Potentiometric DNA sensing platform using redox-active DNA probe pair for sandwich-type dual hybridization at indicator electrode surface

2014 ◽  
Vol 720-721 ◽  
pp. 71-75 ◽  
Author(s):  
Koji Nakano ◽  
Takayuki Kimura ◽  
Yusuke Kitamura ◽  
Toshihiro Ihara ◽  
Ryoichi Ishimatsu ◽  
...  
The Analyst ◽  
2014 ◽  
Vol 139 (9) ◽  
pp. 2193-2198 ◽  
Author(s):  
Yun Zhang ◽  
Fang Liu ◽  
Jinfang Nie ◽  
Fuyang Jiang ◽  
Caibin Zhou ◽  
...  

This paper describes for the first time an electrochemical biosensor, which employs a DNA probe modified with a redox tag close to electrode surface, for picomolar detection of a sequence-specific DNA-binding protein.


2015 ◽  
Vol 87 (3) ◽  
pp. 1861-1866 ◽  
Author(s):  
Scott P. White ◽  
Kevin D. Dorfman ◽  
C. Daniel Frisbie

2020 ◽  
Vol 8 (7) ◽  
pp. 1389-1395 ◽  
Author(s):  
Fu-Ting Wang ◽  
Yi-Han Wang ◽  
Jing Xu ◽  
Ke-Jing Huang

A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification.


2019 ◽  
Vol 312 ◽  
pp. 432-440 ◽  
Author(s):  
Yolina Hubenova ◽  
Eleonora Hubenova ◽  
Blagoy Burdin ◽  
Daria Vladikova ◽  
Mario Mitov

2017 ◽  
Vol 164 (6) ◽  
pp. H345-H351 ◽  
Author(s):  
Dan Cheng ◽  
Linlin Yang ◽  
Xi Li ◽  
Jian Zhou ◽  
Qi Chen ◽  
...  

2015 ◽  
Vol 18 (3) ◽  
pp. 135-144
Author(s):  
Tien Huu Cao ◽  
Linh Van Ha ◽  
Hieu Van Le

The optimization of DNA probe immobilisation on gold electrode surface is very important to develop DNA biosensors. In this study, we conducted an experiment to determine the optimal concentration of probe attached on the electrodes and probe immobilization agent (mercaptohexanol) for maximum hybridization efficacy. We have used a method to control the surface density of DNA probe by annealing probe modified by thiol and mercaptohexanol. With linear relationship between molar ratio and surface density of probe, by controlling probe concentration in sensor fabricating process, we can determine the molecular density of DNA probes on electrode surface. The results show that probe concentration 500 nM and 1.5 mM mercaptohaxenol are optimal for hybidization with DNA target.


The Analyst ◽  
2019 ◽  
Vol 144 (9) ◽  
pp. 3023-3029 ◽  
Author(s):  
Qianqian Pei ◽  
Xiaolei Song ◽  
Su Liu ◽  
Jingfeng Wang ◽  
Xueqi Leng ◽  
...  

A facile signal-on electrochemical DNA biosensor for ultrasensitive detection of pathogenic bacteria based on Exo III-assisted autonomous multiple-cycle amplification.


Sign in / Sign up

Export Citation Format

Share Document