Antidiabetic effect of total flavonoids from Sanguis draxonis in type 2 diabetic rats

This study was initiated to determine the possible antidiabetic effects of total flavonoids of Litsea Coreana leve (TFLC), an alcohol extract from the dried leaves of Litsea Coreana leve, on type 2 diabetic rats. Male Sprague-Dawley rats ( n = 40, 160–180 g) were divided into two groups and fed with normal chow diet (Normal Control group) or high-fat diet (HFD) for a period of 4 weeks. After 4 weeks of dietary manipulation, the HFD-fed rats were injected with 30 mg/kg streptozocin (STZ) to induce diabetes 72 hours after STZ injection. These diabetic rats were randomly divided into 3 groups ( n = 10): Diabetic Control group, Diabetic + TFLC group and Diabetic + PIO group. Diabetic + TFLC group and Diabetic + PIO group were orally administered with 400 mg/kg TFLC or 10 mg/kg pioglitazone (all suspended in 0.5% CMC-Na) respectively for 6 weeks. All rats were examined for body weight, serum and hepatic biochemical indices, content of malondialdehyde (MDA), activities of superoxide dismutase (SOD) and pathological changes in liver and pancreas, as well as protein tyrosine phosphatase 1B (PTP1B) expression in liver. The diabetic rats became obese, insulin resistant, hyperglycemic and hyperlipidemic. Treatment with TFLC showed a significant increase in insulin sensitivity, serum HDL-C level and SOD activities, meanwhile marked decrease in body weight, serum FFA, TC, TG, LDL-C, CRP, MDA content. TFLC also attenuated pathologic alterations in liver and pancreatic islet. Furthermore, TFLC was found to decrease the expression of PTP1B in diabetic rat liver. These results suggested that TFLC could ameliorate hyperglycemia, hyperlipoidemia, inflammation and oxidation stress, as well as insulin resistance of type 2 diabetic rats.

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Antidiabetic Effect ofMonolluma quadrangulaIs MediatedviaModulation of Glucose Metabolizing Enzymes, Antioxidant Defenses, and Adiponectin in Type 2 Diabetic Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/6290143 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 3

Author(s):

May N. Bin-Jumah

Keyword(s):

Insulin Sensitivity ◽

Glucose Tolerance ◽

Liver Lipid ◽

Diabetic Rats ◽

Antioxidant Defenses ◽

Metabolic Disturbances ◽

Metabolizing Enzymes ◽

Antidiabetic Effect ◽

Type 2 Diabetic

Monolluma quadrangulais a succulent bush traditionally used to treat diabetes and peptic ulcer. The present study aimed to investigate the effect ofM. quadrangulahydroethanolic extract on glucose tolerance, insulin sensitivity, glucose metabolizing enzymes, lipid profile, and adiponectin expression in type 2 diabetic rats. In addition, the study evaluated the antioxidant and anti-inflammatory activities of theM. quadrangulaextract. Type 2 diabetes was induced by feeding rats a high-fat diet (HFD) for 8 weeks followed by 30 mg/kg streptozotocin (STZ). Diabetic rats received 300 or 600 mg/kgM. quadrangulaextract for 4 weeks. HFD/STZ diabetic rats showed impaired glucose tolerance, reduced insulin secretion, and insulin resistance. HFD and STZ induced a significant increase in serum cholesterol, triglycerides and proinflammatory cytokines, and liver lipid peroxidation. Treatment withM. quadrangulaextract ameliorated these metabolic disturbances and increased liver glycogen, hexokinase activity, and antioxidants.M. quadranguladeclined the activity of liver glucose-6-phosphatase and fructose-1,6-biphosphatase. In addition,M. quadrangulaextract increased serum adiponectin levels and hepatic adiponectin expression in HFD/STZ diabetic rats. In conclusion,M. quadrangulaexerts antidiabetic effect mediatedviaameliorating glucose tolerance, insulin sensitivity, glucose metabolizing enzymes, and antioxidant defenses. Increased adiponectin levels and expression seems to mediate, at least in part, the antidiabetic effect ofM. quadrangula.

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