scholarly journals AMELIORATION OF ACETAMINOPHEN-INDUCED HEPATOTOXICITY USING SOLANUM TORVUM SW. FRUIT EXTRACT IN WISTAR RATS

2019 ◽  
pp. 238-242
Author(s):  
UMERA BEGAM AK ◽  
SENTHILKUMAR R ◽  
SIRAJUDEEN J
Keyword(s):  
Aqueous Extract ◽  
Tca Cycle ◽  
Hepatoprotective Activity ◽  
Marker Enzymes ◽  
Fruit Extract ◽  
Solanum Torvum ◽  
Medicinal Uses ◽  
Membrane Bound ◽  
Histopathological Studies

Objective: Ayurveda and Chinese pharmacopeia have highlighted the traditional medicinal uses of Solanum torvum Sw. The fruits are ethnomedical used in the treatment of liver and spleen enlargement, cough, and also used as a hematopoietic, antimicrobial, and analgesic agent. In the present study, the amelioration of acetaminophen (APAP)-induced hepatotoxicity of the aqueous extract of S. torvum Sw. fruits is evaluated. Methods: The hepatoprotective activity of the fruit extract against APAP insult was evaluated by assessing it is in vivo antioxidants status, membrane-bound adenosine triphosphatases (ATPases), and tricarboxylic acid (TCA) cycle marker enzymes and also through histopathological studies of the liver. Results: Administration of the aqueous fruit extract of the plant caused a significant increase in the in vivo antioxidant status as evident from the reduction in lipid peroxidation caused by APAP and improvement in the mitochondrial membrane stability which is proved from the activity of membrane-bound ATPases and TCA cycle marker enzymes. Histological studies also supported the fact that the plant extract proved to revive the architecture of the toxin damaged liver tissues in par with silymarin. The chemical pathological changes were consistent with histopathological observations suggesting marked hepatoprotective effect of the aqueous extract of S. torvum. Conclusion: The results showed that the extract of S. torvum Sw. fruits has hepatoprotective potential which may be due to the antioxidant activity of its phytoconstituents, especially flavonoids, alkaloids, phenolics, etc.

scholarly journals HEPATOPROTECTIVE EVALUATION OF EPALTES DIVARICATA (L.) CASS. WHOLE PLANT EXTRACTS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS

2016 ◽  
Vol 8 (12) ◽  
pp. 231 ◽  
Author(s):  
K. Amala ◽  
R. Ilavarasan ◽  
R. Arunadevi ◽  
S. Amerjothy
Keyword(s):  
Aqueous Extract ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Dependent Manner ◽  
Traditional Use ◽  
Whole Plant ◽  
Medicinal Use ◽  
Histopathological Studies ◽  
Dose Dependent

<p><strong>Objective: </strong>The plant of <strong><em>Epaltes</em></strong><strong> <em>divaricata </em>(L.) </strong>Cass.<strong> Traditionally used for jaundice. </strong>The present work aimed to investigate the hepatoprotective activity of alcohol and aqueous extract of the whole plant against paracetamol-induced hepatotoxicity in rats to substantiate its traditional use.</p><p><strong>Methods: </strong>The alcohol and aqueous (200 and 400 mg/kg) extract of <em>Epaltes divaricata</em> prepared by cold maceration were administered orally to the animals with hepatotoxicity induced by paracetamol (1000 mg/kg). Silymarine (40 mg/k) was given as reference standard. Hepatoprotective activity was assessed by estimating marker enzymes and by histopathological studies.</p><p><strong>Results: </strong>Both alcohol and aqueous (200 and 400 mg/kg) extract treatment significantly restored the paracetamol-induced elevations in levels of serum enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphate (ALP) and total bilirubin in a dose-dependent manner. Histopathological examination revealed that the treatment attenuated the paracetamol-induced damage to the liver. The hepatoprotective effect of both extracts was comparable to that of the standard hepatoprotective agent, silymarin.</p><p><strong>Conclusion: </strong>The alcohol and aqueous extract of <em>E. divaricata</em> exhibited hepatoprotective effect against paracetamol-induced liver damage in rats. This study also validated their traditional medicinal use in jaundice.</p>

scholarly journals Quantitative Analysis and Pharmacological Effects of Artemisia ludoviciana Aqueous Extract and Compounds

