In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice

2010 ◽  
Vol 131 (2) ◽  
pp. 478-484 ◽  
Author(s):  
Nurmuhammat Amat ◽  
Halmurat Upur ◽  
Biljana Blažeković
Keyword(s):  
Aqueous Extract ◽  
Hepatoprotective Activity ◽  
Artemisia Absinthium ◽  
Liver Injuries

scholarly journals Isolation and in vivo hepatoprotective activity of Melothria heterophylla (Lour.) Cogn. against chemically induced liver injuries in rats

2011 ◽  
Vol 4 (8) ◽  
pp. 619-623 ◽  
Author(s):  
Arijit Mondal ◽  
Tapan Kumar Maity ◽  
Dilipkumar Pal ◽  
Santanu Sannigrahi ◽  
Jagadish Singh
Keyword(s):  
Hepatoprotective Activity ◽  
Chemically Induced ◽  
Liver Injuries

scholarly journals AMELIORATION OF ACETAMINOPHEN-INDUCED HEPATOTOXICITY USING SOLANUM TORVUM SW. FRUIT EXTRACT IN WISTAR RATS

2019 ◽  
pp. 238-242
Author(s):  
UMERA BEGAM AK ◽  
SENTHILKUMAR R ◽  
SIRAJUDEEN J
Keyword(s):  
Aqueous Extract ◽  
Tca Cycle ◽  
Hepatoprotective Activity ◽  
Marker Enzymes ◽  
Fruit Extract ◽  
Solanum Torvum ◽  
Medicinal Uses ◽  
Membrane Bound ◽  
Histopathological Studies

Objective: Ayurveda and Chinese pharmacopeia have highlighted the traditional medicinal uses of Solanum torvum Sw. The fruits are ethnomedical used in the treatment of liver and spleen enlargement, cough, and also used as a hematopoietic, antimicrobial, and analgesic agent. In the present study, the amelioration of acetaminophen (APAP)-induced hepatotoxicity of the aqueous extract of S. torvum Sw. fruits is evaluated. Methods: The hepatoprotective activity of the fruit extract against APAP insult was evaluated by assessing it is in vivo antioxidants status, membrane-bound adenosine triphosphatases (ATPases), and tricarboxylic acid (TCA) cycle marker enzymes and also through histopathological studies of the liver. Results: Administration of the aqueous fruit extract of the plant caused a significant increase in the in vivo antioxidant status as evident from the reduction in lipid peroxidation caused by APAP and improvement in the mitochondrial membrane stability which is proved from the activity of membrane-bound ATPases and TCA cycle marker enzymes. Histological studies also supported the fact that the plant extract proved to revive the architecture of the toxin damaged liver tissues in par with silymarin. The chemical pathological changes were consistent with histopathological observations suggesting marked hepatoprotective effect of the aqueous extract of S. torvum. Conclusion: The results showed that the extract of S. torvum Sw. fruits has hepatoprotective potential which may be due to the antioxidant activity of its phytoconstituents, especially flavonoids, alkaloids, phenolics, etc.

scholarly journals Evaluation of the Effectiveness ofPiper cubebaExtract in the Amelioration of CCl4-Induced Liver Injuries and Oxidative Damage in the Rodent Model

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Mansour AlSaid ◽  
Ramzi Mothana ◽  
Mohammad Raish ◽  
Mohammed Al-Sohaibani ◽  
Mohammed Al-Yahya ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Mrna Expression ◽  
Antioxidant Activities ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Rodent Model ◽  
Dependent Manner ◽  
Drug Induced ◽  
Liver Injuries

Background. Liver diseases still represent a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases including liver. Thus, the present study was to evaluate the effectiveness ofPiper cubebafruits in the amelioration of CCl4-induced liver injuries and oxidative damage in the rodent model.Methods. Hepatoprotective activity was assessed using various biochemical parameters like SGOT, SGPT,γ-GGT, ALP, total bilirubin, LDH, and total protein. Meanwhile,in vivoantioxidant activities as LPO, NP-SH, and CAT were measured in rat liver as well as mRNA expression of cytokines such as TNFα, IL-6, and IL-10 and stress related genes iNOS and HO-1 were determined by RT-PCR. The extent of liver damage was also analyzed through histopathological observations.Results. Treatment with PCEE significantly and dose dependently prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, PCEE significantly reduced the lipid peroxidation in the liver tissue and restored activities of defense antioxidant enzymes NP-SH and CAT towards normal levels. The administration of PCEE significantly downregulated the CCl4-induced proinflammatory cytokines TNFαand IL-6 mRNA expression in dose dependent manner, while it upregulated the IL-10 and induced hepatoprotective effect by downregulating mRNA expression of iNOS and HO-1 gene.

