Effect of culture conditions on biomass yield of acclimatized microalgae in ozone pre-treated tannery effluent: A simultaneous exploration of bioremediation and lipid accumulation potential

2020 ◽  
Vol 273 ◽  
pp. 111129
Author(s):  
D. Saranya ◽  
S. Shanthakumar
Keyword(s):  
Lipid Accumulation ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Tannery Effluent ◽  
Accumulation Potential

Effect of nickel(II) on the biomass yield of the activated sludge

1996 ◽  
Vol 34 (5-6) ◽  
pp. 163-171 ◽  
Author(s):  
Celal F. Gökçay ◽  
Ulku Yetis
Keyword(s):  
Activated Sludge ◽  
Decay Constant ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Synthetic Wastewater ◽  
Base Line ◽  
Microbial Culture ◽  
Feed Composition ◽  
Steady State Condition ◽  
Applied Microbiology

Biomass yield of microorganisms is important in applied microbiology since it is the ultimate factor determining the amount of product produced regardless of whether product is growth-linked or not. In the case of environmental microbiology the opposite is true and minimizing the biomass produced, or the sludge in the relevant jargon, often is the prime goal. In this paper, a unique means of manipulating the microbial biomass yield of a heterogeneous culture to fulfil either of the two goals is presented. 5.0 mgl−1 Ni(II) in the feed composition to a completely mixed, once- through, activated sludge was found to induce the observed biomass yield of the microbial culture developed from sewage. As compared with the base-line study without Ni(II), where the reactor received synthetic wastewater only, true biomass yield was found to have increased along with the increased decay constant with the net effect of lowering observed biomass yield drastically at lower dilution rates and increasing it over that observed in the base-line study at higher dilution rates. At 10.0 mgl−1 influent Ni(II) concentration the culture conditions almost reverted back to the base- line study and at 25 mgl−1 Ni(II) concentration a truly steady-state condition could not be attained.

scholarly journals CD36 inhibition prevents lipid accumulation and contractile dysfunction in rat cardiomyocytes

2012 ◽  
Vol 448 (1) ◽  
pp. 43-53 ◽  
Author(s):  
Yeliz Angin ◽  
Laura K. M. Steinbusch ◽  
Peter J. Simons ◽  
Sabrina Greulich ◽  
Nicole T. H. Hoebers ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fatty Acid ◽  
Glucose Uptake ◽  
Lipid Accumulation ◽  
Insulin Signalling ◽  
Culture Conditions ◽  
Contractile Dysfunction ◽  
Rat Cardiomyocytes ◽  
Akt Phosphorylation ◽  
Palmitate Uptake

An increased cardiac fatty acid supply and increased sarcolemmal presence of the long-chain fatty acid transporter CD36 are associated with and contribute to impaired cardiac insulin sensitivity and function. In the present study we aimed at preventing the development of insulin resistance and contractile dysfunction in cardiomyocytes by blocking CD36-mediated palmitate uptake. Insulin resistance and contractile dysfunction were induced in primary cardiomyocytes by 48 h incubation in media containing either 100 nM insulin (high insulin; HI) or 200 μM palmitate (high palmitate; HP). Under both culture conditions, insulin-stimulated glucose uptake and Akt phosphorylation were abrogated or markedly reduced. Furthermore, cardiomyocytes cultured in each medium displayed elevated sarcolemmal CD36 content, increased basal palmitate uptake, lipid accumulation and decreased sarcomere shortening. Immunochemical CD36 inhibition enhanced basal glucose uptake and prevented elevated basal palmitate uptake, triacylglycerol accumulation and contractile dysfunction in cardiomyocytes cultured in either medium. Additionally, CD36 inhibition prevented loss of insulin signalling in cells cultured in HP, but not in HI medium. In conclusion, CD36 inhibition prevents lipid accumulation and lipid-induced contractile dysfunction in cardiomyocytes, but probably independently of effects on insulin signalling. Nonetheless, pharmacological CD36 inhibition may be considered as a treatment strategy to counteract impaired functioning of the lipid-loaded heart.

