS/
MS) was established to simultaneously determine the concentration of triflusal and its main
metabolite 2-hydroxy-4-trifluoromethyl benzoic acid(HTB) in human urine.
Methods:
The separation was performed on a Dikma C18 column using isocratic elution with
acetonitrile-4 mmol/L ammonium acetate aqueous solution containing 0.3 % formic acid water (78: 28,
V/V). The method involved extraction with methanol using protein precipitation. The precursor-toproduct
ion transitions with multiple reaction monitoring was m/z 247.1→161.1, 204.8→106.7and
136.9→93.0 for triflusal, HTB and salicylic acid(IS), respectively. The method showed good linear
relationships over the ranges of 0.08 to 48 μg/mL and0.5 to 50 μg/mL.
Results:
It was the first time that a urinary excretion study of triflusal capsule as oral. The cumulative
urinary recovery showed 8.5% and 2.7% for triflusal and HTB, respectively.
Conclusion:
This method was successfully used for evaluating the pharmacokinetic properties of triflusal
and HTB in urine in Chinese healthy subjects.
Pharmacokinetics, tissue distribution and excretion study of a furostanol glycoside-based standardized fenugreek seed extract in rats