scholarly journals In Vitro and In Vivo Experimental Hepatotoxic Models in Liver Research: Applications to the Assessment of Potential Hepatoprotective Drugs

2016 ◽  
pp. S417-S425 ◽  
Author(s):  
H. FARGHALI ◽  
M. KGALALELO KEMELO ◽  
L. WOJNAROVÁ ◽  
N. KUTINOVÁ CANOVÁ
Keyword(s):  
Hepatoprotective Activity ◽  
Sirtuin 1 ◽  
In Vivo Model ◽  
In Vivo Models ◽  
Product Removal ◽  
Liver Injuries

This mini-review highlights our and others’ experience about in vitro and in vivo models that are being used to follow up events of liver injuries under various hepatotoxic agents and potential hepatoprotective drugs. Due to limitations of the outcomes in each model, we focus primarily on two models. First, a developed perfusion method for isolated immobilized hepatocytes that improves the process of oxygenation and helps in end-product removal is of considerable value in improving cell maintenance. This cellular model is presented as a short-term research-scale laboratory bioreactor with various physiological, biochemical, molecular, toxicological and pharmacological applications. Second, the in vivo model of D-galactosamine and lipopolysaccharide (D-GalN/LPS) combination-induced liver damage is described with some details. Recently, we have revealed that resveratrol and other natural polyphenols attenuate D-GalN/LPS-induced hepatitis. Moreover, we reported that D-GalN/LPS down-regulates sirtuin 1 in rat liver. Therefore, we discuss here the role of sirtuin 1 modulation in hepatoprotection. A successful development of pharmacotherapy for liver diseases depends on the suitability of in vitro and in vivo hepatic injury systems. Several models are available to screen the hepatotoxic or hepatoprotective activity of any substance. It is important to combine different methods for confirmation of the findings.

In vitro and in vivo Trypanocidal Effect of the Aqueous extract of the Leaves of Ochna Schweinfurthiana

2020 ◽  
Vol 4 (1) ◽  
pp. 47-51
Author(s):  
Eteme Enama S ◽  
Messi A N ◽  
Mahob R J ◽  
Siama A ◽  
Njan Nloga A M
Keyword(s):  
Aqueous Extract

Tinospora cordifolia Aqueous Extract Alleviates Cyclophosphamide- Induced Immune Suppression, Toxicity and Systemic Candidiasis in Immunosuppressed Mice: In vivo Study in Comparison to Antifungal Drug Fluconazole

2019 ◽  
Vol 20 (12) ◽  
pp. 1055-1063 ◽  
Author(s):  
Faris Alrumaihi ◽  
Khaled S. Allemailem ◽  
Ahmad Almatroudi ◽  
Mohammed A. Alsahli ◽  
Arif Khan ◽  
...  
Keyword(s):  
Survival Rate ◽  
Aqueous Extract ◽  
Survival Data ◽  
Tinospora Cordifolia ◽  
Systemic Candidiasis ◽  
Organ Index ◽  
Tnf Α ◽  
Fungal Load ◽  
Enhanced Secretion

Objective: The present study was aimed to evaluate the effect of the aqueous extract of Tinospora cordifolia (AETC) against cyclophosphamide-induced immunosuppression and systemic Candida albicans infection in a murine model. Methods: The protective effect of AETC against cyclophosphamide-induced leukopenia was evaluated by quantitative and qualitative analysis of the leukocytes. The immune-stimulating potential of AETC on macrophages was assessed by determining the levels of secreted cytokines. To determine the direct antifungal activity, AETC or fluconazole was administered to C. albicans infected mice. The efficacy of treatment was assessed by determining the survival rate, kidney fungal burden, the organ index and liver inflammation parameters. Results: Cyclophosphamide administration resulted in substantial depletion of leukocytes, whereas AETC treatment induced the recovery of leukocytes in cyclophosphamide-injected mice. Moreover, AETC treatment of macrophages resulted in enhanced secretion of IFN-γ, TNF-α and IL-1β. C. albicans infected mice treated with AETC at the doses of 50 and 100 mg/kg exhibited 40% and 60% survival rate, whereas the mice treated with fluconazole at a dose of 50 mg/kg showed 20% survival rate. Like survival data, the fungal load was found to be the lowest in the kidney tissues of mice treated with AETC at a dose of 100 mg/kg. Interestingly, mice infected with C. albicans demonstrated improvement in the organ indices and liver functioning after AETC treatment. Conclusion: These results suggest that AETC may potentially be used to rejuvenate the weakened immune system and eliminate systemic candidiasis in mice.

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

Sign in / Sign up

Export Citation Format

Share Document