scholarly journals Overexpression of the Transcription Factor AtLEC1 Significantly Improved the Lipid Content of Chlorella ellipsoidea

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiao Liu ◽  
Dan Zhang ◽  
Jianhui Zhang ◽  
Yuhong Chen ◽  
Xiuli Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Content ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Expression Profiles ◽  
Fatty Acid Content ◽  
Culture Conditions ◽  
Chlorella Ellipsoidea ◽  
Promising Source ◽  
Transgenic Strains

Microalgae are considered to be a highly promising source for the production of biodiesel. However, the regulatory mechanism governing lipid biosynthesis has not been fully elucidated to date, and the improvement of lipid accumulation in microalgae is essential for the effective production of biodiesel. In this study,LEAFY COTYLEDON1 (LEC1)fromArabidopsis thaliana, a transcription factor (TF) that affects lipid content, was transferred intoChlorella ellipsoidea. Compared with wild-type (WT) strains, the total fatty acid content and total lipid content ofAtLEC1transgenic strains were significantly increased by 24.20–32.65 and 22.14–29.91%, respectively, under mixotrophic culture conditions and increased by 24.4–28.87 and 21.69–30.45%, respectively, under autotrophic conditions, while the protein content of the transgenic strains was significantly decreased by 18.23–21.44 and 12.28–18.66%, respectively, under mixotrophic and autotrophic conditions. Fortunately, the lipid and protein content variation did not affect the growth rate and biomass of transgenic strains under the two culture conditions. According to the transcriptomic data, the expression of 924 genes was significantly changed in the transgenic strain (LEC1-1). Of the 924 genes, 360 were upregulated, and 564 were downregulated. Based on qRT-PCR results, the expression profiles of key genes in the lipid synthesis pathway, such asACCase,GPDH,PDAT1, andDGAT1, were significantly changed. By comparing the differentially expressed genes (DEGs) regulated byAtLEC1inC. ellipsoideaandArabidopsis, we observed that approximately 59% (95/160) of the genes related to lipid metabolism were upregulated inAtLEC1transgenicChlorella. Our research provides a means of increasing lipid content by introducing exogenous TF and presents a possible mechanism ofAtLEC1regulation of lipid accumulation inC. ellipsoidea.

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Tian-Hua Zhong ◽  
Jia-Wei Zhang ◽  
Wei Xu ◽  
Liying Huang ◽  
Zhu-Hua Luo
Keyword(s):  
Cell Growth ◽  
Lipid Accumulation ◽  
Oil Production ◽  
Culture Conditions ◽  
Medium Composition ◽  
Open Ocean ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Fermentation Conditions ◽  
Oleaginous Fungus

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

scholarly journals Optimization of Chlorella Culture Conditions with Response Surface Methodology to Increase Biomass

2021 ◽  
Vol 20 (5) ◽  
Author(s):  
R. Kanimozhi ◽  
D. Arvind Prasath ◽  
R. Dhandapani ◽  
Santhosh Sigamani
Keyword(s):  
Response Surface Methodology ◽  
Light Intensity ◽  
Response Surface ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Accession Number ◽  
Microalgal Biomass ◽  
Chlorella Sp

Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response surface methodology (RSM) and validate further the optimized parameters. The optimum conditions for the cultivation of Chlorella sp. KPU016 under optimized nutrient conditions were pH 8.2, the light intensity of 3100 lx, glycerol 1.44 g.L-1 (under pre-set conditions of 12 h lighting, the temperature at 27±1°C. With these RSM-driven optimum conditions, the yield of microalgal biomass achieved was 282.50 mg.L-1. For larger-scale microalgal harvesting, the validated optimal conditions can be inferred as the best for enhanced microalgal production. The isolate was partially sequenced and submitted to the NCBI database and the GenBank accession number is MZ348364.

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