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Isabel Rivero-Cruz ◽  
Gerardo Anaya-Eugenio ◽  
Araceli Pérez-Vásquez ◽  
Ana Laura Martínez ◽  
Rachel Mata
Keyword(s):  
Aqueous Extract ◽  
Antinociceptive Effect ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Hot Plate Test ◽  
Medicinal Uses ◽  
Inflammatory Phase ◽  
Artemisia Ludoviciana ◽  
Dose Dependent

Artemisia ludoviciana Nutt. (Asteraceae) is widely used in Mexican folk medicine for treating inflammation, diabetes and painful complaints. The in vivo antinociceptive, antiinflammatory and antihyperalgesic activities of an aqueous extract (AE) of the plant were investigated using well-known animal models. AE reduced the licking time in the formalin test in healthy and NA-STZ mice; the activity was better during the inflammatory phase; accordingly, it displayed significant antiinflammatory when tested at the same doses using the carrageenan-induced oedema model. AE also produced a significant dose-dependent antinociceptive effect in the hot plate test at 100 and 316 mg/kg (p.o.). Phytochemical analysis of the non-polar fraction of AE resulted in the isolation of two major lactones [achillin (1) and dehydroleucodin (2)], which showed antiinflammatory effect, being 2 the most active at 17.7 mg/kg. A suitable analytical method was successfully developed and validated to quantify 1 and 2. Altogether, these results tend to support the medicinal uses of the plant.

scholarly journals Hepatoprotective activity of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against CCl4-induced liver injury in rats

2019 ◽  
Vol 8 (8) ◽  
pp. 1758
Author(s):  
Naveen Kumar Bathula ◽  
Bimalendu Choudhary
Keyword(s):  
Alkaline Phosphatase ◽  
Superoxide Dismutase ◽  
Aqueous Extract ◽  
Liver Damage ◽  
Hepatoprotective Activity ◽  
Serum Markers ◽  
Hepatoprotective Agent ◽  
Histopathological Studies ◽  
The Impact ◽  
Dose Dependent

Background: The botanical Latin name of the plant, Turnera aphrodisiaca, describes its ancient use as an aphrodisiac.Methods: The aim of the present study is to evaluate the protective effect of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against carbon tetrachloride (CCl4)-induced liver damage in male Wistar rats.Results: Administration with ethanolic and aqueous extract of Turnera aphrodisiaca leaves (200 and 400 mg/kg) for 7 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in a dose dependent matter. In addition, treatment of both the extracts resulted in markedly increased the levels of superoxide dismutase and catalase enzymes in rats. The histopathological studies in the liver of rats also supported that both extracts markedly reduced the toxicity of CCL4 and preserved the histoarchitecture of the liver tissue to near normal.Conclusion: Thus, the results suggest that ethanolic and aqueous extract of  Turnera aphrodisiaca leaves acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats.

Evaluation of Hepatoprotective Effects of WrightiaTinctoria Leaves in Thioacetamide Induced Hepatotoxicity

2020 ◽  
Vol 11 (3) ◽  
pp. 3289-3293
Author(s):  
Nitin V Patil ◽  
Prakash M Somade ◽  
Pramod A. Patil ◽  
Atul R Chopade ◽  
Avinash V.Bhosale ◽  
...  
Keyword(s):  
Folk Medicine ◽  
Experimental System ◽  
Hepatoprotective Activity ◽  
Flavonoid Content ◽  
Serum Samples ◽  
Medicinal Use ◽  
Histopathological Studies ◽  
Phenolic And Flavonoid ◽  
Good Number

The plants belong to the genus Wrightiaare widely distributed throughout the world. Wrightiatinctoria (WT) plant species are being used in folk medicine for the treatment of several diseases. The review of the literature suggests that a good number of preclinical have confirmed the medicinal use of various Wrightiatinctoria species that have been mentioned in traditional medicine. The extract of Wrightiatinctoria was given daily to the rats, at doses of two hundred and 400 mg/kg along with thioacetamide to assess the affectivity of extract, against thioacetamide-induced hepatotoxicity. Serum samples were collected for analysis of various hepatoprotective parameters like aspartate transaminase, alanine transaminase, antacid phosphatase and total bilirubin, using commercially available test kits, together with morphological and histopathological indices in the liver of healthy and thioacetamide treated rats. Animals were sacrificed from each group, and their livers were dissected out for histopathological studies. Results of the present study suggest that the methanolic extract ofWrightiatinctoria leaves possess significant hepatoprotective activity on thioacetamide-induced hepatotoxicity, which might be associated with its high phenolic and flavonoid content and antioxidant properties.In conclusion, the present study depicts the curative efficacy of Wrightiatinctoria in an in-vivo experimental system. This study also revealed that Wrightiatinctoria could be used for the development of a phytomedicine against oxidative stress and liver ailments. However, further pharmacological evidence at the molecular level is required to establish the mechanism of the action of the drug.

