Inhibitory effect of individual and mixtures of nitrophenols on anaerobic toxicity assay of anaerobic systems: Metabolism and evaluation modeling

AbstractCandida albicans is fungus capable of changing from yeast to filamentous form when it’s transformed from a normal commensal to an opportunistic pathogen. The development of alternatives that interfere with this transition could be an effective way to reduce candidiasis. In this regard, evaluate the inhibitory effect of two Borojoa patinoi silver nanoparticles (AgNPs) produced by green synthesis at 5 °C and 25 °C on the process of filamentation of Candida albicans. The percentage of inhibition of filamentous forms of C. albicans ATCC10231 and C. albicans SC5314 with AgNPs was determined. Results showed that temperature of synthesis affected both the shape and size of silver nanoparticles synthesized using Borojoa patinoi extracts. The inhibition percentage of filamentous forms of Candida albicans ATCC10231 when treated with silver nanoparticles synthesized at 5 °C was 85.9% and at 25 °C it was 40%. C. albicans SC5314 when treated with AgNP synthesized at 5 °C was 97.2% and at 25 °C it was 64%. Cell toxicity assay showed that at 100ng/ml, AgNPs synthesized at 25 °C were safe in MES-OV CRL-3272 cell line. Our results showed that the silver nanoparticles obtained from Borojoa patinoi are inhibitors of the filamentous process of C. albicans.

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Design, Synthesis, and Docking Studies of Arylhydrazone Compounds and Evaluation of Their Platelet Aggregation Inhibitory Effect and Cytotoxicity

10.21203/rs.3.rs-1204698/v1 ◽

2022 ◽

Author(s):

Maryam H. Klidsar ◽

Marjan Esfahanizadeh ◽

Pantea Haghverdi ◽

Salimeh Amidi ◽

Farzad Kobarfard

Keyword(s):

Isonicotinic Acid ◽

Catalytic Amount ◽

Adenosine Diphosphate ◽

Inhibitory Effect ◽

Docking Studies ◽

Antiplatelet Activity ◽

Toxicity Assay ◽

Phenyl Hydrazine ◽

Design Synthesis ◽

Induced Aggregation

Abstract In view of proven antiplatelet activity of hydrazone group containing compounds, two series of hydrazone derivatives were synthesized by coupling appropriate aldehydes with phenyl hydrazine and Isonicotinic acid in the presence of distilled water and a catalytic amount of glacial acetic acid. All synthesized compounds were screened for their antiplatelet activity against induced aggregation by adenosine diphosphate (ADP) and arachidonic acid (AA). The results indicate that compounds in arylhydrazone group had shown satisfactory activity. Among them, 1-(3-methoxybenzylidene)-2-phenylhydrazine (1c), 2-methoxy-4-(2-phenylhydrazono) methyl phenol (1g), and 2-((2-phenylhydrazono) methyl)-1H-pyrrole (1h) were found to be the most potent antiplatelet compounds with IC50 less than 39 μM. Furthermore, the cell toxicity assay, (MTT test) indicates their noncytotoxic in various cell lines. None of the synthesized N-isonicotinohydrazide derivatives in this study excreted sufficient antiplatelet activity.

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Bioluminescence Toxicity Assay of Polyethylenimine-Based Sorbents

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-3-73-81 ◽

2020 ◽

Vol 36 (3) ◽

pp. 73-81

Author(s):

L.E. Aleskerova ◽

S.G. Vasilieva ◽

A.S. Morozov ◽

A.D. Ismailov ◽

E.S. Lobakova

Keyword(s):

Inhibitory Effect ◽

Diglycidyl Ether ◽

Photobacterium Phosphoreum ◽

Lower Percentage ◽

Russian Science ◽

Toxicity Assay ◽

Polymer Sorbents ◽

Luminescence Inhibition ◽

Toxicity Analysis ◽

Ethanol Extracts

The toxicity of polyethylenimine-based sorbents and their extracts was evaluated and their effect on the bioluminescence of Photobacterium phosphoreum photobacteria was studied. These test bacteria are commonly used as objects to determine the toxicity of various materials. The analyzed materials were synthesized by cross-linking PEI with diethylene glycol diglycidyl ether (DGDE) at mass contents of the latter of 1.9-120.0% with subsequent freezing. It was found that the degree of luminescence inhibition in the P. phosphoreum cells depended on the PEI/DGDE ratio in the sorbent. The sorbents with high DGDE content (60-120%) did not affect the cell luminescence activity, while those with a lower percentage of the cross-linker (0.9-30%) exerted a pronounced inhibitory effect on luminescence of photobacteria according to the data obtained via the standard biotesting method. It was also established that the inhibitory effect of sorbents with a lower DGDE percentage (<30%) in a phosphate buffer was significantly lower than in salt solutions. Water and ethanol extracts of sorbents with the DGDE mass percentage of more than 15% did not significantly inhibit the luminescence of P. phosphoreum during 1 h of incubation. Immobilization of P. phosphoreum cells on the surface and internal parts of the studied sorbents was observed by the method of scanning electron microscopy. bioluminescence, biotest, toxicity analysis, photobacteria, polymer sorbents, polyethylenimine This study was funded by the Russian Science Foundation (Grant no. 16-14-00112).

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