scholarly journals EFFICACY OF WATTAKAKA VOLUBILIS AGAINST ALUMINIUM SULPHATE INDUCED IN LIVER TOXICITY

2018 ◽  
Vol 10 (2) ◽  
pp. 136
Author(s):  
Usharani S. ◽  
Anuradha R.
Keyword(s):  
Dna Fragmentation ◽  
Liver Toxicity ◽  
Tca Cycle ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Aluminium Sulphate ◽  
Metabolizing Enzymes ◽  
Membrane Bound ◽  
Medicinal Therapy ◽  
Careful Assessment

Objective: The modulation of membrane-bound ATPases, carbohydrate metabolizing enzymes and mitochondrial TCA cycle enzymes in Wattakaka volubilis on aluminium sulphate induced liver toxicity.Methods: Medicinal therapy requires careful assessment of effective treatment offering an acceptable safety over human health. Experimental animals were divided into five groups (Untreated, negative and positive control, hepatic group and the hepatic group fed on Wattakaka volubilis), Metabolising enzymes level, estimation of DNA, RNA and quantification of DNA fragmentation and gene expression were investigated. These altered enzyme levels were ameliorated significantly by administration of Wattakaka volubilis at the concentration of 200 mg/kg in drug-treated animals.Results: Results showed that treatment with methanol extract of Wattakaka volubilis normal level of enzymes which are compared with silymarin. This was evident from the significant increase in p<0.05, p<0.01, p<0.001 enzyme levels. Aluminium sulphate induced rats showed decreased the activities of metabolising enzymes and increased DNA fragmentation in the liver. This clearly explained the reason for the hepatoprotective activity of Wattakaka volubilis leaf extract.Conclusion: The methanolic leaf extract of W. volubilis showed high protective activity against aluminium sulphate-induced hepatotoxicity.

scholarly journals Evaluation of Hepatoprotective Activity and Oxidative Stress Reduction of Rosmarinus officinalis L. Shoots Tincture in Rats with Experimentally Induced Hepatotoxicity

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1737 ◽  
Author(s):  
Irina Ielciu ◽  
Bogdan Sevastre ◽  
Neli-Kinga Olah ◽  
Andreea Turdean ◽  
Elisabeta Chișe ◽  
...  
Keyword(s):  
Hepatoprotective Activity ◽  
Rosmarinus Officinalis ◽  
Raw Material ◽  
Carnosic Acid ◽  
Medicinal Uses ◽  
Antioxidant Mechanism ◽  
Rosmarinus Officinalis L ◽  
Experimentally Induced

Rosmarinus officinalis L. is a widely known species for its medicinal uses, that is also used as raw material for the food and cosmetic industry. The aim of the present study was to offer a novel perspective on the medicinal product originating from this species and to test its hepatoprotective activity. The tested sample consisted in a tincture obtained from the fresh young shoots. Compounds that are evaluated for this activity are polyphenols and terpenoids, that are identified and quantified by HPLC–UV–MS and GC–MS. Antioxidant activity was assessed in vitro, using the DPPH, FRAP and SO assays. Hepatoprotective activity was tested in rats with experimentally-induced hepatotoxicity. In the chemical composition of the tincture, phenolic diterpenes (carnosic acid, carnosol, rosmanol, rosmadial) and rosmarinic acid were found to be the majority compounds, alongside with 1,8-cineole, camphene, linalool, borneol and terpineol among monoterpenes. In vitro, the tested tincture proved significant antioxidant capacity. Results of the in vivo experiment showed that hepatoprotective activity is based on an antioxidant mechanism. In this way, the present study offers a novel perspective on the medicinal uses of the species, proving significant amounts of polyphenols and terpenes in the composition of the fresh young shoots tincture, that has proved hepatoprotective activity through an antioxidant mechanism.

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